Journal of Analytical Chemistry

, Volume 72, Issue 7, pp 751–755 | Cite as

Chemiliminescence determination of lipid hydroperoxides in biological fluids

  • E. V. Proskurnina
  • A. A. Dzhatdoeva
  • E. N. Lobichenko
  • R. I. Shalina
  • Yu. A. Vladimirov


To evaluate the oxidative stress under clinical conditions, a procedure is developed for the chemiluminescence determination of the total concentration of lipid hydroperoxides based on their oxidation in the presence of microperoxidase and chemiluminescence activator isoluminol in a borate buffer solution (pH 10.0). The limit of detection for linoleic acid hydroperoxide is 16 nM. The procedure is used to determine lipid hydroperoxides in follicular fluid lipoproteins and in plasma of patients undergoing extracorporeal fertilization.


oxidative stress lipid peroxidation activated chemiluminescence microperoxidase plasma lipoproteins follicular fluid lipoproteins 


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  1. 1.
    Lushchak, V.I. and Gospodaryov, D.V., Oxidative Stress and Diseases, Rijeka: InTech, 2012.CrossRefGoogle Scholar
  2. 2.
    Li, W.G., Stoll, L.L., Rice, J.B., Xu, S.P., Miller, F.J., Jr., Chatterjee, P., Hu, L., Oberley, L.W., Spector, A.A., and Weintraub, N.L., Free Rad. Biol. Med., 2003, vol. 34, p. 937.CrossRefGoogle Scholar
  3. 3.
    Miyamoto, S. and Di Mascio, P., Subcell. Biochem., 2014, vol. 77, p. 3.CrossRefGoogle Scholar
  4. 4.
    Compton, C.N., Franko, A.P., Murray, M.T., Diebel, L.N., and Dulchavsky, S.A., J. Trauma, 1998, vol. 44, p. 783.CrossRefGoogle Scholar
  5. 5.
    Berliner, J.A. and Heinecke, J.W., Free Rad. Biol. Med., 1996, vol. 20, p. 707.CrossRefGoogle Scholar
  6. 6.
    Zorn-Pauly, K., Schaffer, P., Pelzmann, B., Bernhart, E., Wei, G., Lang, P., Ledinski, G., Greilberger, J., Koidl, B., and Jurgens, G., Cardiovasc. Res., 2005, vol. 66, p. 74.CrossRefGoogle Scholar
  7. 7.
    Asakawa, T.M., J. Am. Oil Chem. Soc., 1978, vol. 55, p. 619.CrossRefGoogle Scholar
  8. 8.
    Hara, S. and Totani, Y., J. Am. Oil Chem. Soc., 1988, vol. 65, p. 1948.CrossRefGoogle Scholar
  9. 9.
    Dobarganes, M.C. and Velasco, J., Eur. J. Lipid Sci. Tech., 2002, vol. 104, p. 420.CrossRefGoogle Scholar
  10. 10.
    Ma, K., van de Voort, F.R., Sedman, J., and Ismail, A.A., J. Am. Oil Chem. Soc., 1997, vol. 74, p. 897.CrossRefGoogle Scholar
  11. 11.
    Yamaguchi, Y., Kagota, S., Kunitomo, M., and Haginaka, J., J. Chromatogr. B: Biomed. Sci. Appl., 1999, vol. 731, p. 223.CrossRefGoogle Scholar
  12. 12.
    Belghmi, K., Nicolas, J.C., and Crastes de Paulet, A., J. Biolumin. Chemilumin., 1988, vol. 2, p. 113.CrossRefGoogle Scholar
  13. 13.
    Driomina, E., Polnikov, I., Sharov, V., Azizova, O., and Vladimirov, Y., Free Rad. Res., 1994, vol. 20, p. 279.CrossRefGoogle Scholar
  14. 14.
    Volkova, P.O., Alekseev, A.V., Dzhatdoeva, A.A., Proskurnina, E.V., and Vladimirov, Yu.A., Moscow Univ. Chem. Bull., 2016, vol. 71, no. 1, p. 87.CrossRefGoogle Scholar
  15. 15.
    Iwaoka, T. and Tabata, F., FEBS Lett., 1984, vol. 178, p. 47.CrossRefGoogle Scholar
  16. 16.
    Teselkin, Yu.O. and Babenkova, I.V., Vestn. Ross. Gos. Med. Univ., 2011, no. 1, p. 54.Google Scholar
  17. 17.
    Jana, S.K., Chattopadhyay, N.B., Chakravarty, B., and Chaudhury, K., Reprod. Toxicol., 2010, vol. 29, p. 447.CrossRefGoogle Scholar
  18. 18.
    Pasqualotto, E.B., Agarwal, A., Sharma, R.K., Izzo, V.M., Pinotti, J.A., Joshi, N.J., and Rose, B.I., Fertil. Steril., 2004, vol. 81, p. 973.CrossRefGoogle Scholar
  19. 19.
    Vladimirov, Y.A. and Sherstnev, M.P., Sov. Med. Rev. B. Physicochem. Asp. Med., 1991, vol. 2, p. 1.Google Scholar
  20. 20.
    Ibusuki, D., Nakagawa, K., Asai, A., Oikawa, S., Masuda, Y., Suzuki, T., and Miyazawa, T., J. Lipid Res., 2008, vol. 49, p. 2668.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • E. V. Proskurnina
    • 1
  • A. A. Dzhatdoeva
    • 1
  • E. N. Lobichenko
    • 1
  • R. I. Shalina
    • 2
  • Yu. A. Vladimirov
    • 1
  1. 1.Department of Fundamental MedicineMoscow State UniversityMoscowRussia
  2. 2.Department of PediatricsPirogov Russian National Research Medical UniversityMoscowRussia

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