Doklady Biochemistry and Biophysics

, Volume 463, Issue 1, pp 232–235 | Cite as

Novel 3D-microcarriers from recombinant spidroin for regenerative medicine

  • M. M. Moisenovich
  • N. V. Malyuchenko
  • A. Y. Arkhipova
  • M. S. Kotlyarova
  • L. I. Davydova
  • A. V. Goncharenko
  • O. I. Agapova
  • M. S. Drutskaya
  • V. G. Bogush
  • I. I. Agapov
  • V. G. Debabov
  • M. P. Kirpichnikov
Biochemistry, Biophysics and Molecular Biology

Abstract

Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel particles ranging from 50 to 300 µm in size with the predominance of particles of 50–150 µm. The surface of these microparticles had a complex topography and ensured efficient cultivation of primary and immortalized fibroblasts. Intradermal injections of microgel suspensions into the area of full-thickness skin wounds did not lead to the development of acute inflammation in mice; instead, they accelerated the recovery of skin tissue and stimulated neurogenesis and angiogenesis.

Keywords

Regenerative Medicine Mouse Embryonic Fibroblast DOKLADY Biochemistry Intradermal Injection Microgel Particle 

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Copyright information

© Pleiades Publishing, Ltd. 2015

Authors and Affiliations

  • M. M. Moisenovich
    • 1
  • N. V. Malyuchenko
    • 1
  • A. Y. Arkhipova
    • 1
  • M. S. Kotlyarova
    • 1
  • L. I. Davydova
    • 2
  • A. V. Goncharenko
    • 3
  • O. I. Agapova
    • 4
  • M. S. Drutskaya
    • 5
  • V. G. Bogush
    • 2
  • I. I. Agapov
    • 4
  • V. G. Debabov
    • 2
  • M. P. Kirpichnikov
    • 1
  1. 1.Moscow State UniversityMoscowRussia
  2. 2.State Institute for Genetics and Selection of Industrial MicroorganismsPervyi Dorozhnyi proezdMoscowRussia
  3. 3.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  4. 4.Shumakov Research Institute of Transplantation and Artificial OrgansRussian Ministry of HealthMoscowRussia
  5. 5.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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