Journal of Analytical Chemistry

, Volume 66, Issue 14, pp 1353–1360 | Cite as

Mass spectral study of the skin peptide of brown frog Rana temporaria from Zvenigorod population

  • T. Yu. Samgina
  • V. A. Gorshkov
  • Ye. A. Vorontsov
  • K. A. Artemenko
  • S. V. Ogourtsov
  • R. A. Zubarev
  • A. T. Lebedev
Articles

Abstract

Skin secretions of amphibian are an interesting source of biologically active peptides. The present study provides the profile of the skin secretions of the brown frog Rana temporaria from Zvenigorod population (Russia). Sequencing of the skin secretion components has been carried out on an ion cyclotron resonance instrument with electrospray ionization and two methods of fragmentation activation, collisional activation and electron capture. For sequencing of the peptides containing intermolecular C-terminal disulfide cycle two methods of disulfide bond opening have been used: reduction with subsequent alkylation of the free thiol groups and oxidation with performic acid with the formation of sulfo-acid groups. The peptide profile of Rana temporaria studied by a complex mass spectral method has been compared with the data for the frogs of other European populations of this species. For the first time we have revealed ornithokinin-antagonist of the ornithokinin receptor-in skin secretions of amphibians.

Keywords

Rana temporaria Fourier transformation ion cyclotron resonance mass spectral sequencing disulfide-containing peptides ornithokinin temporins 

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • T. Yu. Samgina
    • 1
  • V. A. Gorshkov
    • 1
  • Ye. A. Vorontsov
    • 1
  • K. A. Artemenko
    • 2
  • S. V. Ogourtsov
    • 3
  • R. A. Zubarev
    • 4
  • A. T. Lebedev
    • 1
  1. 1.Department of ChemistryMoscow State UniversityMoscowRussia
  2. 2.Department of Physical and Analytical ChemistryUppsala UniversityUppsalaSweden
  3. 3.Department of BiologyMoscow State UniversityMoscowRussia
  4. 4.Department of Medicinal Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden

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