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The inhibition of mitochondrial metabolic activity in etiolated pea seedlings under water stress

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Abstract

In the present work, we studied the influence of water (osmotic) stress on mitochondrial metabolic activity in etiolated pea (Pisum sativum L.) seedlings. Three-day-old pea seedlings were subjected to stress by placing their roots in 0.6 M mannitol for 48 h. Epicotyl growth was severely suppressed, and tissue water content was decreased. We revealed the negative influence of the water stress on mitochondrial metabolic activity of seedlings, which effect was retained also after organelle isolation. In particular, in the mitochondria of stressed seedlings, the rate of oxidation of malate and other respiratory substrates (in state 3) was severely decreased, as well as respiratory control ratio. The rate of proline oxidation was reduced most seriously (by 70%). The efficiency of oxidative phosphorylation, according to the ADP/O ratio was not changed or was increased as compared to mitochondria in control plants. Activation of CN-resistant oxidase and other alternative pathways of electron transport in the mitochondrial electron-transport chain in stressed plants were not observed. In the epicotyl tissues under water stress, no MDA was accumulated and proline accumulation was insignificant. The role of mitochondria in adaptation responses of young seedlings is discussed.

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Abbreviations

AO:

mitochondrial alternative oxidase

FA:

fatty acid(s)

POL:

peroxidation of lipids

RCR:

respiratory control ratio

SHAM:

salicylhydroxamic acid

TCA:

tricarboxylic acid cycle

TBA:

thiobarbituric acid

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127726, Russia

    I. P. Generozova, S. N. Maevskaya & A. G. Shugaev

Authors
  1. I. P. Generozova
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  2. S. N. Maevskaya
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  3. A. G. Shugaev
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Correspondence to A. G. Shugaev.

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Original Russian Text © I.P. Generosova, S.N. Maevskaya, A.G. Shugaev, 2009, published in Fiziologiya Rastenii, 2009, Vol. 56, No. 1, pp. 45–52.

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Generozova, I.P., Maevskaya, S.N. & Shugaev, A.G. The inhibition of mitochondrial metabolic activity in etiolated pea seedlings under water stress. Russ J Plant Physiol 56, 38–44 (2009). https://doi.org/10.1134/S1021443709010063

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