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Assessment of genomic imprinting of PPP1R9A, NAP1L5 and PEG3 in pigs

  • Animal Genetics
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Abstract

Imprinted genes play significant roles in the regulation of fetal growth and development, function of the placenta, and maternal nurturing behaviour in mammals. At present, few imprinted genes have been reported in pigs compared to human and mouse. In order to increase understanding of imprinted genes in swine, a polymorphism-based approach was used to assess the imprinting status of three porcine genes in 12 tissue types, obtained from F1 pigs of reciprocal crosses between Rongchang and Landrace pure breeds. In contrast to human and mouse homologues, porcine PPP1R9A was not imprinted, and was found to be expressed in all tissues examined. The expression of porcine NAP1L5 was detected in pituitary, liver, spleen, lung, kiduey, stomach, small intestine, skeletal muscle, fat, ovary, and uterus, but undetectable in heart. Furthermore, porcine NAP1L5 was paternally expressed in the tissues where it’s expression was observed. For PEG3, pigs expressed the paternal allele in skeletal muscle, liver, spleen, kidney, and uterus, but biallele in heart, lung, fat, stomach, small intestine, and ovary. Our data indicate that tissue distribution of the three gene differs among mammals, and the imprinting of NAP1L5 and PEG3 is well conserved.

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References

  1. Li, L., Keverne, E.B., Aparicio, S.A., et al., Regulation of Maternal Behavior and Offspring Growth by Paternally Expressed Peg3, Science, 1999, vol. 284, pp. 330–333.

    Article  PubMed  CAS  Google Scholar 

  2. Reik, W., Santos, F., and Dean, W., Mammalian epigenomics: Reprogramming the genome for development and therapy, Theriogenology, 2003, vol. 59, pp. 21–32.

    Article  PubMed  CAS  Google Scholar 

  3. Cheng, H.C., Zhang, F.W., Deng, C.Y., et al., NNAT and DIRAS3 genes are paternally expressed in pigs, Genet. Sel. Evol., 2007, vol. 39, pp. 599–607.

    Article  PubMed  CAS  Google Scholar 

  4. Zhang, F.W., Cheng, H.C., Jiang, C.D., et al., Imprinted status of pleomorphic adenoma gene-like I and paternal expression gene 10 genes in pigs, J. Anim. Sci., 2007, vol. 85, pp. 886–890.

    Article  PubMed  CAS  Google Scholar 

  5. Cheng, H.C., Zhang, F.W., Jiang, C.D., et al., Isolation and imprinting analysis of the porcine DLX5 gene and its association with carcass traits, Anim. Genet., 2008, vol. 39, pp. 395–399.

    Article  PubMed  CAS  Google Scholar 

  6. Morison, I.M., Ramsay, J.P., Spencer, H.G., A Census of mammalian imprinting. Trends in Genetics, 2005, vol. 21, pp. 457–465.

    Article  PubMed  CAS  Google Scholar 

  7. Cheng, H.C., Zhang, F.W., Deng, C.Y., et al., ASCL2 gene expression analysis and its association with carcass traits in pigs, Asian-Aust. J. Anim. Sci., 2007, vol. 20, pp. 1485–1489.

    CAS  Google Scholar 

  8. Nakanishi, H., Obaishi, H., Satoh, A., et al., Neurabin: a novel neural tissue-specific actin filament-binding protein involved in neurite gormation. J. Cell Biol., 1997, vol. 139, pp. 951–961.

    Article  PubMed  CAS  Google Scholar 

  9. Ono, R., Shiura, H., Aburatani, A., et al., Identification of a large novel imprinted gene cluster on mouse proximal chromosome 6, Genome Res., 2003, vol. 13, pp. 1696–1705.

    Article  PubMed  CAS  Google Scholar 

  10. Nakabayashi, K., Makino, S., Minagawa, S., et al., Genomic imprinting of PPP1R9A encoding neurabin I in skeletal muscle and extra-embryonic tissues, J. Med. Genetics, 2004, vol. 41, pp. 601–608.

    Article  CAS  Google Scholar 

  11. Loyola, A., and Almouzni, G., Histone chaperones, a supporting role in the limelight, Biochim. Biophys. Acta, 2004, vol. 1677, pp. 3–11.

    PubMed  CAS  Google Scholar 

  12. Wood, A.J., Roberts, R.G., Monk, D., et al., A Screen for retrotransposed imprinted genes reveals an association between X chromosome homology and maternal germ-line methylation, PLoS Genetics, 2007, vol. 3, pp. 192–203.

    Article  CAS  Google Scholar 

  13. Smith, R.J., Dean, W., Konfortova, G., and Kelsey, G., Identification of novel imprinted genes in a genomewide screen for maternal methylation, Genome Res., 2003, vol. 13, pp. 558–569.

    Article  PubMed  CAS  Google Scholar 

  14. Zaitoun, I., and Khatib, H., Assessment of genomic imprinting of SLC38A4, NNAT, NAP1L5, and H19 in cattle, BMC Genetics, 2003, vol. 7, pp. 49–58.

    Article  Google Scholar 

  15. Relaix, F., Wei, X.J., Wu, X., and Sassoon, D.A., Peg3/Pw1 is an imprinted gene involved in the TNF-NFkB signal transduction pathway, Nat. Genet., 1998, vol. 18, pp. 287–291.

    Article  PubMed  CAS  Google Scholar 

  16. Relaix, F., Wei, X., Li, W., et al., Pw1/Peg3 is a potential cell death mediator and cooperates with siah1a in p53-mediated apoptosis, Proc. Natl Acad. Sci. USA, 2000, vol. 97, pp. 2105–2110.

    Article  PubMed  CAS  Google Scholar 

  17. Kuroiwa, Y., Kaneko-Ishino, T., Kagitani, F., et al., Peg3 imprinted gene on proximal chromosome 7 encodes for a zinc finger protein, Nature Genetics, 1997, vol. 121, pp. 186–190.

    Google Scholar 

  18. Murphy, S.K., Wylie, A.A., and Jirtle, R.L., Imprinting of PEG3, the human homologue of a mouse gene, Genomics, 2001, vol. 71, pp. 110–117.

    Article  PubMed  CAS  Google Scholar 

  19. Kim, J., Bergmann, A., Choo, J.H., and Stubbs, L., Genomic organization and imprinting of the Peg3 domain in bovine, Genomics, 2007, vol. 90, pp. 85–92.

    Article  PubMed  Google Scholar 

  20. Thurston, A., Taylor, J., Gardner, J., et al., Monoallelic expression of nine imprinted genes in the sheep embryo occurs after the blastocyst stage, Reproduction, 2008, vol. 135, pp. 29–40.

    Article  PubMed  CAS  Google Scholar 

  21. Burnett, P.E., Blackshaw, S., Lai, M.M., et al., Neurabin is a synaptic protein linking p70 S6 kinase and the neuronal cytoskeleton, Proc. Natl Acad. Sci. USA, 1998, vol. 95, pp. 8351–8356.

    Article  PubMed  CAS  Google Scholar 

  22. Duhadaway, J., Rowe, F., Elliott, K., et al., Bau, a splice form of neurabin-i that interacts with the tumor suppressor bin1, inhibits malignant cell transformation, Cell Adhes. Commun., 1999, vol. 7, pp. 99–110.

    Article  PubMed  CAS  Google Scholar 

  23. Bischoff, S.R., Tsai, S., Hardison, N., et al., Characterization of conserved and nonconserved imprinted genes in swine, Biol. Reproduction, 2009, vol. 81, pp. 906–920.

    Article  CAS  Google Scholar 

  24. Davies, W., Smith, R.J., Kelsey, G., and Wilkinson, L.S., Expression patterns of the novel imprinted genes Nap1l5 and Peg13 and their non-imprinted host genes in the adult mouse brain, Gene Expression Patterns, 2004, vol. 4, pp. 741–747.

    Article  PubMed  CAS  Google Scholar 

  25. Kim, J., Bergmann, A., and Stubbs, L., Exon sharing of a novel human zinc-finger gene, ZIM2, and paternally expressed gene 3 (PEG3), Genomics, 2000, vol. 64, pp. 114–118.

    Article  PubMed  CAS  Google Scholar 

  26. Kim, J., Ashworth, L., Branscomb, E., and Stubbs, L., The human homolog of a mouse-imprinted gene, Peg3, maps to a zinc finger generich region of human chromosome 19q13.4, Genome Res., 1997, vol. 7, pp. 532–540.

    PubMed  CAS  Google Scholar 

  27. Kim, J., Bergmann, A., Lucas, S., et al., Lineage-specific imprinting and evolution of the zinc-finger gene ZIM2, Genomics, 2004, vol. 84, pp. 47–58.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Life Science, Southwest University, Chongqing, 400715, China

    C. D. Jiang & S. Li

  2. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Ministry of Education, Huazhong Agricultural University. Wuhan, China University, Chongqing, 400715, China

    C. Y. Deng

Authors
  1. C. D. Jiang
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  2. S. Li
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  3. C. Y. Deng
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Correspondence to C. D. Jiang.

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Jiang, C.D., Li, S. & Deng, C.Y. Assessment of genomic imprinting of PPP1R9A, NAP1L5 and PEG3 in pigs. Russ J Genet 47, 471–476 (2011). https://doi.org/10.1134/S1022795411040053

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  • DOI: https://doi.org/10.1134/S1022795411040053

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