Doklady Biochemistry and Biophysics

, Volume 465, Issue 1, pp 374–376 | Cite as

Phospholipase A2 inhibitors modulate the effects of glutoxim and molixan on the intracellular Ca2+ level in macrophages

  • Z. I. Krutetskaya
  • L. S. Milenina
  • A. A. Naumova
  • V. G. Antonov
  • A. D. Nozdrachev
Biochemistry, Biophysics and Molecular Biology


Using the fluorescent Ca2+ probe Fura-2AM, the possible involvement of phospholipase A2, the key enzyme in the arachidonic acid cascade, in the effect of drugs glutoxim and molixan on the intracellular Ca2+ concentration in macrophages was studied. It was shown for the first time that preincubation of macrophages with the classical phospholipase A2 inhibitor, 4-bromophenacyl bromide, as well as with glucocorticosteroids prednisolone and dexamethasone significantly inhibits Ca2+ responses induced by glutoxim or molixan in macrophages. These results indicate the involvement of phospholipase A2 and arachidonic acid cascade in glutoximand molixan-induced signaling in macrophages.


Arachidonic Acid GSSG Thapsigargin DOKLADY Biochemistry Arachidonic Acid Cascade 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • Z. I. Krutetskaya
    • 1
  • L. S. Milenina
    • 1
  • A. A. Naumova
    • 1
  • V. G. Antonov
    • 1
  • A. D. Nozdrachev
    • 1
  1. 1.St. Petersburg State UniversitySt. PetersburgRussia

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