Herald of the Russian Academy of Sciences

, Volume 87, Issue 2, pp 143–149 | Cite as

Molecular and biotechnological methods of creating genetic resources for vegetable crops

  • B. F. Pivovarov
  • O. N. Pyshnaya
  • L. K. Gurkina
  • T. S. Naumenko
Scientific Session of the RAS General Meeting
  • 29 Downloads

Abstract

The results obtained by the research team of the All-Russia Research Institute of Selection and Seed Breeding for Vegetable Crops are presented. Specific examples demonstrate the efficacy of methods and technologies that create genetic resources for vegetable crops: the cytogenetic GISH and FISH methods, the doubled haploid technology in the culture of unpollinated ovules, and molecular marking used as a specific and varietal identifier of vegetable crops and for the study and identification of genes responsible for agronomic characters. The results of regular phytosanitary monitoring are considered separately: identifying the composition of pathogenic species not recorded previously in vegetable crops in individual regions and throughout the country in general and developing the scientific basis for the evaluation and selection of vegetable crops with a highly effective antioxidant system to design socially and economically important functional food products.

Keywords

genetic resources vegetable farming selection and seed breeding varieties and hybrids sources and donors of economically valuable characters biotechnological and molecular-genetic methods interspecific hybridization phytopathological monitoring antioxidants functional food products 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    N. I. Timin, O. N. Pyshnaya, A. F. Agafonov, et al., Interspecific Hybridization of Vegetable Plants (Allium L., Onion; Daucus L., Carrot; Capsicum L., Pepper) (Izd. VNIISSOK, Moscow, 2013) [in Russian].Google Scholar
  2. 2.
    N. I. Timin and L. T. Timina, “Selection-genetic identification of inbred forms and lines of carrot,” Selek. Semenovod. Ovoshch. Kul’tur, No. 46, 555–560 (2015).Google Scholar
  3. 3.
    E. P. Pronina, I. P. Kotlyar, V. A. Ushakov, and A. A. Antoshkin, “Selection of vegetable legume crops at the FGBNU VNIISSOK from its foundation,” in Grain Legume Crops As a Trend Developing in Russia. The First International Forum (Omsk. Gos. Agric. Univ. Im. P.A. Stolypina, Omsk, 2016), pp. 18–24.Google Scholar
  4. 4.
    The Eggplant Solanum spp., Ed. by V. F. Pivovarov (VNIISSOK, Moscow, 2015).Google Scholar
  5. 5.
    V. M. Verba, M. I. Mamedov, O. N. Pyshnaya, and N. A. Shmykova, “Phenolic compounds in the fruits of various eggplant species (Solanum melongena, S. integrifolium, S. aethiopicum) and their VNIISSOK-bred hybrids F1,” Ovoshchi Rossii, No. 3, 28–33 (2011).Google Scholar
  6. 6.
    I. A. Engalycheva, O. N. Pyshnaya, E. A. Dzhos, et al., “Interspecific hybridization in breeding pepper and lettuce for resistance to viral infections,” Russ. Agric. Sci. Rev. 6, 2–4 (2015).Google Scholar
  7. 7.
    M. V. Budylin, L. I. Khrustaleva, L. Y. Kan, and V. S. Romanov, “GISH study of advanced generation of the interspecific hybrids between Allium Cepa L. and Allium Fistulosum L. with relative resistance to downy mildew,” Russ. J. Genet. 50, 387–394 (2014).CrossRefGoogle Scholar
  8. 8.
    N. A. Shmykova, O. N. Pyshnaya, D. V. Shumilina, and E. A. Dzhos, “Morphological characteristics of pepper doubled haploid plants obtained through anther/microspore in vitro culture of the interspecific hybrids of Capsicum annuum L. and C. chinense Jacq,” Dokl. RASKhN, No. 5, 21–25 (2014).Google Scholar
  9. 9.
    N. A. Shmykova, D. V. Shumilina, and T. P. Suprunova, “Obtaining doubled haploids in species of the genus Brassica L.,” Vavilov. Zh. Genet. Selek. 19, 111–120 (2015).Google Scholar
  10. 10.
    D. V. Shumilina, N. A. Shmykova, L. L. Bondareva, and T. P. Suprunova, “Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp. chinensis) cv. Lastochka,” Biol. Bull. 42, 302–309 (2015).CrossRefGoogle Scholar
  11. 11.
    A. I. Batmanova, L. L. Bondareva, D. V. Shumilina, et al., “Assessing hybrid combinations of white cabbage using a doubled haploid line for the complex of economically valuable characters,” Selek. Semenovodstvo Ovoshch. Kul’tur, No. 46, 118–125 (2015).Google Scholar
  12. 12.
    N. A. Shmykova, D. V. Shumilina, L. L. Bondareva, and E. A. Zablotskaya, “Improving broccoli DH technologies,” Selek. Semenovodstvo Ovoshch. Kul’tur, No. 46, 601–608 (2015).Google Scholar
  13. 13.
    N. A. Shmykova and T. P. Suprunova, “Gynogenesis induction in the in vitro culture of unpollinated ovules of Cucumis sativus L.,” Gavrish, No. 4, 40–44 (2009).Google Scholar
  14. 14.
    E. A. Domblides, N. A. Shmykova, T. V. Zayachkovskaya, et al., “Doubled haploid plant production from in vitro culture of unpollinated ovules of summer squash (Cucurbita pepo L.),” in Biotechnology As a Tool of Preserving the Biodiversity of the Plant Kingdom (Physiological, Biochemical, Embryological, Genetic, and Legal Aspects). Proceedings of the VII International Scientific and Practical Conference Dedicated to the 30th Anniversary of the Department of Plant Biotechnology of the Nikitskii Botanical Garden (IT ARIAL, Simferopol’, 2016), pp. 28–29 [in Russian].Google Scholar
  15. 15.
    A. S. Domblides, E. A. Domblides, V. S. Romanov, et al., “Polymorphism of ISSR and SSR markers in representatives of the genus Allium L.,” in Biotechnology As a Tool of Preserving the Biodiversity of the Plant Kingdom (Physiological, Biochemical, Embryological, Genetic, and Legal Aspects). Proceedings of the VI International Scientific Practical Conference (IT ARIAL, Simferopol’, 2014), p. 163.Google Scholar
  16. 16.
    E. A. Domblides, A. S. Domblides, T. V. Zayachkovskaya, and L. L. Bondareva, “Identification of cytoplasm types in accessions of the brassicaceae family (Brassicaceae Burnett) by DNA markers,” Russ. J. Genet.: Applied Res. 6, 510–519 (2016).CrossRefGoogle Scholar
  17. 17.
    O. N. Pyshnaya, M. I. Mamedov, N. A. Shmykova, et al., “Classical and modern methods in pepper Capsicum L. selection,” Proc. Kuban. Gos. Agric. Univ., No. 55, 213–216 (2015).Google Scholar
  18. 18.
    T. P. Suprunova, A. N. Logunov, V. V. Logunova, and A. F. Agafonov, “Determining the type of cytoplasmic male sterility of VNIISSOK-bred bulb onion (Allium cepa L.) using molecular markers,” Ovoshchi Rossii, No. 4, 20–21 (2011).Google Scholar
  19. 19.
    E. A. Snigir’, O. N. Pyshnaya, E. Z. Kochieva, and N. N. Ryzhova, “AFLP analysis of varietal polymorphism of Capsicum annuum L.,” Sel’skokhoz. Biol., No. 1, 53–60 (2013).Google Scholar
  20. 20.
    L. T. Timina and I. A. Engalycheva, “Complex of pathogens in vegetable crops in the Central Region of Russia,” Ovoshchi Rossii, Nos. 3, 4, 123–129 (2015).Google Scholar
  21. 21.
    I. A. Engalycheva and O. V. Pavlova, “Interspecific hybridization of lettuce (Lactuca sativa L.) in breeding for resistance to Tomato aspermy cucumovirus,” Vestn. Zashchity Rastenii 89, 68–70 (2016).Google Scholar
  22. 22.
    I. A. Engalycheva, T. I. Pleshakova, A. V. Gapeka, and L. T. Timina, “Monitoring especially hazardous viral diseases of legume family crops in the conditions of Moscow oblast,” in Improving the Efficacy of Agricultural Science in Modern Conditions. Proc. of the International Scientific and Practical Conference of Young Scientists and Specialists, November 17—18, 2015 (FGBNU VNII of Grain Legume and Cereal Crops, Orel, 2015), pp. 41–44.Google Scholar
  23. 23.
    A. F. Agafonov, L. T. Timina, and K. S. Shestakova, “Onion growers, watch out for black mold rot of onions,” Ovoshchi Rossii, No. 3 (2012).Google Scholar
  24. 24.
    V. F. Pivovarov, V. P. Nikul’shin, L. T. Timina, and K. S. Shestakova, “Pathogenic microflora of winter garlic,” Vestn. RASKhN, No. 5, 63–64 (2009).Google Scholar
  25. 25.
    I. Yu. Kondrat’eva, M. S. Gins, V. K. Gins, and M. S. Il’enko, “Hereditary features of early ripening and lycopene contents in tomato hybrids F1,” Vestn. RASKhN, No. 5, 46–48 (2011).Google Scholar
  26. 26.
    K. H. Torres Mino, Assessing amaranth varieties with biochemical and molecular methods to create functional products based on leaf biomass. Extended Abstract of Cand. Sci. (Agric.) Dissertation, Moscow, 2015.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • B. F. Pivovarov
    • 1
  • O. N. Pyshnaya
    • 1
  • L. K. Gurkina
    • 1
  • T. S. Naumenko
    • 1
  1. 1.All-Russia Research Institute of Selection and Seed Breeding for Vegetable CropsVNIISSOK SettlementMoscowRussia

Personalised recommendations