Genetic Diversity of a Post-Cryogenic Fragaria vesca L. Clone after Long-Term Cultivation in vitro and Influenced by Abiotic Factors


The effects of bright light and high temperature stresses on the genetic diversity of a Fragaria vesca L. clone recovered from meristems frozen in liquid nitrogen after two years of cultivation in vitro were analyzed. After two-year cultivation in vitro of post-cryogenic plant material, the percentage of polymorphic DNA-markers, which was half (4.5%) of its initial value after cryopreservation, immediately returned to its initial value (9%). The Shannon index increased insignificantly. The stress effects of high temperature or bright sunlight stress effects on plantlets of the post-cryogenic clone recovered after two years liquid nitrogen did not cause the appearance of additional polymorphic fragments or significant changes in the Shannon Index.

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This work was supported by the Russian Foundation for Basic Research, project no. 14-04-31615.


The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Correspondence to A. I. Solov’eva.

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Translated by T. Borisova

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Solov’eva, A.I., Vysotskaya, O.N. Genetic Diversity of a Post-Cryogenic Fragaria vesca L. Clone after Long-Term Cultivation in vitro and Influenced by Abiotic Factors. Biol Bull Russ Acad Sci 47, 253–258 (2020).

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