Advertisement

Russian Journal of Genetics

, Volume 54, Issue 3, pp 363–365 | Cite as

Effect of Heat Stress on Expression of DILP2 and DILP3 Insulin-Like Peptide Genes in Drosophila melanogaster Adults

  • O. V. Andreenkova
  • M. A. Eremina
  • N. E. Gruntenko
  • I. Yu. Rauschenbach
Short Communications
  • 20 Downloads

Abstract

In a number of works, it was demonstrated that insulin/insulin-like growth factor signaling pathway in the Drosophila melanogaster can be involved in the control of the organism reaction to stress. However, it remains unclear which of eight insulin-like peptides (ligands of insulin/insulin-like growth factor signaling pathway) known in the D. melanogaster are involved in the response to different types of stress. We conducted immunohistochemical analysis of the expression of two insulin-like peptide genes (DILP2 and DILP3) in insulin-producing cells of the brain in adult D. melanogaster females after heat stress. We for the first time found that the DILP3 is one of the components of the response to heat stress, while the DILP2 is apparently not involved in the organism response to heat stress.

Keywords

DILP2 and DILP3 insulin-like peptides insulin-producing cells heat stress Drosophila 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Toivonen, J.M. and Partridge, L., Endocrine regulation of aging and reproduction in Drosophila, Mol. Cell. Endocrinol., 2009, vol. 299, pp. 39–50.CrossRefPubMedGoogle Scholar
  2. 2.
    Nässel, D.R., Liu, Y., and Luo, J., Insulin/IGF signaling and its regulation in Drosophila, Gen. Comp. Endocrinol., 2015, vol. 221, pp. 255–266.CrossRefPubMedGoogle Scholar
  3. 3.
    Grönke, S., Clarke, D.F., Broughton, S., et al., Molecular evolution and functional characterization of Drosophila insulin-like peptides, PLoS Genet., 2010, vol. 6. e1000857CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Brogiolo, W., Stocker, H., and Ikeya, T., An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control, Curr. Biol., 2001, vol. 11, no. 4, pp. 213–221.CrossRefPubMedGoogle Scholar
  5. 5.
    Garelli, A., Gontijo, A.M., Miguela, V., et al., Imaginal discs secrete insulin-like peptide 8 to mediate plasticity of growth and maturation, Science, 2012, vol. 336, pp. 579–582.CrossRefPubMedGoogle Scholar
  6. 6.
    Colombani, J., Andersen, D.S., and Leopold, P., Secreted peptide Dilp8 coordinates Drosophila tissue growth with developmental timing, Science, 2012, vol. 336, pp. 582–585.CrossRefPubMedGoogle Scholar
  7. 7.
    Ikeya, T., Galic, M., Belawat, P., et al., Nutrientdependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila, Curr. Biol., 2002, vol. 12, pp. 1293–1300.CrossRefPubMedGoogle Scholar
  8. 8.
    Liu, Y., Liao, S., Veenstra, J.A., and Nässel, D.R., Drosophila insulin-like peptide 1 (DILP1) is transiently expressed during non-feeding stages and reproductive dormancy, Sci. Rep., 2016, vol. 6, p. 26620.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Cao, C. and Brown, M.R., Localization of an insulinlike peptide in brains of two flies, Cell Tissue Res., 2001, vol. 304, pp. 317–321.CrossRefPubMedGoogle Scholar
  10. 10.
    Veenstra, J.A., Agricola, H.-J., and Sellami, A., Regulatory peptides in fruit fly midgut, Cell Tissue Res., 2008, vol. 334, pp. 499–516.CrossRefPubMedGoogle Scholar
  11. 11.
    Broughton, S.J., Piper, M.D.W., Ikeya, T., et al., Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands, Proc. Natl. Acad. Sci. U.S.A., 2005, vol. 102, no. 8, pp. 3105–3110.CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Luo, J., Lushchak, O.V., Goergen, P., et al., Drosophila insulin-producing cells are differentially modulated by serotonin and octopamine receptors and affect social behavior, PLoS One, 2014, vol. 9, no. 6. e99732CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Gruntenko, N.E. and Rauschenbach, I.Yu., Interplay of juvenile hormone, 20-hydroxyecdisone and biogenic amines under normal and stress conditions and its effect on reproduction, J. Insect Physiol., 2008, vol. 56, pp. 902–908.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • O. V. Andreenkova
    • 1
  • M. A. Eremina
    • 1
  • N. E. Gruntenko
    • 1
  • I. Yu. Rauschenbach
    • 1
  1. 1.Federal Research Center Institute of Cytology and Genetics, Siberian BranchRussian Academy of SciencesNovosibirskRussia

Personalised recommendations