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Russian Journal of Genetics

, Volume 54, Issue 3, pp 322–327 | Cite as

Sequence of the mrjp3 Microsatellite Locus in Honeybees of Different Origin

  • N. V. Ostroverkhova
  • A. N. Kucher
  • N. P. Babushkina
  • O. L. Konusova
Animal Genetics

Abstract

The sequencing of the nucleotide sequences of the mrjp3 repetitive region (mrjp3 microsatellite locus) in Siberian honeybees was carried out. A high similarity of the studied nucleotide sequences (≥99% identity) with reference sequences was observed, which indicates a high conservation of the mrjp3 repetitive region in different Apis mellifera subspecies.

Keywords

honeybee mrjp3 microsatellite locus evolutionary branches M and C Apis mellifera mellifera L. Apis mellifera carpatica Avet. Siberia 

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References

  1. 1.
    Albert, S., Klaudiny, J., and Simúth, J., Molecular characterization of MRJP3, highly polymorphic protein of honeybee (Apis mellifera) royal jelly, Insect Biochem. Mol. Biol., 1999, vol. 29, no. 5, pp. 427–434.CrossRefPubMedGoogle Scholar
  2. 2.
    Albert, S., and Simúth, J., Characterization of major royal jelly protein-like DNA sequences in Apis dorsata, J. Apicult. Res., 2002, vol. 41, nos. 3–4, pp. 75–82. doi 10.1080/00218839.2002.11101072CrossRefGoogle Scholar
  3. 3.
    Albertová, V., Su, S., Brockmann, A., et al., Organization and potential function of the mrjp3 locus in four honeybee species, J. Agric. Food Chem., 2005, vol. 53, no. 20, pp. 8075–8081. doi 10.1021/jf051417xCrossRefPubMedGoogle Scholar
  4. 4.
    Buttstedt, A., Moritz, R.F., and Erler, S., More than royal food—Major royal jelly protein genes in sexuals and workers of the honeybee Apis mellifera, Front. Zool., 2013, vol. 10, no. 72.Google Scholar
  5. 5.
    Drapeau, M.D., Albert, S., Kucharski, R., et al., Evolution of the Yellow/Major royal jelly protein family and the emergence of social behavior in honey bees, Genome Res., 2006, vol. 16, pp. 1385–1394.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Buttstedt, A., Moritz, R.F., and Erler, S., Origin and function of the major royal jelly proteins of the honeybee (Apis mellifera) as members of the yellow gene family, Biol. Rev., 2014, vol. 89, no. 2, pp. 255–269. doi 10.1111/brv.12052CrossRefPubMedGoogle Scholar
  7. 7.
    Albert, S., Bhattacharya, D., Klaudiny, J., et al., The family of major royal jelly proteins and its evolution, J. Mol. Evol., 1999, vol. 49, no. 2, pp. 290–297.CrossRefPubMedGoogle Scholar
  8. 8.
    Schmitzová, J., Klaudiny, J., Albert, S., et al., A family of major royal jelly proteins of the honeybee Apis mellifera L., Cell. Mol. Life Sci., 1998, vol. 54, no. 9, pp. 1020–1030.CrossRefPubMedGoogle Scholar
  9. 9.
    Albert, S. and Klaudiny, J., The MRJP/YELLOW protein family of Apis mellifera: identification of new members in the EST library, J. Insect Physiol., 2004, vol. 50, no. 1, pp. 51–59.CrossRefPubMedGoogle Scholar
  10. 10.
    Baitala, T.V., Faquinello, P., Toledo, V.A.A., et al., Potential use of major royal jelly proteins (MRJPs) as molecular markers for royal jelly production in Africanized honeybee colonies, Apidologie, 2010, vol. 41, pp. 160–168. doi 10.1051/apido/2009069CrossRefGoogle Scholar
  11. 11.
    Parpinelli, R.S., Ruvolo-Takasusuki, M.C.C., and Toledo, V.A.A., MRJP microsatellite markers in Africanized Apis mellifera colonies selected on the basis of royal jelly production, Genet. Mol. Res., 2014, vol. 13, no. 3, pp. 6724–6733.CrossRefPubMedGoogle Scholar
  12. 12.
    Ruvolo-Takasusuki M.C.C., Pozza A.P.B.C., Oliveira A.P.N.Z., et al., Improvement and selection of honeybees assisted by molecular markers, Beekeeping and Bee Conservation—Advances in Research, Dechechi Chambo, E., Ed., InTech, Croatia, 2016, pp. 63–75. doi 10.5772/62426.10.5772/62426Google Scholar
  13. 13.
    Ji, T., Liu, Z., Shen, J., et al., Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann, BMC Genomics, 2014, vol. 15, no. 665. pmid 25103401. doi 10.1186/1471-2164-15-665Google Scholar
  14. 14.
    Ostroverkhova, N.V., Konusova, O.L., Kucher, A.N., et al., Population genetic structure of honeybee (Apis mellifera L.) near the village of Leboter, Chainskii raion, Tomsk oblast, Vestn. Tomsk. Gos. Univ., Biol., 2013, no. 1 (21), pp. 161–172.Google Scholar
  15. 15.
    Ostroverkhova, N.V., Konusova, O.L., Kucher, A.N., et al., Genetic diversity of the locus COI-COII of mitochondrial DNA in honeybee populations (Apis mellifera L.) from the Tomsk region, Russ. J. Genet., 2015, vol. 51, no. 1, pp. 80–90. doi 10.1134/S102279541501010XCrossRefGoogle Scholar
  16. 16.
    Konusova, O.L., Ostroverkhova, N.V., Kucher, A.N., et al., Morphometric variability of honeybees Apis mellifera L., differing in the variants of the mtDNA COICOII locus, Vestn. Tomsk. Gos. Univ., Biol., 2016, no. 1 (33), pp. 62–81. doi 10.17223/19988591/33/5CrossRefGoogle Scholar
  17. 17.
    Ostroverkhova, N.V., Konusova, O.L., Kucher, A.N., and Sharakhov, I.V., A comprehensive characterization of the honeybees in Siberia (Russia), in Beekeeping and Bee Conservation—Advances in Research, Dechechi Chambo, E., Ed., InTech, Croatia, 2016, pp. 1–37. doi 10.5772/62395Google Scholar
  18. 18.
    Ostroverkhova, G.P., Konusova, O.L., and Pogorelov, Yu.L., Biologicheskaya i khozyaistvennaya otsenka pchelinoi sem’i (Apis mellifera L.) (Biological and Business Assessment of Honey Bee (Apis mellifera L.) Family), Tomsk: NTL, 2005.Google Scholar
  19. 19.
    Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symp., 1999, vol. 41, pp. 95–98.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • N. V. Ostroverkhova
    • 1
  • A. N. Kucher
    • 1
    • 2
  • N. P. Babushkina
    • 2
  • O. L. Konusova
    • 1
  1. 1.National Research Tomsk State UniversityTomskRussia
  2. 2.Research Institute of Medical GeneticsTomsk National Research Medical CenterTomskRussia

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