Studying the Sensitivity of Graphene for Biosensor Applications

Abstract

We have studied the response of graphene-film-based chips on SiC substrates (the relative change in the chip resistance) to coming into contact with fluorescein (C20H12O5) in a wide range of its concentrations in a phosphate-buffered saline solution: from 1 × 10–3 to 1 × 104 ng/mL (seven orders of magnitude). Fluorescein detection seems to be a simple and cheap model experiment to study the sensory ability of graphene in the way of biochips manufacturing. It has been shown that chips with wide terraces on a surface with a step width of about 1000 nm and heights of up to 5 nm made it possible to construct the calibration dependences of chip response on fluorescein concentration.

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REFERENCES

  1. 1

    K. S. Novoselov, A. K. Geim, S. V. Morozov, D. Jiang, Y. Zhang, S. V. Dubonos, I. V. Grigorieva, and A. A. Firsov, Science (Washington, DC, U. S.) 306, 666 (2004).

    ADS  Article  Google Scholar 

  2. 2

    Y. Wu, Y.-M. Lin, A. A. Bol, K. A. Jenkins, F. Xia, D. B. Farmer, Y. Zhu, and P. Avouris, Nature (London, U.K.) 472, 74 (2011).

    ADS  Article  Google Scholar 

  3. 3

    F. Schedin, A. K. Geim, S. V. Morozov, E. W. Hill, P. Blake, M. I. Katsnelson, and K. S. Novoselov, Nat. Mater. 6, 652 (2007).

    ADS  Article  Google Scholar 

  4. 4

    A. A. Lebedev, V. Yu. Davydov, S. N. Novikov, D. P. Litvin, Yu. N. Makarov, V. B. Klimovich, and M. P. Samoilovich, Tech. Phys. Lett. 42, 729 (2016). https://doi.org/10.1134/S1063785016070233

    ADS  Article  Google Scholar 

  5. 5

    I. M. Vlasova and A. M. Saletsky, Curr. Appl. Phys. 9, 1027 (2009).

    ADS  Article  Google Scholar 

  6. 6

    V. Yu. Davydov, D. Yu. Usachov, S. P. Lebedev, A. N. Smirnov, V. S. Levitskii, I. A. Eliseyev, P. A. Alekseev, M. S. Dunaevskiy, O. Yu. Vilkov, A. G. Rybkin, and A. A. Lebedev, Semiconductors 51, 1072 (2017). https://doi.org/10.1134/S1063782617080073

    ADS  Article  Google Scholar 

  7. 7

    V. Georgakilas, M. Otyepka, A. B. Bourlinos, V. Chandra, N. Kim, K. C. Kemp, P. Hobza, R. Zboril, and K. S. Kim, Chem. Rev. 112, 6156 (2012).

    Article  Google Scholar 

  8. 8

    Z. Tehrani, G. Burwell, M. A. Mohd Azmi, A. Castaing, R. Rickman, J. Almarashi, P. Dunstan, Beigi A. Miran, S. H. Doak, and O. J. Guy, 2D Mater. 1, 025004 (2014).

  9. 9

    A. Usikov, K. Borodkin, S. Novikov, A. Roenkov, A. Goryachkin, M. Puzyk, I. Barash, S. Lebedev, A. Zubov, Y. Makarov, and A. Lebedev, Proc. Eston. Acad. Sci. 68, 207 (2019). https://doi.org/10.3176/proc.2019.2.13

    Article  Google Scholar 

  10. 10

    S. Eissa, G. Contreras Jimenez, F. Mahvash, A. Guermoune, C. Tlili, T. Szkopek, M. Zourob, and M. Siaj, Nano Res. 8, 1698 (2015). https://doi.org/10.1007/s12274-014-0671-0

    Article  Google Scholar 

  11. 11

    Y. Pu, W. Wang, R. B. Dorshow, and R. R. Alfano, Proc. SPIE 8258, 825818 (2012). https://doi.org/10.1117/12.904692

    Article  Google Scholar 

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ACKNOWLEDGMENTS

The authors are grateful to P.A. Dement’ev for AFM measurements.

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Correspondence to A. S. Usikov.

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Translated by G. Dedkov

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Usikov, A.S., Lebedev, S.P., Roenkov, A.D. et al. Studying the Sensitivity of Graphene for Biosensor Applications. Tech. Phys. Lett. 46, 462–465 (2020). https://doi.org/10.1134/S1063785020050296

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Keywords:

  • graphene
  • silicon carbide
  • biosensor
  • fluorescein.