Prospects for the Use of Methylotrophic Yeast in the Creation of Industrial Producers of Feed Enzymes


In recent years, mycelial fungi have faced competition from recombinant yeast as producers of feed enzymes. An intensive study on genetic diversity identified the yeast genes encoding feed enzymes, the specific activity of which is much higher than that in mycelial fungi. In addition, these genes were expressed in yeast much more efficiently than in mycelial fungi. The use of yeast recombinant producers allowed the expansion of the production of a line of industrial enzymes with a significant reduction in their cost. The advantages of yeast producers of recombinant enzymes include the ability to obtain monoenzymes, which are part of various enzyme complexes used for different purposes. Pichia pastoris methylotrophic yeast is the most attractive subject for the creation of recombinant protein-producing strains.

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The work was carried out at the Multipurpose Scientific Installation of the All-Russia Collection of Industrial Microorganisms of the Kurchatov Institute National Resource Center.


The work was carried out with the financial support of the Ministry of Education and Science of Russia (Unique Project Identifier RFMEFI60717X0180).

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Correspondence to O. E. Mel’kina.

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This article does not contain any studies involving animals performed by any of the authors.

This article does not contain any studies involving human participants performed by any of the authors.

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Translated by I. Gordon

Abbreviations: CL—culture liquid; NSP—nonstarch polisaccharides; UPR—unfolded protein response.

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Mel’kina, O.E., Sineoky, S.P. Prospects for the Use of Methylotrophic Yeast in the Creation of Industrial Producers of Feed Enzymes. Appl Biochem Microbiol 56, 815–821 (2020).

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  • feed enzymes
  • Pichia pastoris
  • phytase
  • xylanase
  • β-glucanase
  • AOX1 promoter