Abstract—Using Raman spectroscopy (RS) approach in a spectral range of 1000–3000 cm–1 were used to study the conformational and structural changes that arise in the heme group and globin moiety of hemoglobin in human red blood cells at various temperatures and oxygen contents. In hypoxia, the hemoglobin conformation was shown to change as a result of the increasing contribution of hematoporphyrin pyrrole rings and vibrational motions of vinyl groups. Modifications were additionally detected in the contributions of symmetric and asymmetric vibrations of the CH2 and CH3 radicals of histidine (2850, 2860, and 2900 cm–1) and lysine (2880 and 2860 cm–1) residues. The mechanisms of oxygen binding are discussed for hemoglobin located in the submembrane region and cytoplasm of the cell.
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Abbreviations: Hb, hemoglobin; RS, Raman spectroscopy.
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Slatinskaya, O.V., Luneva, O.G., Deev, L.I. et al. Conformational Changes that occur in Heme and Globin upon Temperature Variations and Normobaric Hypoxia. BIOPHYSICS 65, 213–221 (2020). https://doi.org/10.1134/S0006350920020220
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DOI: https://doi.org/10.1134/S0006350920020220