Doklady Biochemistry and Biophysics

, Volume 449, Issue 1, pp 72–74 | Cite as

New alternative promoter in regulation of the oct-1 human gene transcription

  • T. N. Portseva
  • I. D. Krylova
  • S. G. Georgieva
  • A. G. Stepchenko
  • E. V. Pankratova
Biochemistry, Biophysics and Molecular Biology


For the first time, the presence of a new alternative promoter in the gene of the oct-1 transcription factor from which a previously unknown mRNA isoform Oct-1X, with 5′-terminus different from the previously described isoforms, was demonstrated. The nucleotide sequence of the Oct-1X cDNA was determined and the presence of a long open reading frame which starts with the first ATG codon of the second exon was demonstrated. A protein shortened on its N-terminus is assumed to be the product of a new mRNA isoform. Expression of the new isoform was studied in various human cell lines; the results indicate the ubiquitous nature of the Oct-1X expression. Therefore, in the work, the third alternative promoter of human oct-1 gene (earlier, a ubiquitous promoter U and an inducible tissue-specific promoter L were described) contributing to the fine regulation of mRNA isoform synthesis and formation of structural and functional diversity of Oct-1 protein isoforms in cells was revealed.


Human Cell Line DOKLADY Biochemistry Alternative Promoter Breast Cancer Cell SKBR3 Ubiquitous Promoter 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Herr, W., Sturm, R.A., Clerc, R.G., et al., Genes Dev., 1988, vol. 2, pp. 1513–1516.PubMedCrossRefGoogle Scholar
  2. 2.
    Fletcher, C., Heintz, N., and Roeder, R.G., Cell, vol. 51, pp. 773–781.Google Scholar
  3. 3.
    Delhase, M., Castrillo, J.-L., de la Hoya, M., et al., J. Biol. Chem., 1996, vol. 271, pp. 32349–32358.PubMedCrossRefGoogle Scholar
  4. 4.
    Sytina, E.V. and Pankratova, E.V., Mol. Biol. (Moscow), 2003, vol. 37, no. 5, pp. 755–767.CrossRefGoogle Scholar
  5. 5.
    Kang, J., Goodman, B., Zheng, Y., and Tantin, D., PLoS ONE, 2011, vol. 6, e23872.PubMedCrossRefGoogle Scholar
  6. 6.
    Lin, B.R. and Natarajan, V., Gene, 2012, vol. 15, pp. 200–207.CrossRefGoogle Scholar
  7. 7.
    Voleti, B., Hammond, D.J., Jr., Thirumalai, A., and Agrawal, A., Mol. Immunol., 2012, vol. 52, pp. 242–248.PubMedCrossRefGoogle Scholar
  8. 8.
    Suzuki, N., Peter, W., Ciesiolka, T., et al., Nucleic Acids Res., 1993, vol. 21, pp. 245–252.PubMedCrossRefGoogle Scholar
  9. 9.
    Stepchenko, A.G., Nucleic Acids Res., 1992, vol. 20, p. 1419.CrossRefGoogle Scholar
  10. 10.
    Pankratova, E.V., Sytina, E.V., Stepchenko, A.G., et al., Mol. Biol. (Moscow), 2005, vol. 39, no. 6, pp. 816–823.CrossRefGoogle Scholar
  11. 11.
    Pankratova, E.V., Deev, I.E., Zhenilo, S.V., and Polanovsky, O.L., Mol. Genet. Genomics, 2001, vol. 266, pp. 239–245.PubMedCrossRefGoogle Scholar
  12. 12.
    Luchina, N.N., Krivega, I.V., and Pankratova, E.V., Immunol. Lett., 2003, vol. 85, pp. 237–241.PubMedCrossRefGoogle Scholar
  13. 13.
    Stepchenko, A.G., Pankratova, E.V., Doronin, S.A., et al., Nucleic Acids Res., 2011, vol. 39, pp. 5401–5411.PubMedCrossRefGoogle Scholar
  14. 14.
    Pankratova, E.V., Mol. Biol. (Moscow), 2008, vol. 42, no. 3, pp. 1–12.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • T. N. Portseva
    • 1
  • I. D. Krylova
    • 1
    • 2
  • S. G. Georgieva
    • 1
  • A. G. Stepchenko
    • 1
  • E. V. Pankratova
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Moscow State Pedagogical UniversityMoscowRussia

Personalised recommendations