The Functional Activity of Mitochondria in Deuterium Depleted Water

Abstract—The efficiency of phosphorylation in samples of isolated rat liver mitochondria in incubation media with variable isotopic composition at deuterium contents of 6, 60, 90, 120, 150 ppm, as well as 0.1, 1 and 2%, was studied. In media with a low deuterium content (up to 60 ppm), a monotonic decrease in the ADP/O ratio was observed, while at 6 ppm its value was the same as at 60 ppm. The generation of superoxide anion by complex I, II, and III of the mitochondrial respiratory chain was also investigated. For all studied modes of mitochondrial functioning using respiratory chain inhibitors, the generation of superoxide anion was independent of the deuterium concentration in the incubation medium.

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ACKNOWLEDGMENTS

The authors thank L. S. Yaguzhinskii for useful discussions.

Funding

Part of this work (measurement of mitochondrial respiration) was financially supported by the Russian Foundation for Basic Research (project no. 19-04-00835\19)

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Correspondence to V. I. Lobyshev.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All procedures for the maintenance and use of animals were in accordance with the standards of the International Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC).

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Translated by D. Novikova

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Lobysheva, N.V., Nesterov, S.V., Skorobogatova, Y.A. et al. The Functional Activity of Mitochondria in Deuterium Depleted Water. BIOPHYSICS 65, 272–276 (2020). https://doi.org/10.1134/S0006350920020128

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  • Keywords: mitochondria
  • electron transport
  • inhibitors
  • superoxide anion
  • hydrogen peroxide
  • phosphorylation
  • water deuterium
  • isotopic effects