Doklady Biochemistry and Biophysics

, Volume 449, Issue 1, pp 72–74 | Cite as

New alternative promoter in regulation of the oct-1 human gene transcription

  • T. N. Portseva
  • I. D. Krylova
  • S. G. Georgieva
  • A. G. Stepchenko
  • E. V. Pankratova
Biochemistry, Biophysics and Molecular Biology

Abstract

For the first time, the presence of a new alternative promoter in the gene of the oct-1 transcription factor from which a previously unknown mRNA isoform Oct-1X, with 5′-terminus different from the previously described isoforms, was demonstrated. The nucleotide sequence of the Oct-1X cDNA was determined and the presence of a long open reading frame which starts with the first ATG codon of the second exon was demonstrated. A protein shortened on its N-terminus is assumed to be the product of a new mRNA isoform. Expression of the new isoform was studied in various human cell lines; the results indicate the ubiquitous nature of the Oct-1X expression. Therefore, in the work, the third alternative promoter of human oct-1 gene (earlier, a ubiquitous promoter U and an inducible tissue-specific promoter L were described) contributing to the fine regulation of mRNA isoform synthesis and formation of structural and functional diversity of Oct-1 protein isoforms in cells was revealed.

Keywords

Human Cell Line DOKLADY Biochemistry Alternative Promoter Breast Cancer Cell SKBR3 Ubiquitous Promoter 

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • T. N. Portseva
    • 1
  • I. D. Krylova
    • 1
    • 2
  • S. G. Georgieva
    • 1
  • A. G. Stepchenko
    • 1
  • E. V. Pankratova
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Moscow State Pedagogical UniversityMoscowRussia

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