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Expression of mRNAs for IL-1β, IL-6, IL-10, TNFα, CX3CL1, and TGFβ1 Cytokines in the Brain Tissues: Assessment of Contribution of Blood Cells with and without Perfusion

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Abstract

Cytokines are important regulators of brain function under both normal and pathological conditions. Cytokines can be synthesized by resident cells of the central nervous system (CNS) (vascular endothelium, cells of the blood-brain barrier, parenchymal cells of the CNS) or cells in the lumen of blood vessels, as well as introduced with the bloodstream. The ratio between the quantity of cytokines synthesized in the CNS and those entering it from external sources under various conditions remains poorly understood. In this work, we studied the contribution of mRNAs from non-resident cells to the common pool of cytokine (TNFα, IL-1β, IL-6, IL-10, CX3CL1, and TGFβ1) mRNAs in the rat neocortex, hippocampus, dura matter, pia matter, and choroid plexus. We also evaluated the representation of various populations of resident and non-resident immune cells based on the expression of marker genes (Ncf1, Tbx21, Foxp3, RORγc). The removal of blood by transcardial perfusion led to a decrease in the quantity of the TNFα mRNA in the neocortex and hippocampus and of the IL-1β, IL-6, and IL-10 mRNAs in the dura mater. The mRNA levels of other cytokines in studied structures were not affected by perfusion. Our findings suggest that mRNAs present in the blood can make a significant contribution to the mRNA levels of some cytokines in the CNS; therefore, preliminary perfusion of brain tissue is a necessary stage of experimental design for correct estimation of mRNA content in the brain.

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Abbreviations

CX3CL1:

C-X3-C chemokine ligand 1 (fractalkine)

IL:

interleukin

TNFα:

tumor necrosis factor alpha

TGFβ1:

transforming growth factor beta 1

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Funding

Funding. This work was supported by the Russian Foundation for Basic Research (project 18-015-00314a).

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia

    A. A. Kvichansky, M. N. Volobueva, Yu. S. Spivak, L. V. Tret’yakova, N. V. Gulyaeva & A. P. Bolshakov

Authors
  1. A. A. Kvichansky
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  2. M. N. Volobueva
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  3. Yu. S. Spivak
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  4. L. V. Tret’yakova
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  5. N. V. Gulyaeva
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  6. A. P. Bolshakov
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Corresponding author

Correspondence to N. V. Gulyaeva.

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Conflict of interest. The authors declare no conflict of interest.

Ethical approval. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The experimental protocol was approved by the Ethics Committee of the Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences.

Additional information

Published in Russian in Biokhimiya, 2019, Vol. 84, No. 8, pp. 1147–1153.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM19–086, June 10, 2019.

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Kvichansky, A.A., Volobueva, M.N., Spivak, Y.S. et al. Expression of mRNAs for IL-1β, IL-6, IL-10, TNFα, CX3CL1, and TGFβ1 Cytokines in the Brain Tissues: Assessment of Contribution of Blood Cells with and without Perfusion. Biochemistry Moscow 84, 905–910 (2019). https://doi.org/10.1134/S0006297919080066

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  • DOI: https://doi.org/10.1134/S0006297919080066

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