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Study of the Carbohydrate Specificity of Antibodies Against Aspergillus fumigatus Using the Library of Synthetic Mycoantigens

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Abstract

The carbohydrate specificity of antibodies obtained by immunizing laboratory animals with immunogens prepared from the Aspergillus fumigatus cell wall was analyzed using the library of biotinylated synthetic oligosaccharides. It has been shown that the main part of antipolysaccharide antibodies recognizes galactomannan in the studied immunogens with preference to its fragments containing more than two β-(1→5)-linked galactofuranosyl units. The data obtained can form the basis for the development of enzyme immunoassay for the detection of this dangerous fungal pathogen.

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Abbreviations

MWCO:

molecular weight cut-off

EDAC:

1-ethyl-3-(3-dimethylaminopropyl)carbodiimide

PBS:

phosphate-buffered saline.

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Authors and Affiliations

  1. Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia

    V. B. Krylov, M. I. Petruk, D. A. Argunov, E. A. Khatuntseva, M. V. Toplishek, B. S. Komarova, A. A. Karelin, O. N. Yudina, V. M. Menshov, D. V. Yashunskii, Y. E. Tsvetkov & N. E. Nifantiev

  2. XEMA Company Limited, Moscow, 105264, Russia

    I. V. Grigoryev & Y. S. Lebedin

  3. Kazan Scientific Research Institute of Epidemiology and Microbiology, Kazan, 420015, Russia

    N. I. Glushko & E. V. Khaldeeva

Authors
  1. V. B. Krylov
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  2. M. I. Petruk
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  3. I. V. Grigoryev
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  4. Y. S. Lebedin
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  5. N. I. Glushko
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  6. E. V. Khaldeeva
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  7. D. A. Argunov
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  8. E. A. Khatuntseva
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  9. M. V. Toplishek
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  10. B. S. Komarova
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  11. A. A. Karelin
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  12. O. N. Yudina
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  13. V. M. Menshov
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  14. D. V. Yashunskii
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  15. Y. E. Tsvetkov
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  16. N. E. Nifantiev
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Corresponding author

Correspondence to N. E. Nifantiev.

Additional information

Original Russian Text © V.B. Krylov, M.I. Petruk, I.V. Grigoryev, Y.S. Lebedin, N.I. Glushko, E.V. Khaldeeva, D.A. Argunov, E.A. Khatuntseva, M.V. Toplishek, B.S. Komarova, A.A. Karelin, O.N. Yudina, V.M. Menshov, D.V. Yashunskii, Y.E. Tsvetkov, N.E. Nifantiev, 2018, published in Bioorganicheskaya Khimiya, 2018, Vol. 44, No. 1, pp. 78–86.

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Krylov, V.B., Petruk, M.I., Grigoryev, I.V. et al. Study of the Carbohydrate Specificity of Antibodies Against Aspergillus fumigatus Using the Library of Synthetic Mycoantigens. Russ J Bioorg Chem 44, 80–89 (2018). https://doi.org/10.1134/S1068162017060073

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  • DOI: https://doi.org/10.1134/S1068162017060073

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