Hybrid Selection of Saccharomyces cerevisiae Yeasts for Thermotolerance and Fermentation Activity
- 32 Downloads
Molecular genetic screening of Saccharomyces yeasts, isolated from natural sources in the regions of the world with a hot climate (Africa, South America, Southeast and Central Asia) was used for the search of thermotolerant S. cerevisiae strains. Based on physiological tests, four strains were selected that could grow at high temperatures (42 and 43°C) and had good fermentation activity: 7962-4B, 3529-7B, 52922-4-1-1A- 1C, and 87-2421.1-2A. Hybrids of monosporic culture of distiller’s race XII (XII7-2) with the thermotolerant strains were obtained. Unlike the strain XII7-2, which is unable to grow at above 39°C, all hybrids showed good growth at 42°C. Two of the six hybrids analyzed, H2-1 (87-2421.1-2A × XII7-2) and H3-2 (7962-4B × XII7-2), showed higher fermentation activity than the parental strains. According to the results obtained, inter-strain hybridization is an efficient method of obtaining S. cerevisiae strains, which combine thermotolerance with high efficiency of alcoholic fermentation.
KeywordsSaccharomyces cerevisiae distiller yeasts inter-strain hybridization heterosis
Unable to display preview. Download preview PDF.
- Cai, J., Zhang, B., and Liu, Y., Hybridization and selection of yeasts. IV. Breeding of yeasts for high ethanol production, Acta Microbiol. Sin., 1982, vol. 22, pp. 48–54.Google Scholar
- Inge-Vechtomov, S.G., New genetic lines of Saccharomyces cerevisiae yeasts, Vestn. LGU, 1963, no. 21, pp. 117–125.Google Scholar
- Kiritsis, S., New, more environmentally friendly and productive sources for satisfying the rapidly growing requirements of transportation for bioethanol, Energ. Vestn., 2011, vol. 10, no. 1, pp. 9–29.Google Scholar
- McMillan, J.D., Newman, M.M., Templeton, D.W., and Mohagheghi, A., Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis, Appl. Biochem. Biotechnol., 1999, vols. 77–79, pp. 649–666.CrossRefPubMedGoogle Scholar
- Naseeb, S., James, S.A., Alsammar, H., Michaels, C.J., Gini, B., Nueno-Palop, C., Bond, C.J., McGhie, H., Roberts, I.N., and Delneri, D., Saccharomyces jurei sp. nov., isolation and genetic identification of a novel yeast species from Quercus robur Naseeb et al., Int. J. Syst. Evol. Microbiol., 2017, vol. 67, pp. 2046–2052.CrossRefPubMedPubMedCentralGoogle Scholar
- Naumov, G.I., James, S.A., Naumova, E.S., Louis, E.J., and Roberts, I.N., Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharo-myces kudriavzevii and Saccharomyces mikatae, Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 1931–1942.CrossRefGoogle Scholar
- Serpova, E.V., Kishkovskaya, S.A., Martynenko, N.N., and Naumova, E.S., Molecular genetic identification of Crimean wine yeasts, Biotekhnologiya, 2011, no. 6, pp. 47–54.Google Scholar
- Vaughan-Martini, A. and Martini, A., Saccharomyces Meyen ex Reess (1870), in The Yeast, a Taxonomic Study, 5th ed., Kurtzman, C.P., Fell, J.W., and Boekhout, T., Eds., Amsterdam: Elsevier}}, 2011, vol. 2, pp. 733–746.Google Scholar