Abstract
Using the fluorescent Ca2+ probe Fura-2AM, the possible involvement of phospholipase A2, the key enzyme in the arachidonic acid cascade, in the effect of drugs glutoxim and molixan on the intracellular Ca2+ concentration in macrophages was studied. It was shown for the first time that preincubation of macrophages with the classical phospholipase A2 inhibitor, 4-bromophenacyl bromide, as well as with glucocorticosteroids prednisolone and dexamethasone significantly inhibits Ca2+ responses induced by glutoxim or molixan in macrophages. These results indicate the involvement of phospholipase A2 and arachidonic acid cascade in glutoximand molixan-induced signaling in macrophages.
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Original Russian Text © Z.I. Krutetskaya, L.S. Milenina, A.A. Naumova, V.G. Antonov, A.D. Nozdrachev, 2015, published in Doklady Akademii Nauk, 2015, Vol. 465, No. 2, pp. 249–251.
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Krutetskaya, Z.I., Milenina, L.S., Naumova, A.A. et al. Phospholipase A2 inhibitors modulate the effects of glutoxim and molixan on the intracellular Ca2+ level in macrophages. Dokl Biochem Biophys 465, 374–376 (2015). https://doi.org/10.1134/S1607672915060083
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DOI: https://doi.org/10.1134/S1607672915060083