Activated T cells from peripheral blood of six volunteers, participants of the experiment with 21-day dry immersion (DI), were analyzed. The results of analysis of the synthesis of cytokines affecting the metabolism of bone tissue and contributing to the maintenance of its physiological homeostasis are presented. We have shown that prolonged restriction of motor loads leads to an increase in the content of peripheral blood activated CD3+CD25+ T cells. The monocultures of peripheral blood CD3+ cells stimulated in vitro with anti-CD2/CD3/CD28 complex for 24 h revealed changes in the cytokines synthesis. Two different types of response of activated T cells to prolonged exposure to DI have been demonstrated: either an increase or a decrease in the secretion of cytokines stimulating increased bone resorption (IL-1β, IL-6, IL-8, IL-17A, TNF-α, GM-CSF) and weakening osteoclastogenesis (IFN-γ, IL-3, IL-4, IL-10, IL-13).
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This study was supported by the Program of Scientific Research of the Presidium of the Russian Academy of Sciences “Basic Research for Biomedical Technologies” for 2018–2020: “Fundamental Research for Technologies for Disease Prevention, Human Rehabilitation and Targeted Increase of Resistance to Adverse Factors” and basic topic 65.1 in the framework of the State Contract with the Institute of Biomedical Problems of the Russian Academy of Sciences.
COMPLIANCE WITH ETHICAL STANDARDS
All the subjects received admission from the medical expert commission and signed informed consent to participate in the experiment in accordance with the Helsinki Declaration. The research program was approved at a meeting of the Academic Council (Protocol no. 6 of June 27, 2018) and approved by the Biomedical Ethics Commission of the Institute of Biomedical Problems of the Russian Academy of Sciences (Protocol no. 483 of August 3, 2018).
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
Translated by I. Shipounova
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Kutko, O.V., Rykova, M.P., Antropova, E.N. et al. Effect of 21-day Dry Immersion on the Production of Cytokines Involved in the Regulation of Bone Metabolism by T Cells. Hum Physiol 46, 787–791 (2020). https://doi.org/10.1134/S0362119720070099
- dry immersion
- immune system
- T cells