, Volume 87, Issue 2, pp 282–289 | Cite as

Isolation and Characterization of Bacterial Species from Ain Mud Volcano, Iran

  • E. Sepanian
  • A. A. Sepahy
  • F. Hosseini
Experimental Articles


Along the coastal belt of Makran, on the southeastern margin of Iran, there are several active and inactive mud volcanoes with different morphologies. Ain—an actively bubbling eye-shaped mud pool—is one of these unique geological phenomena. Present study indicates the first overview of the bacterial diversity of this mud volcano obtained by culture techniques. For this purpose, two samples were collected at two different depths of the mud pool and were cultivated on two different nutrient culture media. A total of 13 isolates were randomly chosen for further phenotypic and genotypic characterization. Growth of the isolates occurred at 25–42°C, pH 8–10 and 0–10% NaCl, indicating that most of them were haloalkaliphiles. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the bacterial isolates belonged to three major taxa: Gammaproteobacteria (genera: Marinobacter, Nitrincola, Stentrophomonas), Actinobactera (genera: Kocuria, Brevibacterium, Dietzia) and Firmicutes (genus Planomicrobium). Gammaproteobacteria were the most abundant, following Actinobacteria and Firmicutes. Most of the strains isolated in the present study had the ability to produce extracellular hydrolytic enzymes such as lipases, amylases, proteases and DNases, making them important biotechnologically.


bacterial diversity Ain Mud Volcano haloalkaliphile culture techniques 


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© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Microbiology, Faculty of Biological ScienceIslamic Azad University, North-Tehran BranchTehranIran

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