Journal of Analytical Chemistry

, Volume 72, Issue 3, pp 309–315 | Cite as

Detection and quantification of chitosan aggregates by pressure-assisted capillary zone electrophoresis

  • T. G. Dzherayan
  • N. G. Vanifatova
  • A. A. Burmistrov
  • E. V. Lazareva
  • A. V. Rudnev


An approach to the detection and determination of chitosan aggregates in acetic acid solutions is proposed using pressure-assisted capillary zone electrophoresis. Processes of chitosan aggregation are studied depending on the composition of dispersion medium and storage time. The presence of several species of positively charged chitosan aggregates is revealed for the first time. Particle sizes in the range 20–2500 nm are determined by scanning electron microscopy and static and dynamic light scattering. The dependence of the shape of electropherograms on particle size distribution obtained under the same conditions is found. A trend to changing electrophoretic mobility depending on the size of the aggregate is observed, which enables the approximate evaluation of the polydispersity of chitosan solutions. Chitosan is used for the effective dynamic modification of capillaries, which does not require the introduction of a modifier into the background electrolyte.


chitosan capillary zone electrophoresis static light scattering dynamic light scattering scanning electron microscopy 


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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • T. G. Dzherayan
    • 1
  • N. G. Vanifatova
    • 1
  • A. A. Burmistrov
    • 1
  • E. V. Lazareva
    • 2
  • A. V. Rudnev
    • 1
  1. 1.Vernadsky Institute of Geochemistry and Analytical ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of ChemistryMoscow State UniversityMoscowRussia

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