Obtainment of a Complex Enzyme Preparation with Enhanced Pectinase Activity Based on the New Mutant Strain T. reesei Co-44


Studies were conducted to obtain a complex enzyme preparation that allows efficient hydrolysis of the main plant nonstarch polysaccharides (cellulose, xylans, and pectin). The goal of the studies was to optimize the composition of a fermentation medium for the submerged cultivation of the new mutant strain T. reesei Co-44, a highly active producer of endo-carbohydrases. A concentrate of low molecular soy components was chosen as the inducer of the key carbohydrase biosynthesis by the strain. This concentrate provided the maximum (more than eightfold) increase in polygalacturonase activity and an increased level of endoglucanase and xylanase biosynthesis. After the cultivation of T. reesei Co-44 under optimized conditions, a complex enzyme preparation, Xylorizin K4, was obtained, and its physicochemical properties were studied. The presence of endopolygalacturonase (GH28) T. reesei in Xylorizin K4 was confirmed via electrophoresis and MALDI⎯TOF mass spectrometry. The studies show the potential of Xylorizin K4 application for the production of soy-protein concentrates to eliminate the main soybean nonstarch polysaccharides.

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  1. 1

    Mikhailova, R.V., Matseriruyushchie fermenty mitselial’nykh gribov v biotekhnologii (Macerating Enzymes of Filamentous Fungi in Biotechnology), Minsk: Belorusskaya nauka, 2007.

  2. 2

    Bedford, M.R. and Partridge, G.G., Enzymes in Farm Animal Nutrition, Wallingford, Oxforshire, UK, Cambridge, MA: CAB Int., 2010.

  3. 3

    Sajith, S., Priji, P., Sreedevi, S., and Benjamin, S., J. Nutr. Food Sci., 2016, vol. 6, p. 461. https://doi.org/10.4172/2155-9600.1000461

    CAS  Article  Google Scholar 

  4. 4

    Herpoël-Gimbert, I., Margeot, A., Dolla, A., Jan, G., Molle, D., Lignon, S., Mathis, H., Sigoillot, J.-C., Monot, F., and Asther, M., Biotechnol. Biofuels, 2008, vol. 1, p. 18. https://doi.org/10.1186/1754-6834-1-1

    Article  PubMed  PubMed Central  Google Scholar 

  5. 5

    Druzhinina, I.S. and Kubicek, C.P., Microb. Biotechnol., 2017, vol. 10, no. 6, pp. 1485–1499. https://doi.org/10.1111/1751-7915.12726

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. 6

    Payne, C.M., Knott, B.C., Mayes, H.B., Hansson, H., Himmel, M.E., Sandgren, M., Ståhlberg, J., and Beckham, G.T., Chem. Rev., 2015, vol. 115, no. 3, pp. 1308–448. https://doi.org/10.1021/cr500351c

    CAS  Article  PubMed  Google Scholar 

  7. 7

    Markovič, O., Slezárik, A., and Labudová, I., FEMS Microbiol. Lett., 1985, vol. 27, no. 3, pp. 267–271. https://doi.org/10.1111/j.1574-6968.1985.tb00680.x

    Article  Google Scholar 

  8. 8

    Olsson, L., Christensen, T.M.I.E., Hansen, K.P., and Palmqvist, E.A., Enzyme Microbial Technol., 2003, vol. 33, no. 5, pp. 612–619. https://doi.org/10.1016/S0141-0229(03)00181-9

  9. 9

    Mohamed, S.A., Christensen, T.M.I.E., and Mikkelsen, J.D., Carbohydr. Res., 2003, vol. 338, no. 6, pp. 515–524.

    CAS  Article  Google Scholar 

  10. 10

    Saloheimo, M. and Pakula, T., Microbiology, 2012, vol. 158, no. 1, pp. 46–57. https://doi.org/10.1099/mic.0.053132-0

    CAS  Article  PubMed  Google Scholar 

  11. 11

    Pedrolli, D.B., Monteiro, A.P., Gomes, E.L., and Carmona, E.C., Open Biotechnol. J., 2009, vol. 3, pp. 9–18. https://doi.org/10.2174/1874070700903010009

    CAS  Article  Google Scholar 

  12. 12

    Tapre, A. and Jain, R.K., Int. Food Res. J., 2014, vol. 21, no. 2, pp. 447–453.

    CAS  Google Scholar 

  13. 13

    Kostyleva, E.V., Tsurikova, N.V., Sereda, A.S., Velikoretskaya, I.A., Veselkina, T.N., Lobanov, N.S., Shashkov, I.A., and Sinitsyn, A.P., Microbiology, 2018, vol. 87, pp. 652–661. https://doi.org/10.1134/S0026261718050120

    CAS  Article  Google Scholar 

  14. 14

    Vanitha, T. and Khan, M., Role of pectin in food processing and food packaging, in Pectins—Extraction, Purification, Characterization and Applications, 2019. https://doi.org/10.5772/intechopen.83677

  15. 15

    Collmer, A., Reid, J., and Mount, M., Methods Enzymol., 1988, vol. 161, pp. 329–335.

    CAS  Article  Google Scholar 

  16. 16

    Sinitsyn, A.P., Gusakov, A.V., and Chernoglazov, V.A., Biokonversiya lignotsellyuloznykh materialov (Bioconversion of Lignocellulosic Materials), Moscow: Mosk. Gos. Univ., 1995.

  17. 17

    Hao, X.-C., Yu, X.-B., and Yan, Zh.-L., Food Technol. Biotechnol., 2006, vol. 44, no. 1, pp. 89–94.

    CAS  Google Scholar 

  18. 18

    Saravanan, P., Muthuvelayudham, R., and Viruthagiri, T., Enzyme Res., 2012, vol. 2012, p. 7. https://doi.org/10.1155/2012/157643

    CAS  Article  Google Scholar 

  19. 19

    Khabibulina, N.V., Bikbov, T.M., and Ponomarev, V.V., Proekt. Kul’t. Kach. Zhizni, 2015, no. 1, pp. 473–491.

  20. 20

    Sundarraj, A.A. and Ranganathan, T.V., Int. J. Appl. Environ. Sci., 2017, vol. 12, no. 10, pp. 1777–1801.

    Google Scholar 

  21. 21

    Barnes, M., Brown, M., Rosentrater, K., and Sewell, J., Open J. Anim. Sci., 2012, vol. 2, no. 4, pp. P. 234–243. https://doi.org/10.4236/ojas.2012.24033

  22. 22

    Delgado, E. and Reyes-Jaquez, D., Extrusion of Metals, Polymers and Food Products, 2017. https://doi.org/10.5772/65577

    Google Scholar 

  23. 23

    Karr-Lilienthal, L.K., Kadzere, C.T., Grieshop, C.M., and Fahey, G.C., Livestock Prod. Sci., 2005, vol. 97, no. 1, pp. 1–12.

    Article  Google Scholar 

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The equipment of the Industrial Biotechnology Center for Collective Use of the Fundamentals of Biotechnology Federal Research Center of the Russian Academy of Sciences was used in this work.


This work was carried out within the framework of the Program of Basic Scientific Research of the State Academies of Sciences for 2019–2021 (topic no. 0529-2019-0066).

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Correspondence to E. V. Kostyleva.

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The authors declare that they have no conflict of interest. This article does not contain any research involving humans or animals as research objects.

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Translated by P. Kuchina

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Kostyleva, E.V., Sereda, A.S., Velikoretskaya, I.A. et al. Obtainment of a Complex Enzyme Preparation with Enhanced Pectinase Activity Based on the New Mutant Strain T. reesei Co-44. Appl Biochem Microbiol 57, 94–101 (2021). https://doi.org/10.1134/S0003683821010130

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  • Trichoderma reesei
  • polygalacturonase
  • endoglucanase
  • xylanase
  • nonstarch polysaccharides
  • fermentation medium