Abstract—Using Raman spectroscopy (RS) approach in a spectral range of 1000–3000 cm–1 were used to study the conformational and structural changes that arise in the heme group and globin moiety of hemoglobin in human red blood cells at various temperatures and oxygen contents. In hypoxia, the hemoglobin conformation was shown to change as a result of the increasing contribution of hematoporphyrin pyrrole rings and vibrational motions of vinyl groups. Modifications were additionally detected in the contributions of symmetric and asymmetric vibrations of the CH2 and CH3 radicals of histidine (2850, 2860, and 2900 cm–1) and lysine (2880 and 2860 cm–1) residues. The mechanisms of oxygen binding are discussed for hemoglobin located in the submembrane region and cytoplasm of the cell.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
D. Holly and B. Glenn, Am. J. Physiol. Heart Circ. Physiol. 293, 2193 (2007)
M. R. Hardeman, P. T. Goedhart, and S. K. Shin, in Handbook of Hemorheology and Hemodynamics (IOS Press Ebooks, 2007), pp. 242–266.
J. A. Walder, R. Chatterjee, T. L. Steck, et al., J. Biol. Chem. 259 (16), 10238 (1984).
R. A. Reithmeier, J. R. Casey, A. C. Kalli, et al., Biochim. Biophys. Acta–Biomembr. 1858 (7), 1507 (2016
R. R. Tuck, J. D. Schmelzer, and P. A. Low, Brain 107 (3), 935 (1984)
O.G. Luneva, S. V. Sidorenko, O. O. Ponomarchuk, et al., Cell. Physiol. Biochem. 39 (1), 81 (2016)
S. V. Sidorenko, O. G. Luneva, T. S. Novozhilova, et al., Biochemistry, Suppl. Ser. A 12 (2), 114 (2018)
N. A. Brazhe, S. Abdali, A. R. Brazhe, et al., Biophys. J. 97 (12), 3206 (2009)
B. R. Wood and D. McNaughton, Biopolymers 67 (4–5), 1691 (2002)
G. V. Maksimov, N. V. Maksimova, A. A. Churin, et al., Biochemistry (Moscow) 66 (3), 295 (2001)
S. C. Goheen, L. J. Lis, O. Kucuk, et al., J. Raman Spectrosc. 24 (9), 275 (1993)
N. Parthasarathi, C. Hansen, S. Yamaguchi, et al., J. Am. Chem. Soc. 109 (13), 3865 (1987)
B. R. Wood, P. Caspers, G. J. Puppels, et al., Anal. Bioanal. Chem. 387 (5), 1691 (2007)
S. Nagatomo, M. Nagai, Y. Mizutani, et al., Biophys. J. 89 (2), 1203 (2005)
S. Choi, T. G. Spiro, K. C. Langry, et al., J. Am. Chem. Soc. 104 (16), 4345 (1982)
N. K. Howell, G. Arteaga, S. Nakai, et al., J. Agricult. Food Chem. 47 (3), 924 (1999)
H. Lowry, N. J. Rosebrough, A. L. Farr, et al., J. Biol. Chem. 193, 265 (1951)
S. V. Sidorenko, R. H. Ziganshin, O. G. Luneva, et al., J. Proteomics 184, 25 (2018)
I. P. Torres Filho, J. Terner, R. N. Pittman, et al., J. Appl. Physiol. 104 (6), 1809 (2008)
I. A. Tikhomirova, A. V. Murav’ev, L. A. Mikhailichenko, et al., Human Physiol. 32 (6), 748 (2006)
K. M. Marzec, D. Perez-Guaita, M. De Veij, et al., ChemPhysChem 15 (18), 3963 (2014)
B. R. Wood, L. Hammer, and D. McNaughton, Vib. Spectrosc. 38 (1-2), 78 (2005)
J. Surmacki, R. Musial, R. Kordek, et al., Mol. Cancer 12 (1), 48 (2013)
M. Nagai, N. Mizusawa, T. Kitagawa, et al., Biophys. Rev. 10 (2), 271 (2018)
J. M. Friedman, T. W. Scott, R. A. Stepnoski, et al., J. Biol. Chem. 258 (17), 10564 (1983)
S. Nagatomo, M. Nagai, and T. Kitagawa, J. Am. Chem. Soc. 133 (26), 10101 (2011)
S. N. Orlov, Byull. Sib. Med. 18 (2), 234 (2019)
A. A. Semenova, A. P. Semenov, E. A. Gudilina, et al., Mendeleev Commun. 26 (3), 177 (2016).
Conflict of interest. The authors declare that they have no conflict of interest.
Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the 1964 Helsinki Declaration and its later amendments. Informed consent was voluntarily provided by all individual participants involved in the study.
Translated by T. Tkacheva
Abbreviations: Hb, hemoglobin; RS, Raman spectroscopy.
About this article
Cite this article
Slatinskaya, O.V., Luneva, O.G., Deev, L.I. et al. Conformational Changes that occur in Heme and Globin upon Temperature Variations and Normobaric Hypoxia. BIOPHYSICS 65, 213–221 (2020). https://doi.org/10.1134/S0006350920020220
- Keywords: hemoglobin
- red blood cells
- Raman spectroscopy