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Identification of messenger RNA substrates for mouse T-STAR

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Abstract

Using the method of isolation of specific nucleic acids associated with proteins (SNAAP), we have identified 10 candidate target mRNA substrates bound by mT-STAR (mouse T-STAR protein) from testis extract. Among them, our study focused on Fabp9, a gene that is essential for male gametogenesis, and showed that mT-STAR could directly bind to Fabp9 mRNAs. The binding sites are in a short sequence of the coding region and 3′ untranslated region of Fabp9 mRNA. These suggest that mT-STAR can regulate the metabolism and expression of Fabp9. In conclusion, identification of mT-STAR-bound mRNA substrates might help to illustrate the potential spectrum of the process and provide valuable insight into the biological function of this RNA-binding protein in spermatogenesis.

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Abbreviations

DIG:

digoxigenin

hnRNP:

heterogeneous nuclear ribonucleoprotein

mT-STAR:

mouse T-STAR (testis signal transduction and activation of RNA) protein

RBB:

RNA binding buffer

SNAAP:

specific nucleic acids associated with proteins

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Authors and Affiliations

  1. Department of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, P. R. China

    L. Y. Zhang, M. Zeng, P. Chen, H. Q. Sun, D. C. Tao, Y. Q. Liu, L. Lin, Y. Yang, S. Z. Zhang & Y. X. Ma

Authors
  1. L. Y. Zhang
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  2. M. Zeng
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  3. P. Chen
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  4. H. Q. Sun
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  5. D. C. Tao
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  6. Y. Q. Liu
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  7. L. Lin
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  8. Y. Yang
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  9. S. Z. Zhang
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Correspondence to Y. X. Ma.

Additional information

Published in Russian in Biokhimiya, 2009, Vol. 74, No. 11, pp. 1560–1568.

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Zhang, L.Y., Zeng, M., Chen, P. et al. Identification of messenger RNA substrates for mouse T-STAR. Biochemistry Moscow 74, 1270–1277 (2009). https://doi.org/10.1134/S0006297909110145

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  • DOI: https://doi.org/10.1134/S0006297909110145

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