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Doklady Biochemistry and Biophysics

, Volume 449, Issue 1, pp 112–115 | Cite as

Molecular analysis of transcription start sites of separate copies of the suffix short retroelement in genome of Drosophila

  • O. S. Zenina
  • D. M. Fedoseeva
  • O. V. Kretova
  • N. A. Tchurikov
Biochemistry, Biophysics and Molecular Biology

Abstract

In order to study TSS in the suffix element, we used total RNA isolated from ovaries of Drosophila melanogaster. Using a 5′-RACE System (Invitrogen) and 454 sequencing, we found the full-length suffix sense transcripts. However, most 5′-RACE reads (>70%) correspond to 5′-truncated transcripts lacking the first 33–39 nucleotides. The data may indicate that these RNAs are products of the RNAi-related silencing mech-anism producing small RNAs that are larger than piRNAs. The full-length suffix transcripts could arise either from the F element or from active separate copies of suffix.

Keywords

5′-RACE suffix element TSS Drosophila deep sequencing 

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • O. S. Zenina
    • 1
  • D. M. Fedoseeva
    • 1
  • O. V. Kretova
    • 1
  • N. A. Tchurikov
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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