Apoptosis of human lymphocytes, induced by exposure to UV light (240–390 nm) at a dose of 1510 J/m2, is accompanied by a significant increase in the intracellular level of reactive oxygen species for 1–2 hours after irradiation. The addition of caffeine (10–4 mol/L) and genistein (10–6 mol/L) to UV-irradiated lymphocytes induces a decrease in the number of cells at the early stage of apoptosis by 11 and 23%, respectively, as compared to the control sample. The antiapoptotic effects of caffeine and genistein in relation to photomodified lymphocytes are related to their ability to reduce the level of reactive oxygen species in intact cells and UV-irradiated cells, as well as to inactivate hydrogen peroxide. Verapamil (10–4 mol/L), in contrast to the effects of caffeine and genistein, does not reduce the number of lymphocytes that are at the early stage of apoptosis, as compared to that parameter for photomodified free cells, but it demonstrates the effects of reducing the levels of reactive oxygen species in immunocytes.
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Nakvasina, M.A., Tokmakova, E.V., Koltakov, I.A. et al. Antiapoptotic Effects of Caffeine, Genistein, and Verapamil in Relation to UV-Irradiated Lymphocyte Cells. Biol Bull Russ Acad Sci 47, 1547–1551 (2020). https://doi.org/10.1134/S1062359020110114
- UV light
- reactive oxygen species