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Comparative Analysis of the Biological Activity and Chromatographic Profiles of the Extracts of Beauveria bassiana and B. pseudobassiana Cultures Grown on Different Nutrient Substrates

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Abstract

Soil, enodphytic, and insect-pathogenic micromycetes of the genus Beauveria are widespread in nature and are important producers on mycoinsecticides, enzymes, and pharmacologically usable and toxic compounds. The goal of the work was to determine chemodiagnostic approaches to differentiation of Beauveria cryptic species using the strains B. bassiana BBL and B. pseudobassiana BCu22 by comparing their toxicological properties (insecticidal, antimicrobial, phytotoxic, cytotoxic, and esterase-inhibition activity) and metabolite profiles (TLC and HPLC/DAD patterns) of the extracts from the cultures of these fungi growing on different loose substrates, on solid and liquid media. It was shown that when the strains were cultured in liquid media (SDAY and Adámek medium) and on solid substrates (millet and Czapek agar medium), they could be differentiated by the extract yield and chromatographic profiles, as well as by their insecticidal, antifungal, and cytotoxic activity. Thus, antifungal properties were more pronounced in B. pseudobassiana BCu22 grown in liquid Adámek and SDAY media, while cytotoxic properties were more notable in B. bassiana BBL grown in Adámek medium and on millet. Insecticidal properties of the extracts from these cultures varied depending on the substrate composition. Since the extracts of the studied fungi exhibited a broad spectrum of biological activity, the toxic properties of Beauveria spp. should be considered in the course of assessment of safety of these fungi as bioinsecticides.

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References

  • Anke H., Insecticidal and nematicidal metabolites from fungi, in The Mycota, vol. 10, Industrial Applications, Hofrichter, M., Ed., Berlin: Springer, 2010, pp. 151–163.

    Google Scholar 

  • Begley, C.G. and Waggoner, P., Soft contact lens contamination by Beauveria bassiana, Int. Contact Lens Clinic., 1992, vol. 19, pp. 247–251.

    Article  Google Scholar 

  • Berestetskii, A.O., Yuzikhin, O.S., Katkova, A.S., Dobrodumov, A.V., Sivogrivov, D.E., and Kolombet, L.V., Isolation, identification, and characteristics of the phytotoxin produced by the fungus Alternaria cirsinoxia, Appl. Biochem. Microbiol., 2010, vol. 46, pp. 75–79.

    Article  CAS  Google Scholar 

  • Bills, G.F., Platas, G., Fillola, A., Jiménez, M.R., Collado, J., Vicente, F., Martín, J., González, A., Bur-Zimmermann, J., Tormo, J.R., and Peláez, F., Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays, J. Appl. Microbiol., 2008, vol. 104, pp. 1644–1658.

    Article  CAS  PubMed  Google Scholar 

  • Bridge, P.D., Abraham, Y.J., Cornish, M.C., Prior, C., and Moore, D., The chemotaxonomy of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolates from the coffee berry borer Hypothenemus hampei (Coleoptera: Scolytidae), Mycopathologia, 1990, vol. 111, pp. 85–90.

    Article  CAS  Google Scholar 

  • Ellman, G.L., Courtney, K.D., Andres, V., Jr., and Feather-Stone, R.M., A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem. Pharmacol., 1961, vol. 7, pp. 88–95.

    CAS  Google Scholar 

  • Evidente, A., Berestetskiy, A., Cimmino, A., Tuzi, A., Superchi, S., Melck, D., and Andolfi, A., Papyracillic acid, a phytotoxic 1,6-dioxaspiro[4,4]nonene produced by Ascochyta agropyrina var. nana, a potential mycoherbicide for Elytrigia repens biocontrol, J. Agric. Food Chem., 2009, vol. 57, pp. 11168–11173.

    Article  CAS  PubMed  Google Scholar 

  • Fan, J., Xie, Y., Xue, J., and Liu, R., The effect of Beauveria brongniartii and its secondary metabolites on the detoxification enzymes of the pine caterpillar, Dendrolimus tabulaeformis, J. Insect Sci., 2013, vol. 13, article 44.

  • Frisvad, J.C. and Thrane, U., Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode-array detection), J. Chromatography, 1987, vol. 404, pp. 195–214.

    Article  CAS  Google Scholar 

  • Griffiths, D.A. and Jezequel, S.G., Metabolism of xenobiotics by Beauveria bassiana, Xenobiotica, 1993, vol. 23, pp. 1085–1100.

    Article  CAS  PubMed  Google Scholar 

  • Hamill, R.L., Higgens, C.E., Boaz, M.E., and Gorman, M., The structure of beauvericin, a new depsipeptide antibiotic toxic to Artemia salina, Tetrahedron Lett., 1969, vol. 10, pp. 4255–4258.

    Article  Google Scholar 

  • Hewage, R.T., Aree, T., Mahidol, C., Ruchirawat, S., and Kittakoop, P., One strain-many compounds (OSMAC) method for production of polyketides, azaphilones, and an isochromanone using the endophytic fungus Dothideomycete sp., Phytochemistry, 2014, vol. 108, pp. 87–94.

    Article  CAS  PubMed  Google Scholar 

  • Hu, Q., Li, F., and Zhang, Y., Risks of mycotoxins from mycoinsecticides to humans, BioMed Res. Int., 2016. Article ID 3194321, 13 pages. http://dx.doi.org/. doi 10.1155/2016/3194321

    Google Scholar 

  • Hyun, S.-H., Lee, S.-Y., Park, S.J., Kim, D.Y., Chun, Y.J., Sung, G.-H., Kim, S.H., and Choi, H.-K., Alteration of media composition and light conditions change morphology, metabolic profile, and beauvericin biosynthesis in Cordyceps bassiana mycelium, J. Microbiol. Biotechnol., 2013, vol. 23, pp. 47–55.

    Article  CAS  PubMed  Google Scholar 

  • Kadlec, Z., Šimek, P., Heydová, A., Jegorov, A., Maťha, V., Landa, Z., and Eyal, J., Chemotaxonomic discrimination among the fungal genera Tolypocladium, Beauveria and Paecilomyces, Biochem. Syst. Ecol., 1994, vol. 22, pp. 803–806.

    Article  CAS  Google Scholar 

  • Langenfeld, A., Blond, A., Gueye, S., Herson, P., Nay, B., Dupont, J., and Prado, S., Insecticidal cyclodepsipeptides from Beauveria feline, J. Nat. Prod., 2011, vol. 74, pp. 825–830.

    Article  CAS  PubMed  Google Scholar 

  • Lednev, G., Tokarev, Y., Uspanov, A., Malysh, J., Duisembekov, B., Sabitova, M., Levchenko, M., Smagulova, S., Orazova, S., Amanov, S., and Sagitov, A., Molecular criteria for screening of Beauveria strains used for insect pest control, J. Biotechnol., 2014, vol. 185, pp. S63–S64.

    Article  Google Scholar 

  • Molnár, I., Gibson, D.M., and Krasnoff, S.B., Secondary metabolites from entomopathogenic Hypocrealean fungi, Nat. Prod. Rep., 2010, vol. 27, pp. 1241–1275.

    Article  PubMed  Google Scholar 

  • Morais-Urano, R.P., Chagas, A.C.S., and Berlinck, R.G.S., Acaricidal action of destruxins produced by a marine-derived Beauveria felina on the bovine tick Rhipicephalus (Boophilus) microplus, Exper. Parasitol., 2012, vol. 132, pp. 362–366.

    Article  CAS  Google Scholar 

  • Mugnai, L., Bridge, P.D., and Evans, H.C., A chemotaxonomic evaluation of the genus Beauveria, Mycol. Res., 1989, vol. 92, pp. 199–209.

    Article  Google Scholar 

  • Neumann, K., Kehraus, S., Gütschow, M., and König, G.M., Cytotoxic and HLE-inhibitory tetramic acid derivatives from marine-derived fungi, Nat. Prod. Commun., 2009, vol. 4, pp. 347–354.

    CAS  PubMed  Google Scholar 

  • Quesada-Moraga, E. and Vey, A., Bassiacridin, a protein toxic for locusts secreted by the entomopathogenic fungus Beauveria bassiana, Mycol. Res., 2004, vol. 108, pp. 441–452.

    Article  CAS  PubMed  Google Scholar 

  • Reddy, G.V.P., Tangtrakulwanich, K., Wu, S., Miller, J.H., Ophus, V.L., Prewett, J., and Jaronski, S.T., Evaluation of the effectiveness of entomopathogens for the management of wireworms (Coleoptera: Elateridae) on spring wheat, J. Invertebrate Pathol., 2014, vol. 120, pp. 43–49.

    Article  Google Scholar 

  • Sarker, S.D., Latif, Z., and Gray, A.I., Natural product isolation. An overview, in Methods in Biotechnology, vol. 20, Natural Products Isolation, Sarker, S.D., Latif, Z., and Gray, A.I., Eds., Totowa: Humana Press, 2006, pp. 1–25.

    Google Scholar 

  • Strasser, H., Abendstein, D., Stuppner, H., and Butt, T.M., Monitoring the distribution of secondary metabolites produced by the entomogenous fungus Beauveria brongniartii with particular reference to oosporein, Mycol. Res., 2000, vol. 104, pp. 1227–1233.

    Article  CAS  Google Scholar 

  • Valencia, J.W., Gaitán Bustamante, A.L., Jiménez, A.V., and Grossi-de-Sá, M.F., Cytotoxic activity of fungal metabolites from the pathogenic fungus Beauveria bassiana: an intraspecific evaluation of beauvericin production, Curr. Microbiol., 2011, vol. 63, pp. 306–312.

    Article  CAS  PubMed  Google Scholar 

  • Vega, F.E., Goettel, M.S., Blackwell, M., Chandler, D., Jackson, M.A., Keller, S., Koike, M., Maniania, N.K., Monzón, A., Ownley, B.H., Pell, J.K., Rangel, D.E.N., and Roy, H.E., Fungal entomopathogens: new insights on their ecology, Fungal Ecol., 2009, vol. 2, pp. 149–159.

    Article  Google Scholar 

  • Wang, Q. and Xu, L., Beauvericin, a bioactive compound produced by fungi: a short review, Molecules, 2012, vol. 17, pp. 2367–2377.

    Article  CAS  PubMed  Google Scholar 

  • Westwood, G.S., Huang, S.-W., and Keyhani, N.O., Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana, Clin. Mol. Allergy, 2006, vol. 4. doi 10.1186/1476-7961-4-12

  • Yang, Z.P. and Dettbarn, W.D., Prevention of tolerance to the organophosphorus anticholinesterase paraoxon with carboxylesterase inhibitors, Biochem. Pharmacol., 1998, vol. 55, pp. 1419–1426.

    Article  CAS  PubMed  Google Scholar 

  • Yoon, C.-S., Yu, K.-W., Bae, S.-H., Song, H.-H., Park, H.-S., and Lee, C., Chemical properties and physiological activities of synnemata of Beauveria bassiana, J. Microbiol. Biotechnol., 2003, vol. 13, pp. 125–133.

    CAS  Google Scholar 

  • Yuan, W., Wang, P., Zhang, Z., and Li, S., Glycosylation of (–)-maackiain by Beauveria bassiana and Cunninghamella echinulata var. elegans, Biocat. Biotransform., 2010, vol. 28, pp. 117–121.

    Article  CAS  Google Scholar 

  • Zibaee, A., Bandani, A.R., and Tork, M., Effect of the entomopathogenic fungus, Beauveria bassiana, and its secondary metabolite on detoxifying enzyme activities and acetylcholinesterase (AChE) of the Sunn pest, Eurygaster integriceps (Heteroptera: Scutellaridae), Biocontrol Sci. Technol., 2009, vol. 19, pp. 485–498.

    Google Scholar 

  • Zimmermann, G., Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii, Biocontrol Sci. Technol., 2007, vol. 17, pp. 553–596.

    Article  Google Scholar 

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Authors and Affiliations

  1. All-Russian Research Institute of Plant Protection, St.-Petersburg, Russia

    A. O. Berestetskiy, A. N. Ivanova, M. O. Petrova, E. A. Stepanycheva & G. R. Lednev

  2. Research Institute for Hygiene, Occupational Pathology, and Human Ecology, St.-Petersburg, Russia

    D. S. Prokof’eva

  3. Kazakh Research Institute for Plant Protection and Quarantine, Almaty, Kazakhstan

    A. M. Uspanov

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  1. A. O. Berestetskiy
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  2. A. N. Ivanova
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  3. M. O. Petrova
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  4. D. S. Prokof’eva
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  5. E. A. Stepanycheva
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  6. A. M. Uspanov
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  7. G. R. Lednev
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Correspondence to A. O. Berestetskiy.

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Original Russian Text © A.O. Berestetskiy, A.N. Ivanova, M.O. Petrova, D.S. Prokof’eva, E.A. Stepanycheva, A.M. Uspanov, G.R. Lednev, 2018, published in Mikrobiologiya, 2018, Vol. 87, No. 2, pp. 146–161.

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Berestetskiy, A.O., Ivanova, A.N., Petrova, M.O. et al. Comparative Analysis of the Biological Activity and Chromatographic Profiles of the Extracts of Beauveria bassiana and B. pseudobassiana Cultures Grown on Different Nutrient Substrates. Microbiology 87, 200–214 (2018). https://doi.org/10.1134/S0026261718020030

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