Journal of Analytical Chemistry

, Volume 73, Issue 3, pp 283–291 | Cite as

Determination of the Origin of 19-Norandrosterone in Urine by Gas Chromatography–Isotope-Ratio Mass Spectrometry for Doping Control

  • I. I. Podolskiy
  • T. G. Sobolevskii
  • M. A. Dikunets
Articles

Abstract

The most difficult task in antidoping control is the identification of compounds whose origin can be either endogenous or exogenous. This paper presents a procedure for determining the origin of 19-norandrosterone based on the extraction of target components from urine, their multistage purification by semipreparative HPLC, acetylation, and quantification by gas chromatography–isotope-ratio mass spectrometry and gas chromatography–mass spectrometry. Effects of isotope fractionation were not observed in sample preparation. The degree of extraction of the target analytes was 80 ± 5%.

Keywords

doping anabolic steroids 19-norandrosterone gas chromatography–combustion–isotope-ratio mass spectrometry 

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • I. I. Podolskiy
    • 1
  • T. G. Sobolevskii
    • 2
  • M. A. Dikunets
    • 1
  1. 1.Antidoping CentreMoscowRussia
  2. 2.UCLA Olympic Analytical LaboratoryLos AngelesUSA

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