Studying the Sensitivity of Graphene for Biosensor Applications


We have studied the response of graphene-film-based chips on SiC substrates (the relative change in the chip resistance) to coming into contact with fluorescein (C20H12O5) in a wide range of its concentrations in a phosphate-buffered saline solution: from 1 × 10–3 to 1 × 104 ng/mL (seven orders of magnitude). Fluorescein detection seems to be a simple and cheap model experiment to study the sensory ability of graphene in the way of biochips manufacturing. It has been shown that chips with wide terraces on a surface with a step width of about 1000 nm and heights of up to 5 nm made it possible to construct the calibration dependences of chip response on fluorescein concentration.

This is a preview of subscription content, log in to check access.

Fig. 1.
Fig. 2.
Fig. 3.


  1. 1

    K. S. Novoselov, A. K. Geim, S. V. Morozov, D. Jiang, Y. Zhang, S. V. Dubonos, I. V. Grigorieva, and A. A. Firsov, Science (Washington, DC, U. S.) 306, 666 (2004).

    ADS  Article  Google Scholar 

  2. 2

    Y. Wu, Y.-M. Lin, A. A. Bol, K. A. Jenkins, F. Xia, D. B. Farmer, Y. Zhu, and P. Avouris, Nature (London, U.K.) 472, 74 (2011).

    ADS  Article  Google Scholar 

  3. 3

    F. Schedin, A. K. Geim, S. V. Morozov, E. W. Hill, P. Blake, M. I. Katsnelson, and K. S. Novoselov, Nat. Mater. 6, 652 (2007).

    ADS  Article  Google Scholar 

  4. 4

    A. A. Lebedev, V. Yu. Davydov, S. N. Novikov, D. P. Litvin, Yu. N. Makarov, V. B. Klimovich, and M. P. Samoilovich, Tech. Phys. Lett. 42, 729 (2016).

    ADS  Article  Google Scholar 

  5. 5

    I. M. Vlasova and A. M. Saletsky, Curr. Appl. Phys. 9, 1027 (2009).

    ADS  Article  Google Scholar 

  6. 6

    V. Yu. Davydov, D. Yu. Usachov, S. P. Lebedev, A. N. Smirnov, V. S. Levitskii, I. A. Eliseyev, P. A. Alekseev, M. S. Dunaevskiy, O. Yu. Vilkov, A. G. Rybkin, and A. A. Lebedev, Semiconductors 51, 1072 (2017).

    ADS  Article  Google Scholar 

  7. 7

    V. Georgakilas, M. Otyepka, A. B. Bourlinos, V. Chandra, N. Kim, K. C. Kemp, P. Hobza, R. Zboril, and K. S. Kim, Chem. Rev. 112, 6156 (2012).

    Article  Google Scholar 

  8. 8

    Z. Tehrani, G. Burwell, M. A. Mohd Azmi, A. Castaing, R. Rickman, J. Almarashi, P. Dunstan, Beigi A. Miran, S. H. Doak, and O. J. Guy, 2D Mater. 1, 025004 (2014).

  9. 9

    A. Usikov, K. Borodkin, S. Novikov, A. Roenkov, A. Goryachkin, M. Puzyk, I. Barash, S. Lebedev, A. Zubov, Y. Makarov, and A. Lebedev, Proc. Eston. Acad. Sci. 68, 207 (2019).

    Article  Google Scholar 

  10. 10

    S. Eissa, G. Contreras Jimenez, F. Mahvash, A. Guermoune, C. Tlili, T. Szkopek, M. Zourob, and M. Siaj, Nano Res. 8, 1698 (2015).

    Article  Google Scholar 

  11. 11

    Y. Pu, W. Wang, R. B. Dorshow, and R. R. Alfano, Proc. SPIE 8258, 825818 (2012).

    Article  Google Scholar 

Download references


The authors are grateful to P.A. Dement’ev for AFM measurements.

Author information



Corresponding author

Correspondence to A. S. Usikov.

Ethics declarations

The authors declare that they have no conflict of interest.

Additional information

Translated by G. Dedkov

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Usikov, A.S., Lebedev, S.P., Roenkov, A.D. et al. Studying the Sensitivity of Graphene for Biosensor Applications. Tech. Phys. Lett. 46, 462–465 (2020).

Download citation


  • graphene
  • silicon carbide
  • biosensor
  • fluorescein.