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Applied Biochemistry and Microbiology

, Volume 53, Issue 2, pp 165–172 | Cite as

PEGylated recombinant L-asparaginase from Erwinia carotovora: Production, properties, and potential applications

  • N. S. Melik-Nubarov
  • I. D. Grozdova
  • G. Yu. Lomakina
  • M. V. Pokrovskaya
  • V. S. Pokrovski
  • S. S. Aleksandrova
  • O. Yu. Abakumova
  • O. V. Podobed
  • D. V. Grishin
  • N. N. Sokolov
Article

Abstract

N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of the enzyme with a 25-fold excess of reagent relative to the enzyme tetramer. The modified L-asparaginase was isolated from the reaction mixture by gel filtration on Sepharose CL-6B. The purified bioconjugate did not contain PEG unbound to the protein, demonstrated high catalytic activity, and exhibited antiproliferative action on cell cultures.

Keywords

recombinant L-asparaginase from Erwinia carotovora PEG-asparaginase polyethylene glycol antitumor therapeutics 

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Copyright information

© Pleiades Publishing, Inc. 2017

Authors and Affiliations

  • N. S. Melik-Nubarov
    • 1
  • I. D. Grozdova
    • 1
  • G. Yu. Lomakina
    • 1
  • M. V. Pokrovskaya
    • 2
  • V. S. Pokrovski
    • 2
  • S. S. Aleksandrova
    • 2
  • O. Yu. Abakumova
    • 2
  • O. V. Podobed
    • 2
  • D. V. Grishin
    • 2
  • N. N. Sokolov
    • 2
  1. 1.Department of ChemistryLomonosov Moscow State UniversityMoscowRussia
  2. 2.Orekhovich Scientific Research Institute of Biomedical ChemistryMoscowRussia

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