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Physiological and Biochemical Responses of Lettuce to Thymol, as Allelochemical

Abstract

Nowadays, allelopathic investigations have focused to identify action mechanisms of this compounds on target organisms in natural habitats. It may tend to introduce new natural herbicides and pesticides for replacing the synthetic ones. In the present study, the allelopathic effects of thymol on lettuce (Lactuca sativa L.) as a model plant were investigated in physiological and biochemical aspects. At the first stage, the effect of different concentrations of thymol on some growth parameters, including seed germination, radicle and plumule growth were evaluated to determine the optimum concentration for the continuation of our experiment. Then, the lettuce seedlings were cultured in pots containing peat and were irrigated with Hoagland nutrient solution supplemented with thymol at the concentration of 50 and 100 μg/mL. The effect of these treatments on physiological, biochemical aspects of the plant were studied following the plant growth. The results showed whereas, the shoot fresh and dry weights and photosynthetic rate in the treated group were decreased significantly compared to the control group at P ≤ 0.05, photochemical efficiency of photosystem II, total protein concentration, proline content and the activity of some antioxidant enzymes such as polyphenol oxidase, ascorbate peroxidase and catalase were increased in treated plants than control ones. However, there was no significantly change in chlorophyll content in treated group than control. It can be concluded that the thymol as an allelochemical caused some physiological and biochemical responses in the lettuce which are much similar to induced responses under abiotic stress. Indeed, it induces a kind of stress named as allelochemical stress.

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References

  1. Reigosa, M.J., Pedrol, N., and Gonzalez, L., Allelopathy: A Physiological Process with Ecological Implications, Dordrecht: Springer, 2006.

    Book  Google Scholar 

  2. Narwal, S.S. and Tauro, P., Suggested methodology for allelopathy laboratory bioassay, in Allelopathy: Field Observation and Methodology, Narwal, S.S., Ed., Joudpur: Sci. Publ., 1996, pp. 255–265.

    Google Scholar 

  3. Naylov, R.E.L., Weed Management Handbook, Oxford: Blackwell, 2002.

    Google Scholar 

  4. Numpaque, M.A., Oviedo, L.A., Gil, J.H., García, C.M., and Durango, D.L., Thymol and carvacrol: biotransformation and antifungal activity against the plant pathogenic fungi Colletotrichum acutatum and Botryodiplodia theobromae, Trop. Plant Pathol., 2011, vol. 36, pp. 3–13.

    Article  Google Scholar 

  5. Pérez-Vásquez, A., Linares, E., and Bye, R., Cerda-García-Rojas, C.M., and Mata, R., Phytotoxic activity and conformational analysis of thymol analogs from Hofmeisteria schaffneri, Phytochemistry, 2008, vol. 69, pp. 1339–1347.

    Article  PubMed  CAS  Google Scholar 

  6. Razavi, S.M., Heseinzadeh, S., and Navid, S.L., The effects of (–)-carvone as an allelochemical compound on germination, growth and activity of enzymes in lettuce plants, Plant Stress Physiol., 2014, vol. 1, pp. 35–42.

    Google Scholar 

  7. Bradford, M.M., A rapid and sensitive method for the quantitation of protein utilizing the principle of protein–dye binding, J. Anal. Biochem., 1976, vol. 72, pp. 248–254.

    Article  CAS  Google Scholar 

  8. Mostefaii, A., Protein Gel Electrophoresis, A Guide to Theory and Practice, Tehran: Tazkiyeh Publ., 1999.

    Google Scholar 

  9. Bogatek, R. and Gniazdowska, A., ROS and phytohormones in plant–plant allelopathic interaction, Plant Signal. Behav., 2007, vol. 2, pp. 317–318.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Shen, Q., Zhou, W., Li, H., Hu, L., and Mo, H., ROS involve the fungicidal actions of thymol against spores of Aspergilus flavus via the induction of nitric oxide, PLoS One, 2016, vol. 11, pp. 1–14.

    Google Scholar 

  11. Razavi, S.M. and Nejad-Ebrahimi, S., Chemical composition, allelopathic and cytotoxic effects of essential oils of flowering tops and leaves of Crambe orientalis, Nat. Prod. Res., 2009, vol. 23, pp. 1492–1498.

    Article  PubMed  CAS  Google Scholar 

  12. Razavi, S.M. and Nejad-Ebrahimi, S., Phytochemical analysis and allelopathic activity of essential oils of Ecballium elaterium A. Richard growing in Iran, Nat. Prod. Res., 2010, vol. 24, pp. 1704–1709.

    Article  PubMed  CAS  Google Scholar 

  13. Li, B., Cheng, B., Zhang, J., Wang, P., and Feng, B., The environmental fate of thymol, a novel botanical pesticide, in tropical agricultural soil and water, Toxicol. Environ. Chem., 2017, vol. 99, pp. 223–232.

    Article  CAS  Google Scholar 

  14. Miller, D.A., Allelopathy in forage crop systems, Agron. J., 1996, vol. 88, pp. 854–859.

    Article  Google Scholar 

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Correspondence to S. M. Razavi.

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Nasrollahi, P., Razavi, S.M., Ghasemian, A. et al. Physiological and Biochemical Responses of Lettuce to Thymol, as Allelochemical. Russ J Plant Physiol 65, 598–603 (2018). https://doi.org/10.1134/S1021443718040167

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  • DOI: https://doi.org/10.1134/S1021443718040167

Keywords

  • Lactuca sativa
  • thymol
  • allelopathy
  • ascorbate peroxidase
  • polyphenol oxidase
  • catalase