Slippage of the Primer Strand in the Primer Extension Reaction with Modified 2'-Deoxyuridine Triphosphates

Abstract

An effect of primer strand slippage was observed in the primer extension reaction due to replacement of thymidine triphosphate for modified analogs of dUTP. The effect was detected when using Vent (exo–) DNA polymerase. It is notably suppressed by adding to reaction mixture the natural nucleotides dATP, dCTP, and dGTP.

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Funding

This work was financially supported by the Federal Target Program “Research and Development in Priority Directions for the Development of the Russian Science and Technology Complex for 2014–2020” (agreement No. 14.576.21.0096, unique project identifier RFMEFI57617X0096).

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Correspondence to A. V. Chudinov.

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This article does not contain any research involving humans and animals as research objects.

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The authors declare they have no conflict of interest.

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Abbreviations: dUTP, 2'-deoxyuridine triphosphate; dTTP, thymidine triphosphate; dATP, 2'-deoxyadenosine triphosphate; dCTP, 2'-deoxycytidine triphosphate; dGTP, 2'-deoxyguanosine triphosphate; SELEX (systematic evolution of ligands by exponential enrichment) is the systematic evolution of ligands by exponential enrichment.

Corresponding author: phone: +7 (499) 135-98-00; fax +7 (495) 135-14-05; e-mail: chud@eimb.ru.

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Vasiliskov, V.A., Shershov, V.E., Miftahov, R.A. et al. Slippage of the Primer Strand in the Primer Extension Reaction with Modified 2'-Deoxyuridine Triphosphates. Russ J Bioorg Chem 46, 312–314 (2020). https://doi.org/10.1134/S106816202003022X

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Keywords:

  • modified nucleotides
  • primer extension
  • slippage