Russian Journal of Marine Biology

, Volume 44, Issue 2, pp 100–111 | Cite as

Fatty-Acid and Stable-Isotope Compositions in Shallow-Water Bivalve Mollusks and their Food

  • V. I. Kharlamenko
  • S. I. Kiyashko
Original Papers


We conducted a comparative analysis of the fatty acid (FA) composition and the ratios of stable isotopes of carbon (δ13C) and nitrogen (δ15N) in soft tissues of ten species of bivalve mollusks collected simultaneously on adjacent biotopes in shallow Vostok Bay (the Sea of Japan). Comparison of the FA composition of the lipids of digestive gland and all soft tissues showed that the percentages of C16 and C18 marker FAs were greater in the digestive gland and the levels of marker C20 and C22 FAs were, in most cases, higher in soft tissues. According to the results of cluster analysis and principal component analysis, four groups of samples were identified with a similarity of the FA composition of more than 80% within groups. The carbon stableisotope ratios varied within very wide limits in the studied species of bivalves; the range of δ13C variations was 8.1‰. The range of δ15N variations was much smaller, 2.5‰. Two pairs of species of mollusks (Saxidomus purpurataProtothaca euglypta and P. jedoensisDiplodonta semiasperoides) did not differ in the values of both δ15N and δ13C, the remaining species differed in at least one of these parameters. The greatest similarity of the FA composition and stable-isotope ratios was found in species that inhabit similar substrates, except Macoma irus and D. semiasperoides. Particularly marked differences in the FA composition and stable-isotope ratios were found between a filter-/surface deposit-feeder M. irus and filter-feeders Arca boucardi and Mytilus coruscus that live next to this species.


bivalves fatty acids stable isotopes sestonophages Vostok Bay Sea of Japan 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kuznetsov, A.P., On detritus feeding and seston feeding in the class Bivalvia, in Pitanie morskikh bespozvonochnykh v estestvennykh usloviyakh (Feeding of Marine Invertebrates Under Natural Conditions), Moscow: Inst. Okeanol., Akad. Nauk SSSR, 1986, pp. 6–10.Google Scholar
  2. 2.
    Morozova, T.V. and Orlova, T.Yu., Monitoring of phytoplankton in the area of a sea farm in Vostok Bay (Sea of Japan), Russ. J. Mar. Biol., 2005, vol. 31, no. 1, pp. 1–6.CrossRefGoogle Scholar
  3. 3.
    Silina, A.V. and Zhukova, N.V., Feeding and growth of Japanese scallop inhabiting different bottom sediment types, Biol. Bull. (Moscow), 2007, vol. 34, no. 1, pp. 55–60.CrossRefGoogle Scholar
  4. 4.
    Kharlamenko, V.I., Kiyashko, S.I., Rodkina, S.A., and Imbs, A.B., Determination of food sources of marine invertebrates from a subtidal sand community using analyses of fatty acids and stable isotopes, Russ. J. Mar. Biol., 2008, vol. 34, no. 2, pp. 101–109.CrossRefGoogle Scholar
  5. 5.
    Kharlamenko, V.I., Kiyashko, S.I., Rodkina, S.A., and Svetashev, V.I., The composition of fatty acids and stable isotopes in the detritophage Acila insignis (Gould, 1861) (Bivalvia: Nuculidae): searching for markers of a microbial food web, Russ. J. Mar. Biol., 2011, vol. 37, no. 3, pp. 201–208.CrossRefGoogle Scholar
  6. 6.
    Kharlamenko, V.I., Stepanov, V.G, Borisovets, E.E., et al., The fatty-acid composition and nutrition of deep-sea holothurians from the Sea of Okhotsk, Russ. J. Mar. Biol., 2015, vol. 41, no. 6, pp. 448–455.CrossRefGoogle Scholar
  7. 7.
    Tsikhon-Lukanina, E.A., Trofologiya vodnykh mollyuskov (Trophology of Aquatic Mollusks), Moscow: Nauka, 1987.Google Scholar
  8. 8.
    Ackman, R.G., Fatty acid metabolism of bivalves, in Biochemical and Physiological Approaches to Shellfish Nutrition, Second Int. Conf. Aquacult. Nutr., Baton Rouge: World Mariculture Soc. Spec. Publ., 1983, pp. 358–376.Google Scholar
  9. 9.
    Ackman, R.G., Tocher, C.S., and McLachlan, J., Marine phytoplankter fatty acids, J. Fish. Res. Board Can., 1968, vol. 25, pp. 1603–1620.CrossRefGoogle Scholar
  10. 10.
    Barnathan, G., Non-methylene-interrupted fatty acids from marine invertebrates: Occurrence, characterization and biological properties, Biochimie, 2009, vol. 91, no. 6, pp. 671–678.CrossRefPubMedGoogle Scholar
  11. 11.
    Bligh, E.G. and Dyer, W.J., A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 1959, vol. 37, no. 8, pp. 911–917.CrossRefPubMedGoogle Scholar
  12. 12.
    Budge, S.M. and Parrish, C.C., Lipid biogeochemistry of plankton, settling matter and sediments in Trinity Bay, Newfoundland. II. Fatty acids, Org. Geochem., 1998, vol. 29, no. 5–7, pp. 1547–1559.CrossRefGoogle Scholar
  13. 13.
    Carreau, J.P. and Dubacq, J.P., Adaptation of macroscale method to the micro-scale for fatty acid methyl transesterification of biological lipid extracts, J. Chromatogr. A, 1978, vol. 151, no. 3, pp. 384–390.CrossRefGoogle Scholar
  14. 14.
    Dalsgaard, J., St. John, M., Kattner, G., et al., Fatty acid trophic markers in the pelagic marine environment, Adv. Mar. Biol., 2003, vol. 46, pp. 225–340.CrossRefPubMedGoogle Scholar
  15. 15.
    Delaporte, M., Soudant, P., Moal, J., et al., Incorporation and modification of dietary fatty acids in gill polar lipids by two bivalve species Crassostrea gigas and Ruditapes philippinarum, Comp. Biochem. Physiol., Part A: Mol. Integr. Physiol., 2005, vol. 140, no. 4, pp. 460–470.CrossRefGoogle Scholar
  16. 16.
    Howell, K.L., Pond, D.W., Billett, D.S.M., et al., Feeding ecology of deep-sea seastars (Echinodermata: Asteroidea): A fatty-acid biomarker approach, Mar. Ecol.: Prog. Ser., 2003, vol. 255, pp. 193–206.CrossRefGoogle Scholar
  17. 17.
    Kelly, J.R. and Scheibling, R.E., Fatty acids as dietary tracers in benthic food webs, Mar. Ecol.: Prog. Ser., 2012, vol. 446, pp. 1–22.CrossRefGoogle Scholar
  18. 18.
    Kharlamenko, V.I., Kiyashko, S.I., Imbs, A.B., et al., Identification of food sources of invertebrates from the seagrass Zostera marina community using carbon and sulfur stable-isotope ratio and fatty acid analyses, Mar. Ecol.: Prog. Ser., 2001, vol. 220, pp. 103–117.CrossRefGoogle Scholar
  19. 19.
    Lang, I.K., Hodac, L., Friedl, T., et al., Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection, BMC Plant Biol., 2011, vol. 11, p.124.CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Makhutova, O.N., Protasov, A.A., Gladyshev, M.I., et al., Feeding spectra of bivalve mollusks Unio and Dreissena from Kanevskoe Reservoir, Ukraine: Are they food competitors or not?, Zool. Stud., 2013, vol. 52, p.56.CrossRefGoogle Scholar
  21. 21.
    Mansour, M.P., Holdsworth, D.G., Forbes, S.E., et al., High contents of 24:6(n-3) and 20:1(n-13) fatty acids in the brittle star Amphiura elandiformis from Tasmanian coastal sediments, Biochem. Syst. Ecol., 2005, vol. 33, no. 7, pp. 659–674.CrossRefGoogle Scholar
  22. 22.
    Napolitano, G.E. and Ackman, R.G., Fatty acid dynamics in sea scallops Placopecten magellanicus (Gmelin, 1791) from Georges bank, Nova Scotia, J. Shellfish Res., 1993, vol. 12, no. 2, pp. 267–277.Google Scholar
  23. 23.
    Napolitano, G.E., Pollero, R.J., Gayoso, A.M., et al., Fatty acids as trophic markers of phytoplankton blooms in the Bahia Blanca estuary (Buenos Aires, Argentina) and in Trinity Bay (Newfoundland, Canada), Biochem. Syst. Ecol., 1997, vol. 25, no. 8, pp. 739–755.CrossRefGoogle Scholar
  24. 24.
    Pasquaud, S., Lobry, J., and Elie, P., Facing the necessity of describing estuarine ecosystems: a review of food web ecology study techniques, Hydrobiologia, 2007, vol. 588, pp. 159–172.CrossRefGoogle Scholar
  25. 25.
    Perez, V., Olivier, F., Tremblay, R., et al., Trophic resources of the bivalve, Venus verrucosa, in the Chausey archipelago (Normandy, France) determined by stable isotopes and fatty acids, Aquat. Living Resour., 2013, vol. 26, no. 3, pp. 229–239.CrossRefGoogle Scholar
  26. 26.
    Perry, G.J., Volkman J.K., Johns R.B., et al., Fatty acids of bacterial origin in contemporary marine sediments, Geochim. Cosmochim. Acta, 1979, vol. 43, no. 11, pp. 1715–1725.CrossRefGoogle Scholar
  27. 27.
    Puccinelli, E., Noyon, M., and McQuaid, C.D., Trophic signatures of co-existing invasive and indigenous mussels: Selective feeding or different metabolic pathways? Hydrobiologia, 2017, vol. 784, no. 1, pp. 187–199.CrossRefGoogle Scholar
  28. 28.
    Saito, H. and Marty, Y., High levels of icosapentaenoic acid in the lipids of oyster Crassostrea gigas ranging over both Japan and France, J. Oleo Sci., 2010, vol. 59, no. 6, pp. 281–292.CrossRefPubMedGoogle Scholar
  29. 29.
    Sargent, J.R., Parkes, R.J., Mueller-Harvey, I., et al., Lipid biomarkers in marine ecology, in Microbes in the Sea, 1987, Chichester: Horwood, pp. 119–138.Google Scholar
  30. 30.
    Svetashev, V.I., Mild method for preparation of 4,4-dimethyloxazoline derivatives of polyunsaturated fatty acids for GC-MS, Lipids, 2011, vol. 46, no. 5, pp. 463–467.CrossRefPubMedGoogle Scholar
  31. 31.
    The LipidWeb, Mass spectrometry of fatty acid derivatives, 2017.
  32. 32.
    Wang, S.K., Jin, B.S., Qin, H.M., et al., Trophic dynamics of filter feeding bivalves in the Yangtze estuarine intertidal marsh: Stable isotope and fatty acid analyses, PLoS One, 2015, vol. 10, no. 8, p.21.Google Scholar
  33. 33.
    Zhao, L.Q., Yang, F., and Yan, X.W., Stable isotopes and fatty acids as dietary tracers of intertidal bivalves, Fish. Sci. (Tokyo, Jpn.), 2013, vol. 79, no. 5, pp. 749–756.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern BranchRussian Academy of SciencesVladivostokRussia
  2. 2.Far Eastern Federal UniversityVladivostokRussia

Personalised recommendations