Journal of Analytical Chemistry

, Volume 73, Issue 3, pp 283–291 | Cite as

Determination of the Origin of 19-Norandrosterone in Urine by Gas Chromatography–Isotope-Ratio Mass Spectrometry for Doping Control

  • I. I. Podolskiy
  • T. G. Sobolevskii
  • M. A. Dikunets


The most difficult task in antidoping control is the identification of compounds whose origin can be either endogenous or exogenous. This paper presents a procedure for determining the origin of 19-norandrosterone based on the extraction of target components from urine, their multistage purification by semipreparative HPLC, acetylation, and quantification by gas chromatography–isotope-ratio mass spectrometry and gas chromatography–mass spectrometry. Effects of isotope fractionation were not observed in sample preparation. The degree of extraction of the target analytes was 80 ± 5%.


doping anabolic steroids 19-norandrosterone gas chromatography–combustion–isotope-ratio mass spectrometry 


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  1. 1.
    Galimov, E.M., Sevast’yanov, V.S., Kul’bachevskaya, E.V., and Golyavin, A.A., Mass-Spektrom., 2004, vol. 1, no. 1, p.31.Google Scholar
  2. 2.
    Mosandl, A., Food Rev. Int., 1995, vol. 11, p.597.CrossRefGoogle Scholar
  3. 3.
    Woodbury, S.E., Evershed, R.P., and Rossel, J.B., J. Chromatogr. A, 1998, vol. 805, p.249.CrossRefGoogle Scholar
  4. 4.
    Dowling, L.M., Boreham, C.J., Hope, J.M., Murray, A.P., and Summons, R.E., Org. Geochem., 1995, vol. 23, p.729.CrossRefGoogle Scholar
  5. 5.
    Hofmann, D., Jung, K., Segschneider, H.-J., Gehre, M., and Schuurmann, G., Isot. Environ. Health Stud., 1995, vol. 31, p.367.CrossRefGoogle Scholar
  6. 6.
    Becchi, M., Aguilera, R., Farizon, Y., Flament, M., Casabianca, H., and James, P., Rapid Commun. Mass Spectrom., 1994, vol. 8, p.304.CrossRefGoogle Scholar
  7. 7.
    WADA Technical Document for Endogenous Anabolic Androgenic Steroids, TD2004EAAS, Montreal: World Anti-Doping Agency, 2004.Google Scholar
  8. 8.
    WADA Technical Document for the Detection of Synthetic Forms of Endogenous Anabolic Androgenic Steroids by GC–C–IRMS, TD2016IRMS, Montreal: World Anti-Doping Agency, 2016.Google Scholar
  9. 9.
    Schanzer, W., Clin. Chem., 1996, vol. 42, p. 1001.Google Scholar
  10. 10.
    Engel, L.L. and Wheeler, M., J. Biol. Chem., 1958, vol. 231, p.159.Google Scholar
  11. 11.
    Le Bizec, B. and Monteau, F., J. Chromatogr. B: Biomed. Sci. Appl., 1999, vol. 723, p.157.CrossRefGoogle Scholar
  12. 12.
    Saudan, C., Baume, N., and Mangin, P., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2004, vol. 810, p.157.CrossRefGoogle Scholar
  13. 13.
    Trout, G.J., Rogerson, J.H., Cawley, A.T., and Alma, C.W., Aust. J. Chem., 2003, vol. 56, p.175.CrossRefGoogle Scholar
  14. 14.
    Grosse, J. and Anielski, P., Steroids, 2005, vol. 70, p.499.CrossRefGoogle Scholar
  15. 15.
    Mareck-Engelke, U. and Schultze, G., Recent Adv. Doping Anal., 2000, vol. 8, p.145.Google Scholar
  16. 16.
    Hemmersbach, P., Hagensen, A.H., and Misund, J., Recent Adv. Doping Anal., 2000, vol. 8, p.141.Google Scholar
  17. 17.
    WADA Technical Document for the Harmonization of Analysis and Reporting of 19-Norsteroids Related to Nandrolone, TD2016NA, Montreal: World Anti-Doping Agency, 2016.Google Scholar
  18. 18.
    Sobolevsky, T., Krotov, G., Dikunets, M., Nikitina,M., Mochalova, E., and Rodchenkov, G., Drug Test. Anal., 2014, vol. 6, p. 1087.CrossRefGoogle Scholar
  19. 19.
    Piper, T., Buisson, C., and de la Torre, X., GC–C–IRMS Technical Note, Montreal: World Anti-Doping Agency, 2016.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • I. I. Podolskiy
    • 1
  • T. G. Sobolevskii
    • 2
  • M. A. Dikunets
    • 1
  1. 1.Antidoping CentreMoscowRussia
  2. 2.UCLA Olympic Analytical LaboratoryLos AngelesUSA

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