Identification of Bacteria by Surface-Enhanced Raman Spectra after Peroxide Treatment
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Surface-enhanced Raman spectroscopy (SERS) is a prospective method for the detection and identification of microorganisms. Hereby we describe the method for the registration of bacterial SERS spectra, including the mild oxidation sample treatment for the additional amplification of SERS signal. We also propose the spectral data processing method for the calculation of distinguishing criteria for the bacterial spectra.
Keywords:gold nanoparticles principal component analysis surface-enhanced Raman scattering identification of bacteria
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
- 10.Premasiri, W.R., Moir, D.T., Klempner, M.S., Krieger, N., Jones, G., and Ziegler, L.D., J. Phys. Chem. B, vol. 109, no. 1, pp. 312–320.Google Scholar
- 11.Lee, S.J., Guan, Z., Xu, H., and Moskovits, M., J. Phys. Chem., vol. 111, no. 49, pp. 17985–17988.Google Scholar
- 12.Leopold, N. and Lendl, B., J. Phys. Chem. B, vol. 107, no. 24, pp. 5723–5727.Google Scholar