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Cytogenetic Activity of Gold Nanoparticles in Germ and Somatic Cells of 129 Mice with a Nonsense Mutation in the DNA Polymerase Iota Gene

  • Developmental Biology
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Abstract

In this study, we investigated the cytogenetic effects of single and quadruple exposure of spermatogenic cells and hepatocytes of 129 mice, which have a mutation in the gene that encodes DNA polymerase iota, to ultrasmall gold nanoparticles (GNPs). The combined effects of GNPs and chemical mutagen dipin were evaluated. In all cases, except for the experiment with the quadruple GNP injection, we observed a slight, statistically nonsignificant increase in the frequency of round spermatids with micronuclei compared to the negative control (saline). It is established that, in the intact liver of 129 mice, in all variants of the experiment, GNPs behaved as a potentially cytotoxic agent, as evidenced by the decrease in the frequency of the micronucleated hepatocytes.

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Authors and Affiliations

  1. Faculty of Biology, Moscow State University, Moscow, 119991, Russia

    N. M. Mudzhiri, S. T. Zakhidov & I. A. Zelenina

  2. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, 119071, Russia

    V. M. Rudoy & O. V. Dement’eva

  3. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    N. M. Mudzhiri & T. L. Marshak

  4. Higher School of Economics National Research University, Moscow, 101000, Russia

    A. A. Makarov

  5. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182, Russia

    I. V. Makarova & L. E. Andreeva

Authors
  1. N. M. Mudzhiri
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  2. S. T. Zakhidov
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  3. V. M. Rudoy
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  4. O. V. Dement’eva
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  5. A. A. Makarov
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  6. I. V. Makarova
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  7. I. A. Zelenina
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  8. L. E. Andreeva
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  9. T. L. Marshak
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Corresponding author

Correspondence to T. L. Marshak.

Additional information

Original Russian Text © N.M. Mudzhiri, S.T. Zakhidov, V.M. Rudoy, O.V. Dement’eva, A.A. Makarov, I.V. Makarova, I.A. Zelenina, L.E. Andreeva, T.L. Marshak, 2018, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2018, No. 2, pp. 137–143.

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Mudzhiri, N.M., Zakhidov, S.T., Rudoy, V.M. et al. Cytogenetic Activity of Gold Nanoparticles in Germ and Somatic Cells of 129 Mice with a Nonsense Mutation in the DNA Polymerase Iota Gene. Biol Bull Russ Acad Sci 45, 119–125 (2018). https://doi.org/10.1134/S1062359018020085

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  • DOI: https://doi.org/10.1134/S1062359018020085

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