A karyological analysis of the phytophilous chironomids Endochironomus tendens F. (Diptera, Chironomidae) from six water bodies of Saratov and Volgograd provinces has been carried out. The karyotype of E. tendens has 2n = 6, with morphologically well-defined centromeric zones represented by large heterochromatin blocks. In chromosome I (EF), arm E is characterized by five banding sequences: tendE1, tendE2, tendE3, tendE4, and tendE5; the band order for sequence tendE5 is described for the first time. Arm F comprises three previously described sequences: tendF1, tendF3, and tendF4. In chromosome II (CDG), sequence tendC1 occurs in most of the studied larvae, while tendC2 and tendC3 are rare sequences. Three previously described sequences, tend(DG)1, tend(DG)2, and tend(DG)3, occur in arm DG; the band order of the previously described sequence tend(DG)4 is clarified. Two new sequences, tend(DG)5 and tend(DG)6, are described for the first time. In chromosome III (AB), arm A comprises four known sequences: tendA1, tendA2, tendA3, and tendA4. Arm B comprises only two sequences: tendB1 and tendB2. In total, 24 chromosome banding sequences have been discovered in the studied E. tendens populations.
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Belyanina, S.I., Larval karyotypes of Endochironomus tendens F. from different regions of the Volga River, Tsitologiya, 1978, vol. 20, no. 5, p. 596.
Belyanina, S.I., Karyotypic analysis of chironomids (Diptera, Chironomidae) in the Fauna of the USSR, Doctoral Dissertation in Biology, Moscow, 1983.
Belyanina, S.I., The karyotype of the Volga chironomid Demeijerea rufipes L., an endoparasite of sponges, in Kariosistematika bespozvonochnykh zhivotnykh II. Sbornik nauchnykh trudov (Karyosystematics of Invertebrates II: Collected Papers), St. Petersburg, 1993, p. 55.
Belyanina, S.I. and Durnova, N.A., Morphology and chromosomes of phytophilous Glyptotendipes glaucus (Diptera, Chironomidae) from reservoirs of Saratov Region: 2. Karyotypic analysis, Zool. Zh., 1998, vol. 78, no. 12, p. 243.
Bolshakov, V.V. and Shobanov, N.A., The effects of microenvironmental conditions on the cytogenetic population structure of Chironomus plumosus in the Rybinsk Reservoir, Tr. Inst. Biol. Vnutr. Vod, 2017, vol. 80, p. 20.
Demin, S.Yu. and Shobanov, N.A., Karyotype of Chironomus entis of the plumosus group in the European part of the USSR, Tsitologiya, 1990, vol. 32, no. 10, p. 1046.
Durnova, N.A., Chromosome polymorphism and cytotypes of Endochironomus tendens F. (Diptera, Chironomidae) from Saratov and Samara regions, Tsitologiya, 2009a, vol. 51, no. 7, p. 592.
Durnova, N.A., Chromosome analysis of chironomids (Diptera: Chironomidae) – obligatory miners of freshwater sponges (Porifera), Comp. Cytogenet., 2009b, vol. 3, no. 1, p. 25.
Durnova, N.A., Cytogenetic characteristics of Stenochironomus gibbus (Fabricius, 1794) (Diptera, Chironomidae), an obligate miner of submerged wood, Tsitologiya, 2010a, vol. 52, no. 7, p. 883.
Durnova, N.A., Chironomids in the periphyton of Saratov Province: ecological features, morphology, and cytogenetics (Diptera, Chironomidae, Chironomini), Extended Abstract of Doctoral Dissertation in Biology, St. Petersburg: Zool. Inst., 2010b.
Durnova, N.A. and Oglezneva, A.A., Recent data on chromosome polymorphism of phytophilous Glyptotendipes mancunianus Mg. (Diptera, Chironomidae) from different reservoirs of the Saratov Province, in Entomologicheskie i parazitologicheskie issledovaniya v Povolzh’e. Sbornik nauchnykh trudov (Entomological and Parasitological Research in the Volga Basin: Collected Papers), Issue 12, Saratov: Saratov Univ., 2015, p. 27.
Durnova, N.A., Voronin, M.Yu., and Sukhova, E.I., Biotopic distribution of periphytic Chironomidae in water reservoirs of Saratov region, Povolzh. Ekol. Zh., 2011, vol. 3, p. 304.
Durnova, N.A., Voronin, M.Yu., and Oglezneva, A.A., Selectivity of settling certain types of macrophytes by chironomid miners (Diptera, Chironomidae), in Entomologicheskie i parazitologicheskie issledovaniya v Povolzh’e. Sbornik nauchnykh trudov (Entomological and Parasitological Research in the Volga Basin: Collected Papers), Issue 10, Saratov: Saratov Univ., 2012, p. 55.
Durnova, N.A., Voronin, M.Yu., and Oglezneva, A.A., Chromosomal polymorphism and cytogenetic divergence o Glyptotendipes glaucus (Meigen) (Diptera: Chironomidae) in the reservoirs of Saratov Province, Tr. Russ. Entomol. Ob-va, 2014, vol. 85, no. 2, p. 19.
Durnova, N.A., Sigareva, L., and Sinichkina, O., Chromosome analysis of Endochironomus albipennis Meigen, 1830 and morphologically similar Endochironomus sp. (Diptera, Chironomidae) from water bodies of the Volga region, Russia, Comp. Cytogenet., 2015, vol. 9, no. 4, p. 579.
Gunderina, L.I., Aimanova, K.G., Proviz, V.I., Rakisheva, A.Zh., Kiknadze, I.I., Istomina, A.G., Salova, T.A., and Butler, M.G., Cytogenetic differentiation of natural and laboratory populations of Camptochironomus tentans (Fabricius) (Chironomidae: Diptera), Genetika, 1996, vol. 32, p. 53.
Kiknadze, I.I., The role of chromosomal polymorphism in divergence of populations and species of the genus Chironomus (Diptera), Zool. Zh., 2008, vol. 87, no. 6, p. 686.
Kiknadze, I.I., Butler, M.G., Aimanova, K.G., Gunderina, L.I., and Cooper, J.K., Geographic variation in the polytene chromosome banding pattern of the Holarctic midge Chironomus (Camptochironomus) tentans (Fabricius), Can. J. Zool., 1996, vol. 74, no. 1, p. 171.
Kiknadze, I.I., Michailova, P., Istomina, A.G., Golygina, V.V., Int Panis, L., and Krastanov, B., The chromosomal polymorphism and divergence of populations in Chironomus nuditarsis Str. (Diptera, Chironomidae), Tsitologiya, 2006, vol. 48, no. 7, p. 595.
Makarchenko, E.A. and Makarchenko, M.A., Family Chironomidae: non-biting midges, in Opredelitel’ nasekomykh Dal’nego Vostoka Rossii (Key to Insects of the Russian Far East), Vol. 6, Part 4: Dvukrylye i blokhi (Diptera and Siphonaptera), Vladivostok: Dalnauka, 2006, p. 204.
Michailova, P.V., Comparative karyological analysis of species of genus Endochironomus Kieff., Entomology, 1987, vol. 29, p. 105.
Michailova, P.V., The polytene chromosomes and their significance to the systematic and phylogeny of the family Chironomidae, Diptera, Acta Zool. Fenn., 1989, vol. 186, p. 1.
Michailova, P.V., Endochironomus tendens (F.) (Diptera, Chironomidae) an example of stasipatric speciation, Neth. J. Aquat. Ecol., 1992, vol. 26, p. 173.
Michailova, P.V. and Gercheva, P., Cytotaxonomical characteristics of the genus Endochironomus Kieff. (Diptera, Chironomidae), Caryologia, 1982, vol. 35, p. 33.
Petrova, N., Michailova, P., and Bovero, S., Cytogenetic characteristics of Chironomus nuditarsis Str. (Chironomidae, Diptera) and its relationship with species from the plumosus group, in Late 20th Century Research on Chironomidae: an Anthology from the 13th Int. Symp. on Chironomidae, Freiburg, 5–9 September 1997, Aachen: Shaker, 2000, p. 201.
Shobanov, N.A., The karyotype pool of Chironomus plumosus L. (Diptera, Chironomidae). Intra- and interpopulation polymorphism, Tsitologiya, 1994a, vol. 36, no. 11, p. 1129.
Shobanov, N.A., The karyotype pool of Chironomus plumosus L. (Diptera, Chironomidae). III. The dependence of the karyotype pool parameters on the sample size and the population structure, Tsitologiya, 1994b, vol. 36, no. 3, p. 289.
Vinokurova, N.V., Kalinina, E.A., and Stol, E.E., Karyotype and inversion polymorphism of natural populations Glyptotendipes glaucus Meigen, 1818 (Diptera, Chironomidae) from the small reservoirs of Kaliningrad, Ecol. Genet., 2016, vol. 14, no. 4, p. 41.
The authors declare that they have no conflict of interest. All the applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All the procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
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Oglezneva, A.A., Durnova, N.A. Populational and Karyological Analysis of the Phytophilous Chironomid Endochironomus tendens F. (Diptera, Chironomidae). 1. New Chromosomal Sequences in the Species’ Karyotype Pool. Entmol. Rev. 100, 982–992 (2020). https://doi.org/10.1134/S0013873820070039
- polytene chromosome
- chromosomal sequence
- Endochironomus tendens