The effects of bright light and high temperature stresses on the genetic diversity of a Fragaria vesca L. clone recovered from meristems frozen in liquid nitrogen after two years of cultivation in vitro were analyzed. After two-year cultivation in vitro of post-cryogenic plant material, the percentage of polymorphic DNA-markers, which was half (4.5%) of its initial value after cryopreservation, immediately returned to its initial value (9%). The Shannon index increased insignificantly. The stress effects of high temperature or bright sunlight stress effects on plantlets of the post-cryogenic clone recovered after two years liquid nitrogen did not cause the appearance of additional polymorphic fragments or significant changes in the Shannon Index.
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Akdemir, H., Suzerer, V., Tilkat, E., Yildirim, H., Onay, A., and Ciftci, Y.O., In vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm, J. Plant Biochem. Biotechnol., 2013, vol. 22, pp. 43–51.
Aronen, T.S., Krajnakova, J., Häggman, H.M., and Ryyäanen, L.A., Genetic fidelity of cryopreserved embryogenic cultures of open-pollinated Abies cephalonica,Plant Sci., 1999, vol. 142, pp. 163–172.
Bairu, M.W., Aremu, O.A., and van Staden, J., Somaclonal variation in plants: causes and detection methods, Plant Growth Regul., 2011, vol. 63, pp. 147–173.
Boyko, A., Greer, M., and Kovalchuk, I., Acute exposure to UVB has a more profound effect on plant genome stability than chronic exposure, Mutat. Res., 2006, vol. 606, pp. 100–109.
Castillo, F.V., Bassil, V., Wad, S., and Reed, M., Genetic stability of cryopreserved shoot tips of Rubus germplasm, In Vitro Cell Dev. Biol.—Plant, 2010, vol. 46, pp. 246–256.
Chalmers, K.J., Waugh, R., Sprent, J.I., Simons, A.J., and Powell, W., Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers, Heredity, 1992, vol. 69, pp. 465–472.
Cvjetko, P., Balen, B., Peharec, Š.P., Debogović, L., and Pavlica, M., Dynamics of heat-shock induced DNA damage and repair in senescent tobacco plants, Biol. Plant., 2014, vol. 58, pp. 71–79.
Dolgikh, Yu.I., Solov’eva, A.I., and Tereshonok, D.V., Variability of DNA markers in Arabidopsis thaliana cultured cells under standard growing conditions and under the influence of stressors, Biol. Bull. (Moscow), 2017, vol. 44, no. 2, pp. 120–127.
Gonzales-Benito, M.E., Kremer, C., Ibáñez, M.A., and Martin, C., Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation-dehydration, Plant Cell Tiss.Cult., 2016, vol. 127, pp. 359–368.
Govindaraj, M., Vetriventhan, M., and Srinivasan, M., Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives, Genet. Res. Int., 2015, art. ID 431487.
Haliem, E.A., Abdullah, H., and AL-Huqail, A.A., Oxidative damage and mutagenic potency of fast neutron and uv-b radiation in pollen mother cells and seed yield of Vicia faba L., BioMed Res. Int., 2013, art. ID 824656.
Kaity, A., Ashmore, S.E., and Drew, R.A., Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones, Plant Cell Rep., 2009, vol. 28, no. 9, pp. 1421–1430.
Kaity, A., Drew, R.A., and Ashmore, S.E., Genetic and epigenetic integrity assessment of acclimatized papaya plants regenerated directly from shoot-tips following short- and long-term cryopreservation, Plant Cell Tiss.Org., 2013, vol. 112, pp. 75–86.
Lakin, G.F., Biometriya: Ucheb. posobie dlya biol. spets. vuzov (Biometrics: Tutorial for Biologists of Specialized Universities), Moscow: Vysshaya Shkola, 1990.
Markovic, Z., Preiner, D., Stupić, D., Andabaka, Z.Š, Vončina, D., Maletić, E., Karoglan Kontić, J., Chatelet, P., and Engelmann, F., Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.), Vitis, 2015, vol. 54, pp. 247–251.
Nei, M., Genetic distance between populations, Am. Nat., 1972, vol. 106, pp. 283–292.
Nunes, C.F., Ferreira, J.L., Nunes-Fernandes, M.C., de Souza, BrevesS., Generoso, A.L., Fontes-Soares, B.D., Carvalho-Dias, M.S., Pasqual, M., Borem, A., and de Almeida Cancado, G.M., An improved method for genomic DNA extraction from strawberry leaves, Ciencia Rural, 2011, vol. 41, pp. 1383–1389.
Ries, G., Heller, W., Puchta, H., Sandermann, H., Seidlitz, H.K., and Hohn, B., High UV-B radiation reduces genome stability in plants, Nature, 2000, vol. 406, pp. 98–101.
Solov’eva, A.I., Dolgikh, Yu.I., Vysotskaya, O.N., and Popov, A.S., Patterns of ISSR and REMAP DNA markers after cryogenic preservation of spring wheat calluses by dehydration method, Russ. J. Plant Physiol., 2011, vol. 58, no. 3, pp. 423–430.
Solov’eva, A.I., Vysotskaya, O.N., and Dolgikh, Yu.I., Effect of dehydration duration of apices on characteristics of in vitro plants of Fragaria vesca after cryopreservation, Russ. J. Plant Physiol., 2016, vol. 63, no. 2, pp. 243–251.
De Verno, L.L., Park, Y.S., Bonga, J.M., and Barrett, J.D., Somaclonal variation in cryopreserved embryogenic clones of white spruce (Picea glauca (Moench) Voss.), Plant Cell Rep., 1999, vol. 18, pp. 948–953.
Vysotskaya, O.N., Danilova, S.A., and Popov, A.S., A method for cryopreservation in vitromeristems isolated from strawberry (Fragaria L.) plants, RF Patent No. 2302107, Byull. Izobret., 2007, no. 19.
Yao, Y. and Kovalchuk, I., Abiotic stress leads to somatic and heritable changes in homologous recombination frequency, point mutation frequency and microsatellite stability in Arabidopsis plants, Mutat. Res., 2011, vol. 707, pp. 61–66.
This work was supported by the Russian Foundation for Basic Research, project no. 14-04-31615.COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Translated by T. Borisova
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Solov’eva, A.I., Vysotskaya, O.N. Genetic Diversity of a Post-Cryogenic Fragaria vesca L. Clone after Long-Term Cultivation in vitro and Influenced by Abiotic Factors. Biol Bull Russ Acad Sci 47, 253–258 (2020). https://doi.org/10.1134/S1062359020030085