Production of the Extracellular Part of the ErbB2 Receptor for the Study of Immunobiologicals

Abstract

The extracellular part of the ErbB2 receptor (ecdErbB2), an oncological marker and a target for therapeutic antibodies, has been expressed in eukaryotic CHO cells. The extracellular part of the ErbB2 receptor has been isolated from a culture medium by metal affinity chromatography and characterized by biochemical and immunochemical methods. It has been shown that the receptor is produced in monomeric, dimeric, and trimeric forms. The oligomeric forms have been separated by gel filtration, and their ability to bind to Herceptin, one of the main drugs in the treatment of breast cancer, has been characterized by ELISA. The receptor produced can be used for in vitro immunochemical experiments.

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Funding

The study was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement no. 075-15-2019-1385 of 19.06.2019, unique project ID RFMEFI60417X0189).

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Correspondence to V. S. Rybchenko.

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The paper does not contain any studies involving animal or human participants performed by any of the authors.

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The authors declare that there is no conflict of interest.

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Translated by S. Sidorova

Abbreviations: ScFv–HSA, a single-chain variable fragment–human serum albumin; ecdErbB2, the extracellular part of ErbB2; PBS(T), phosphate-buffered saline with Tween-20; TBS(T), Tris-buffered saline with Tween-20.

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Rybchenko, V.S., Balabashin, D.S., Panina, A.A. et al. Production of the Extracellular Part of the ErbB2 Receptor for the Study of Immunobiologicals. Russ J Bioorg Chem 46, 327–333 (2020). https://doi.org/10.1134/S106816202003019X

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Keywords:

  • ErbB2
  • СНО
  • trastuzumab
  • SK-OV-3