Oxidative Modification of Proteins in the Tissues of Rats with Growing Tumors under the Ozone-Photodynamic Treatment

Abstract—Ozone-photodynamic effects on tumor growth rate and oxidative modification of proteins in rat tissues were studied using a model of PA kidney carcinoma. It was shown that the developed experimental therapeutic method based on the joint parenteral intraperitoneal administration of an ozonated saline solution with a concentration of ozone in the ozone–oxygen mixture of 400 μg/L and a 0.3% solution of the Photosens photosensitizer intratumorally with the local action of light with a wavelength of 660 ± 10 nm for 10 min reduced the intensity of tumor growth. Normalization of the prooxidant/antioxidant balance in experimental animals was observed upon combined use of ozonated saline solution and photodynamic therapy.

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Funding

The research was carried out with the financial support of the Russian Foundation for Basic Research, project no. 19-02-00667.

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Correspondence to T. G. Shcherbatyuk.

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CONFLICT OF INTEREST

The authors declare no conflict of interest.

COMPLIANCE WITH ETHICAL STANDARDS

All manipulations with animals were carried out in accordance with the recommendations established by the European Convention for the protection of vertebrates used for experimental and other scientific purposes (Strasbourg, 2006), and in accordance with the Helsinki Declaration on the humane treatment of animals (Edinburgh, 2000). The work was approved by the Local Ethics Committee of PRMU.

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Translated by E. Puchkov

Abbreviations: PDT, photodynamic therapy; OPS, ozonated physiological saline; OMP, oxidative modification of proteins; DNPH, dinitrophenylhydrazones; TBA, thiobarbituric acid; EB, exploratory behavior.

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Shcherbatyuk, T.G., Zhukova (Plekhanova), E.S., Nikitina, J.V. et al. Oxidative Modification of Proteins in the Tissues of Rats with Growing Tumors under the Ozone-Photodynamic Treatment. BIOPHYSICS 65, 319–326 (2020). https://doi.org/10.1134/S0006350920020219

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  • Keywords: photodynamic effect
  • ozonated physiological saline
  • oxidative stress
  • oxidative modification of proteins
  • experimental kidney carcinoma
  • outbred rats