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Russian Journal of Bioorganic Chemistry

, Volume 44, Issue 2, pp 217–224 | Cite as

Determination of Specific Class E Immunoglobulins to Bet v 1 Birch Allergen by the Immuno-PCR Method

  • M. A. Simonova
  • V. D. Pivovarov
  • D. Yu. Ryazantsev
  • M. A. Kostromina
  • T. I. Muravieva
  • M. A. Mokronosova
  • S. V. Khlgatian
  • R. S. Esipov
  • S. K. Zavriev
Article
  • 16 Downloads

Abstract

The aim of the work is the development of a method of detection of specific class E immunoglobulins (IgE) to the main Bet v 1 birch allergen based on immuno-PCR (iPCR). The recombinant Bet v 1 allergen was obtained in E. coli cells. Its ability to bind to specific IgE was confirmed by enzyme-linked immunosorbent assay (ELISA) using previously characterized sera of individuals with an allergic reaction to birch pollen and control sera in individuals, in which the reaction to this allergen is absent. Based on the obtained recombinant protein, the method of iPCR analysis of specific IgE to Bet v 1 was developed. It was demonstrated that iPCR sensitivity is comparable to ELISA sensitivity, and the titration curves of specific sera in iPCR (unlike those in ELISA) demonstrate a linear dependence; this makes the developed method preferable for quantitative estimation of specific IgE in sera as compared with ELISA.

Keywords

allergy class E immunoglobulin recombinant birch allergen serodiagnosis immuno-PCR 

Abbreviations

ELISA

enzyme-linked immunosorbent assay

PCR

polymerase chain reaction

IgE

class E immunoglobulin

IU

international unit

IPTG

isopropyl-β-D-1-thiogalactopyranoside

PMSF

phenylmethylsulfonyl fluoride

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • M. A. Simonova
    • 1
  • V. D. Pivovarov
    • 1
  • D. Yu. Ryazantsev
    • 1
  • M. A. Kostromina
    • 1
  • T. I. Muravieva
    • 1
  • M. A. Mokronosova
    • 2
  • S. V. Khlgatian
    • 3
  • R. S. Esipov
    • 1
  • S. K. Zavriev
    • 1
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Ado Allergic CenterMoscowRussia
  3. 3.Mechnikov Research Institute of Vaccines and SeraMoscowRussia

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