Differential Scanning Calorimetry as a Method for the Control of Vegetable Oils


Differential scanning calorimetry (DSC) was used to study the thermophysical properties of oils of amaranth, corn, flax, sunflower, rapeseed, milk thistle, camelina, and pumpkin seed, liquid at room temperature. The characteristic thermal effects of these oils (temperatures of the maxima of endothermic peaks and their areas in the DSC thermograms) were determined. Endothermic peaks of different intensities on the melting curves of liquid vegetable oils in the ranges from –40 to –15°C, from –25 to –8°C, from –19 to +6°C, and from –10 to +4 °C as identification factors are discussed. The coordinates of the maxima of these peaks on the abscissa axis (Ti) and their areas (Si) significantly correlate with the concentrations of basic fatty acids and triacylglycerols (Wi, %), determined by reversed-phase HPLC. We demonstrated that the authenticity of vegetable oils could be effectively controlled by DSC.

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  1. 1

    Tan, C.P. and Cheman, Y.B., J. Am. Oil Chem. Soc., 2000, vol. 77, no. 2, p. 143.

    CAS  Article  Google Scholar 

  2. 2

    Tomaszewska-Gras, J., Food Control, 2016, vol. 60, no. 2, p. 629.

    CAS  Article  Google Scholar 

  3. 3

    Vereshchagin, A.L., Reznichenko, I.Yu., and Bychin, N.V., Tekh. Tekhnol. Pishch. Proizvod., 2019, vol. 49, no. 2, p. 289.

    Google Scholar 

  4. 4

    Rudakov, O.B., Saranov, I.A., and Polyanskii, K.K., Analitika Kontrol’, 2019, vol. 23, no. 1, p. 127.

    Google Scholar 

  5. 5

    Van Wetten, I.A., Van Herwaarden, A.W., Splinter, R., Boerrigter-Eenling, R., and Van Ruth, S.M., Thermochim. Acta, 2015, vol. 603.

  6. 6

    Chatziantoniou, S.E., Triantafillou, D.J., Karayannakidis, P.D., and Diamantopoulos, E., Thermochim. Acta, 2014, vol. 576, p. 9.

    CAS  Article  Google Scholar 

  7. 7

    Zhang Zhen-Shan, Li Dong, Zhang Li-Xia, Liu Yu-Lan, and Wang Xue-De, J. Therm. Anal. Calorim., 2014, vol. 117, no. 3, p. 2129.

    Article  Google Scholar 

  8. 8

    Rudakov, O.B., Ponomarev, A.N., Polyanskii, K.K., and Lyubar’, A.V., Zhiry. Khimicheskii sostav i ekspertiza kachestva (Fats. Chemical Composition and Quality Examination), Moscow: DeLi Print, 2005.

  9. 9

    O’Brien, R., Fat and Oils: Formulating and Processing for Applications, Boca Raton: CRC, 2008, 3rd ed.

    Google Scholar 

  10. 10

    Deineka, V.I., Turtygin, A.V., and Deineka, L.A., Analitika Kontrol’, 2016, vol. 20, no. 4, p. 314.

    Google Scholar 

  11. 11

    An, V.N., Deineka, V.I., Khien, Ch.T.N., Deineka, L.A., and Rudakov, O.B., Sorbtsionnye Khromatogr. Protsessy, 2018, vol. 18, no. 6, p. 816.

    Google Scholar 

  12. 12

    An Van Nguen, Deineka, V.I., Long Kuok Fam, Fyong Lan Doan, Deineka, L.A., An Tkhi Ngok Vu, and Tkhui Tkhi Tkhu Din, Khim. Rastit. Syr’ya, 2019, no. 3, p. 53.

  13. 13

    An Nguen Van, Popova, A.A., Deineka, V.I., and Deineka, L.A., J. Anal. Chem., 2017, vol. 72, no. 9, p. 1007.

  14. 14

    Deineka, V.I. Deineka, L.A., Anisimovich, I.P., Peristyi, V.A., and Turtygin, A.V., Zavod. Lab., Diagn. Mater., 2008, vol. 74, no. 3, p. 15.

    CAS  Google Scholar 

  15. 15

    Deineka, V.I., Staroverov, V.M., Fofanov, G.M., and Balyatinskaya, L.N., Pharm. Chem. J., 2002, vol. 36, no. 7, p. 392.

    CAS  Article  Google Scholar 

  16. 16

    Andrikopoulos, N.K., Crit. Rev. Food Sci. Nutr., 2002, vol. 42, no. 5, p. 473.

    CAS  Article  Google Scholar 

  17. 17

    Jabeur, H., Zribi, A., Makni, J., Rebai, A., Abdelhedi, R., and Bouaziz, M., J. Agric. Food Chem., 2014, vol. 62, no. 21, p. 4893.

    CAS  Article  Google Scholar 

  18. 18

    Podlaha, O. and Toregerd, B., J. High Resolut. Chromatogr. Chromatogr. Commun., 1982, vol. 5, p. 553.

    CAS  Article  Google Scholar 

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The authors are grateful to L.A. Miroshnichenko, PhD (Biol.) (Russkaya Oliva), for the provided samples of vegetable oils.


This work was supported by the Russian Science Foundation, project no. MK-590.2020.8.

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Correspondence to O. B. Rudakov or I. A. Saranov.

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Translated by O. Zhukova

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Rudakov, O.B., Saranov, I.A., Van, A.N. et al. Differential Scanning Calorimetry as a Method for the Control of Vegetable Oils. J Anal Chem 76, 258–266 (2021). https://doi.org/10.1134/S1061934821020118

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  • liquid vegetable oils
  • differential scanning calorimetry
  • melting curves
  • reversed-phase HPLC
  • triglyceride composition