Russian Journal of Physical Chemistry A

, Volume 92, Issue 5, pp 922–926 | Cite as

Algorithm for Calculating the Dissociation Constants of Ampholytes in Nonbuffer Systems

  • S. S. Lysova
  • T. A. Skripnikova
  • Yu. E. Zevatskii
Physical Chemistry of Solutions


An algorithm for calculating the dissociation constants of ampholytes in aqueous solutions is developed on the basis of spectrophotometric data in the UV and visible ranges without pH measurements of a medium and without buffer solutions. The proposed algorithm has been experimentally tested for five ampholytes of different strengths. The relative error of measuring dissociation constants is less than 5%.


thermodynamic dissociation constant spectrophotometric method UV–visible range aqueous solutions 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Wilson and Gisvold’s Textbook of Organic Medicinal and Pharmaceutical Chemistry, 12th ed., Ed. by J. M. Beale, Jr. and J. H. Block (Lippincott Williams Wilkins, Philadelphia, PA, 2011).Google Scholar
  2. 2.
    J. E. A. Comer and D. Manallack, Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (Elsevier, Amsterdam, 2014), p.41.Google Scholar
  3. 3.
    L. Di and E. H. Kerns, Drug-Like Properties (Elsevier, Amsterdam, 2016), p.307.CrossRefGoogle Scholar
  4. 4.
    A. Pagliara, P. Carrupt, G. Caron, P. Gaillard, and B. Testa, Chem. Rev. 97, 3385 (1997).CrossRefGoogle Scholar
  5. 5.
    A. Avdeef, Curr. Top. Med. Chem. 1, 277 (2001).CrossRefGoogle Scholar
  6. 6.
    K. Takacs-Novak and K. Y. Tam, J. Pharm. Biomed. Anal. 21, 1171 (2000).CrossRefGoogle Scholar
  7. 7.
    S. S. Lysova, T. A. Skripnikova, and Yu. E. Zevatskii, Russ. J. Phys. Chem. A 91, 2366 (2017).CrossRefGoogle Scholar
  8. 8.
    I. M. Korenman, Analytical Chemistry of Small Concentrations (Khimiya, Moscow, 1966), p.111.Google Scholar
  9. 9.
    E. M. Goncalvesm, T. S. Rego, M. E. Minas de Piedade, J. Chem. Thermodyn., No. 43, 974 (2011).CrossRefGoogle Scholar
  10. 10.
    J. A. Dean, Lange’s Handbook of Chemistry (McGraw-Hill, New York, 1999).Google Scholar
  11. 11.
    A. Kristl, A. Mrhar, and F. Kozjek, Int. J. Pharm. 99, 79 (1993).CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • S. S. Lysova
    • 1
  • T. A. Skripnikova
    • 1
  • Yu. E. Zevatskii
    • 1
    • 2
    • 3
  1. 1.St. Petersburg State University of Industrial Technologies and DesignSt. PetersburgRussia
  2. 2.ZAO NovbytkhimGatchinaRussia
  3. 3.St. Petersburg State Electrotechnical University (LETI)St. PetersburgRussia

Personalised recommendations