Russian Journal of Genetics

, Volume 54, Issue 3, pp 305–313 | Cite as

Composition of High Molecular Weight Glutenin Subunits in Common Wheat Varieties and Promising Lines

  • L. V. Obukhova
  • V. K. Shumny
Plant Genetics


The composition of high molecular weight glutenin subunits (HMWGS) is studied by means of the SDS-PAGE electrophoresis method in varieties of winter (obtained with the involvement of the Agropyron glaucum (Desf.)) and spring common wheat created in the West Siberian region. Data on HMWGS in the promising lines obtained with the involvement of the Triticum timopheevii Zhuk. and T. dicoccum var. farrum (having a complex resistance to pathogens) are presented. The prospects of the creation of spring common wheat varieties with the involvement of the presented lines and the use of some of them as a model for the study of heterosis mechanisms are discussed.


high molecular weight glutenin subunits winter wheat spring wheat introgressive lines Agropyron glaucum (Desf.) Triticum timopheevii Zhuk. Triticum dicoccum var. farrum 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Koehle, P. and Wieser, H., Chemistry of cereal grains, in Handbook on Sourdough Biotechnology, Gobbetii, M. and Ganzle, M., Eds., 2013, vol. 6, pp. 11–46.CrossRefGoogle Scholar
  2. 2.
    Wieser, H., Bushuk, W., and MacRitchie, F., The polymeric glutenins, in Gliadin and Glutenin: The Unique Balance of Wheat Quality, Wrigley, C., Bekes, F., and Bushuk, W., Eds., St. Paul: AACC International, 2006, pp. 213–240.CrossRefGoogle Scholar
  3. 3.
    Payne, P.I. and Lawrence, G.J., Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat, Cereal Res. Comm., 1983, vol. 11, pp. 29–35.Google Scholar
  4. 4.
    Payne, P.I., Nightingale, M.A., Krattiger, A.F., and Holt, L.M., The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties, J. Sci. Food Agric., 1987, vol. 40, pp. 51–65.CrossRefGoogle Scholar
  5. 5.
    Payne, P.I., Holt, L.M., Krattiger, A.F., and Carrillo, J.M., Relationships between seed quality characteristics and HMW glutenin subunit composition determined using wheat grown in Spain, J. Cereal Sci., 1988, vol. 7, pp. 229–235.CrossRefGoogle Scholar
  6. 6.
    Branlard, G. and Dardevet, M., Diversity of grain protein and bread wheat quality: 2. Correlation between high molecular weight subunits of glutenin and flour quality characteristics, J. Cereal Sci., 1985, vol. 3, pp. 345–354.CrossRefGoogle Scholar
  7. 7.
    Eagles, H.A., Hollamby, G.J., and Eastwood, R.F., Genetic and environmental variation for grain quality traits routinely evaluated in southern Australian wheat breeding programs, Austral. J. Agric. Res., 2002, vol. 53, pp. 1047–1057.CrossRefGoogle Scholar
  8. 8.
    Liu, L., He, Z.H., Yan, J., et al., Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats, Euphytica, 2005, vol. 142, pp. 197–204.CrossRefGoogle Scholar
  9. 9.
    Morgunov, A.I., Rogers, W.J., Sayers, E.J., and Metakovsky, E.V., The high-molecular weight glutenin subunits composition of Soviet wheat varieties, Euphytica, 1990, vol. 51, pp. 41–52.CrossRefGoogle Scholar
  10. 10.
    Nettevich, E.D., Morgunov, A.I., Rogers, D.U., et al., Characterization of Russian wheat varieties by high molecular weight glutenin subunits, Dokl. Vses. Akad. S.-kh. Nauk, im. V.I. Lenina, 1991, no. 7, pp. 2–5.Google Scholar
  11. 11.
    Payne, P.I., Genetics of wheat storage proteins and effect of allele variation on bread-making quality, Ann. Rev. Physiol., 1987, vol. 38, pp. 141–153.CrossRefGoogle Scholar
  12. 12.
    McIntosh, R.A., Dubcovsky, J., Rogers, J., et al., Catalogue of Gene Symbols for Wheat, 2013. jsp.Google Scholar
  13. 13.
    Chekurov, V.M. and Orlova, A.M., Isolation of the homozygous lines of Agropyron glaucum for crossing with common wheat, S.-kh. Biol., 1982, vol. 17, no. 1, pp. 55–61.Google Scholar
  14. 14.
    Chekurov, V.M. and Sergeeva, S.I., The importance of parental components in the cross of soft wheat with Agropyron glaucum (Desf.), Genetika (Moscow), 1976, vol. 12, no. 3, pp. 153–155.Google Scholar
  15. 15.
    Chekurov, V.M., Kozlov, V.E., Titkov, I.P., and Mitrofanov, M.G., Problems and methodological approaches to the creation of winter wheat varieties for Siberia, in Geneticheskie metody v selektsii rastenii (Genetic Methods in Plant Breeding), Novosibirsk: Nauka, 1992, pp. 180–210.Google Scholar
  16. 16.
    Obukhova, L.V., Generalova, G.V., Maystrenko, O.I., et al., High-molecular-weight glutenin subunits in common wheat cultivars grown in the Novosibirsk region, in European Wheat Aneuploidy Co-Operative Newsletter, Pshenichnikova, T.A. and Worland, A.J., Eds., Norwich, 2001, pp. 143–146.Google Scholar
  17. 17.
    Obukhova, L.V., The glutenin of common wheat, Developing the Scientific Heritage of N.I. Vavilov at the Modern Stage (Proc. Int. Res. Conf. Dedicated to the 120th Birthday of Academician N.I. Vavilov), Kashevarov, N.I. and Khristov, Yu.A., Eds., Novosibirsk: Russ. Acad. Agricult. Sci., Sib. Branch, 2009.Google Scholar
  18. 18.
    Obukhova, L.V. and Budashkina, E.B., Storage proteins analysis of prolamins common wheat introgression lines with leaf rust resistance from Triticum timopheevii Zhuk., in European Wheat Aneuploidy Co-Operative Newsletter, Boerner, A. and Snape, J.W., Eds., Germany, Gatersleben, 2008, pp. 165–169.Google Scholar
  19. 19.
    Obukhova, L.V., Budashkina, E.B., and Shumny, V.K., Biochemical marker-assisted development of new common wheat line with HMW-glutenin genes from Triticum timopheevii Zhuk., in European Cereals Genetics Co-Operative Newsletter, Boerner, A. and Kobijlski, B., Eds., Serbia: Novi Sad, 2012, pp. 163–168.Google Scholar
  20. 20.
    Obukhova, L.V., Budashkina, E.B., Ermakova, M.F., et al., Grain and flour quality in the introgression lines of spring common wheat with the leaf rust resistance genes from Triticum timopheevii Zhuk., S.-kh. Biol., 2008, no. 5, pp. 38–42.Google Scholar
  21. 21.
    Budashkina E.B., Cytogenetic analysis of wheat interspecific hybrids (Triticum aestivum × Triticum dicoccum) and their breeding value, Cand. Sci. (Biol.) Dissertation, Novosibirsk: Inst. Tsitol. Genet. Sib. Otd. Ros. Akad. Nauk, 1975.Google Scholar
  22. 22.
    Dunduk, I.G., Budashkina, E.B., and Ermakova, M.F., Interspecific hybridization as a method of obtaining strong wheat, Sib. Vestn. S.-kh. Nauki, 1977, no. 4, pp. 25–30.Google Scholar
  23. 23.
    Budashkina E.B. and Kalinina, N.P., RF Patent 2138155, 1998.Google Scholar
  24. 24.
    Obukhova, L.V., Efremova, T.T., and Shumny, V.K., Characterization of puroindolines inthe control of endosperm texture in common wheat lines with substitutions of homeologous group-5 chromosomes, Russ. J. Genet., 2013, vol. 49, no. 3, pp. 294–298.CrossRefGoogle Scholar
  25. 25.
    Obukhova, L.V., Laikova, L.I., and Shumny, V.K., Analysis of storage proteins (prolamines, puroindolines and Waxy) in common wheat lines Triticum aestivum L. × (Triticum timopheevii Zhuk. × Triticum tauschii) with complex resistance to fungal infection, Russ. J. Genet., 2010, vol. 46, no. 6, pp. 672–676.CrossRefGoogle Scholar
  26. 26.
    Novoselskaya-Dragovich, A.Yu., Bespalova, L.A., Shishkina, A.A., et al., Genetic diversity of common wheat varieties at the gliadin-coding loci, Russ. J. Genet., 2015, vol. 51, no. 3, pp. 262–271.CrossRefGoogle Scholar
  27. 27.
    Kharakteristika sortov rastenii, vpervye vklyuchennykh v 2014 g. v Gosudarstvennyi reestr selektsionnykh dostizhenii, dopushchennykh k ispol’zovaniyu (Characterization of Plant Varieties First Included in 2014 into State Register of Selection Achievements Allowed for Use), Moscow: Min. Sel’sk. Khoz. RF, 2014.Google Scholar
  28. 28.
    Lubnin, A.N. and Sovetov, V.V., The results of spring common wheat improvement by breeding, in Aktual’nye zadachi selektsii i semenovodstva sel’skokhozyastvennykh rastenii na sovremennom etape (Current Issues of Breeding and Seed Production of Agricultural Plants in Recent Times), Novosibirsk: Ros. Akad. S.- Kh. Nauk, Sib. Otd., 2005, pp. 128–136.Google Scholar
  29. 29.
    Peng, Y., Yu, K., Zhang, Y., et al., Two novel y-type high molecular weight glutenin genes in Chinese wheat landraces of the Yangtze-River region, PLoS One, 2015, vol. 10, no. 11. e0142348. doi 10.1371/journal. pone.0142348CrossRefPubMedPubMedCentralGoogle Scholar
  30. 30.
    Abugalieva, A.I., Morgunov, A.I., Pena, R.H., et al., Kazakhstan—Siberian spring common wheat identification according to glutenin and gliadin composition. Vavilov J. Genet. and Breeding., 2015, vol. 19, no. 1, pp. 74–82.CrossRefGoogle Scholar
  31. 31.
    Abugalieva, A.I., Morgunov, A.I., Pena, R.H., et al., Identification of spring wheat genotypes by glutenin and gliadin subunit composition within the Kazakhstan—Siberia network of nurseries, Russ. J. Genet.: Appl. Res., 2016, vol. 6, no. 1, pp. 44–53. Scholar
  32. 32.
    Metakovskii, E.V., Koval’, S.F., and Sozinov, A.A., Stability and microevolution of the heterogeneous variety Saratovskaya 29, Vestn. S.-kh. Nauki, 1987, no. 9, pp. 28–34.Google Scholar
  33. 33.
    Barak, S., Mudgil, D., and Khatkar, B.S., Biochemical and functional properties of wheat gliadins: a review, Crit. Rev. Food Sci. Nutr., 2015, vol. 55, pp. 357–368.CrossRefPubMedGoogle Scholar
  34. 34.
    Gilbert, S.M., Wellner, N., Belton, P.S., et al., Expression and characterization of a highly repetitive peptide derived from a wheat seed storage protein, Acta Biochim. Biophys., 2000, vol. 1479, pp. 135–146.CrossRefGoogle Scholar
  35. 35.
    Obukhova, L.V. and Shumny, V.K., The inheritance of endosperm storage proteins by the line of the Saratovskaya 29 cultivar of common wheat from its parental forms, Russ. J. Genet., 2016, vol. 52, no. 1, pp. 49–55.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  1. 1.Federal Research Center Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia

Personalised recommendations