Doklady Biochemistry and Biophysics

, Volume 465, Issue 1, pp 374–376 | Cite as

Phospholipase A2 inhibitors modulate the effects of glutoxim and molixan on the intracellular Ca2+ level in macrophages

  • Z. I. Krutetskaya
  • L. S. Milenina
  • A. A. Naumova
  • V. G. Antonov
  • A. D. Nozdrachev
Biochemistry, Biophysics and Molecular Biology

Abstract

Using the fluorescent Ca2+ probe Fura-2AM, the possible involvement of phospholipase A2, the key enzyme in the arachidonic acid cascade, in the effect of drugs glutoxim and molixan on the intracellular Ca2+ concentration in macrophages was studied. It was shown for the first time that preincubation of macrophages with the classical phospholipase A2 inhibitor, 4-bromophenacyl bromide, as well as with glucocorticosteroids prednisolone and dexamethasone significantly inhibits Ca2+ responses induced by glutoxim or molixan in macrophages. These results indicate the involvement of phospholipase A2 and arachidonic acid cascade in glutoximand molixan-induced signaling in macrophages.

Keywords

Arachidonic Acid GSSG Thapsigargin DOKLADY Biochemistry Arachidonic Acid Cascade 

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • Z. I. Krutetskaya
    • 1
  • L. S. Milenina
    • 1
  • A. A. Naumova
    • 1
  • V. G. Antonov
    • 1
  • A. D. Nozdrachev
    • 1
  1. 1.St. Petersburg State UniversitySt. PetersburgRussia

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