Applied Biochemistry and Microbiology

, Volume 53, Issue 2, pp 258–266 | Cite as

Effect of lipopolysaccharide O-side chains on the adhesiveness of Yersinia pseudotuberculosis to J774 macrophages as revealed by optical tweezers

  • A. A. Byvalov
  • V. L. Kononenko
  • I. V. Konyshev


A method has been developed for the quantitative estimation of the binding force of a model microsphere with a eukaryocyte based on the optical trap in order to study the molecular mechanism of adhesion between an individual bacterium and a host cell. The substantial role of LPS O-side chains in the adhesiveness of Yersinia pseudotuberculosis 1b to J774 macrophages has been revealed with the use of a set of microspheres functionalized with lipopolysaccharide (LPS) preparations and antibodies with different specificities. The results indicate the significance of the O-antigen as a pathogenicity factor of Y. pseudotuberculosis in colonization of a macroorganism. The developed methodical approaches can be applied to the study of molecular mechanisms of the pathogenesis of pseudotuberculosis and other infectious diseases to improve antiepidemic service.


Yersinia pseudotuberculosis lipopolysaccharide O-side chains monoclonal antibodies optical tweezers microspheres adhesion 


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  1. 1.
    Kline, K.A., Falker, S., Dahlberg, S., Normark, S., and Henriques-Normark, B., Cell Host Microbe, 2009, vol. 5(6), pp. 580–592.CrossRefPubMedGoogle Scholar
  2. 2.
    Zav’yalov, V., Zavialov, A., Zav’yalova, G., and Korpela, T., FEMS Microbiol. Rev., 2010, vol. 34(3), pp. 317–378.CrossRefPubMedGoogle Scholar
  3. 3.
    Mikula, K.M., Kolodziejczyk, R., and Goldman, A., Front. Cell. Infect. Microbiol., 2012, vol. 2, pp. 1–14.Google Scholar
  4. 4.
    Pizarro-Cerdá, J. and Cossart, P., Cell, 2006, vol. 124(4), pp. 715–727.CrossRefPubMedGoogle Scholar
  5. 5.
    Razatos, A., Ong, Y.L., Sharma, M.M., and Georgiou, G., Proc. Natl. Acad. Sci. U. S. A., 1998, vol. 95(19), pp. 11059–11064.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Boyle, E.C. and Finlay, B.B., Curr. Opin. Cell. Biol., 2003, vol. 15(5), pp. 633–639.CrossRefPubMedGoogle Scholar
  7. 7.
    Krachler, A.M. and Orth, K., Microbe, 2013, vol. 8, pp. 286–290.Google Scholar
  8. 8.
    Miller, V.L., Beer, K.B., Heusipp, G., Young, B.M., and Wachtel, M.R., Mol. Microbiol., 2001, vol. 41(5), pp. 1053–1062.CrossRefPubMedGoogle Scholar
  9. 9.
    Yang, Y., Merriam, J.J., Mueller, J.P., and Isberg, R.R., Infect. Immun., 1996, vol. 64(7), pp. 2483–2489.PubMedPubMedCentralGoogle Scholar
  10. 10.
    Felek, S., Lawrenz, M.B., and Krukonis, E.S., Microbiology, 2008, vol. 154(6), pp. 1802–1812.CrossRefPubMedGoogle Scholar
  11. 11.
    Lawrenz, M.B., Lenz, J.D., and Miller, V.L., Infect. Immun., 2009, vol. 77(1), pp. 317–326.CrossRefPubMedGoogle Scholar
  12. 12.
    Nair, M.K.M., De Masi, L., Yue, M., Galván, E.M., Chen, H., Wang, F., and Schifferli, D.M., Infect. Immun., 2015, vol. 83(5), pp. 1809–1819.CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Eitel, J. and Dersch, P., Infect. Immun., 2002, vol. 70(9), pp. 4880–4891.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Isberg, R.R., Voorhis, D.L., and Falkow, S., Cell, 1987, vol. 50(5), pp. 769–778.CrossRefPubMedGoogle Scholar
  15. 15.
    Felek, S., Tsang, T.M., and Krukonis, E.S., Infect. Immun., 2010, vol. 78(8), pp. 4134–4150.CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Lahteenmaki, K., Kukkonen, M., and Korhonen, T.K., FEBS Lett., 2001, vol. 504(1-2), pp. 69–72.CrossRefPubMedGoogle Scholar
  17. 17.
    Goguen, J.D., Bugge, T., and Degen, J.L., Methods, 2001, vol. 21(2), pp. 179–183.CrossRefGoogle Scholar
  18. 18.
    Leo, J.C. and Skurnik, M., Adv. Exp. Med. Biol., 2011, vol. 715, pp. 1–15.CrossRefPubMedGoogle Scholar
  19. 19.
    Lu, Q., Wang, J., Faghihnejad, A., Zeng, H., and Liu, Y., Soft Matter, 2011, vol. 7, pp. 9366–9379.CrossRefGoogle Scholar
  20. 20.
    Amro, N.A., Kotra, L.P., Wadu-Mesthrige, K., Bulychev, A., Mobashery, S., and Liu, G., Langmuir, 2000, vol. 16(6), pp. 2789–2796.CrossRefGoogle Scholar
  21. 21.
    Kukkonen, M., Suomalainen, M., Kyllönen, P., Lähteenmäki, K., Lång, H., Virkola, R., Helander, I.M., and Korhonen, T.K., Mol. Microbiol., 2004, vol. 51(1), pp. 215–225.CrossRefPubMedGoogle Scholar
  22. 22.
    Skurnik, M. and Bengoechea, J.A., Carbohydr. Res., 2003, vol. 338(23), pp. 2521–2529.CrossRefPubMedGoogle Scholar
  23. 23.
    Tsang, T.M., Wiese, J.S., Felek, S., Kronshage, M., and Krukonis, E.S., PLOS One, 2013, vol. 8(12), e83621.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Strauss, J., Burnham, N.A., and Camesano, T.A., J. Mol. Recogn., 2009, vol. 22(5), pp. 347–355.CrossRefGoogle Scholar
  25. 25.
    Ivanov, I.E., Kintz, E.N., Porter, L.A., Goldberg, J.B., Burnham, N.A., and Camesano, T.A., J. Bacteriol., 2011, vol. 193(5), pp. 1259–1266.CrossRefPubMedGoogle Scholar
  26. 26.
    Jones, J.F., Feick, J.D., Imoudu, D., Chukwumah, N., Vigeant, M., and Velegol, D., Appl. Environ. Microbiol., 2003, vol. 69(11), pp. 6515–6519.CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Burks, G.A., Velegol, S.B., Paramonova, E., Lindenmuth, B.E., Feick, J.D., and Logan, B.E., Langmuir, 2003, vol. 19(6), pp. 2366–2371.CrossRefGoogle Scholar
  28. 28.
    Targosz, M., Czuba, P., Biedron, R., Strus, M., Gamian, A., Marcinkiewicz, J., and Szymonski, M., Acta Phys. Pol. A, 2006, vol. 109(3), pp. 421–426.CrossRefGoogle Scholar
  29. 29.
    Targosz, M., Labuda, A., Czuba, P., Biedron, R., Strus, M., Gamian, A., Marcinkiewicz, J., and Szymonski, M., Nanomedicine, 2006, vol. 2(2), pp. 82–88.PubMedGoogle Scholar
  30. 30.
    Wei, M.T., Hua, K.F., Hsu, J., Karmenyan, A., Tseng, K.Y., Wong, C.H., Hsu, H.Y., and Chiou, A., Opt. Express, 2007, vol. 15(17), pp. 11020–11032.CrossRefPubMedGoogle Scholar
  31. 31.
    Westphal, O. and Jann, K., Methods Carbohydr. Chem., 1965, vol. 5, pp. 83–91.Google Scholar
  32. 32.
    Byvalov, A.A., Dudina, L.G., Litvinets, S.G., Novikova, O.D., Khomenko, O.D., Portnyagina, O.Yu., and Ovodov, Yu.S., Appl. Biochem. Microbiol., 2014, vol. 50(2), pp. 179–186.CrossRefGoogle Scholar
  33. 33.
    Byvalov, A.A., Dudina, L.G., Chernyad’ev, A.V., Konyshev, I.V., Litvinets, S.G., and Ovodov, Yu.S., Mol. Gen. Mikrobiol. Virusol., 2015, vol. 30(2), pp. 93–100.CrossRefGoogle Scholar
  34. 34.
    Wozniak, A., Mameren, J.V., and Ragona, S., Curr. Pharm. Biotechnol., 2009, vol. 10(5), pp. 467–473.CrossRefPubMedGoogle Scholar
  35. 35.
    Silverman, B.W., Density Estimation for Statistics and Data Analysis, London Chapman and Hall, 1986.CrossRefGoogle Scholar
  36. 36.
    Huber, M., Kalis, C., Keck, S., Jiang, Z., Georgel, P., Du, X., Shamel, L., Sovath, S., Mudd, S., and Beutler, B., Eur. J. Immunol., 2006, vol. 36(3), pp. 701–711.CrossRefPubMedGoogle Scholar
  37. 37.
    Sidorin, E.V., Kim, N.Yu., Leichenko, E.V., Anastyuk, S.D., Dmitrenok, P.S., Naberezhnykh, G.A., and Solov’eva, T.F., Biochemistry (Moscow), 2006, vol. 71(11), pp. 1278–1283.CrossRefGoogle Scholar
  38. 38.
    Makoveichuk, E., Cherepanov, P., Lundberg, S., Forsberg, A., and Olivecrona, G.J., Lipid Res., 2003, vol. 44(2), pp. 320–330.CrossRefGoogle Scholar
  39. 39.
    Skurnik, M., Adv. Exp. Med. Biol., 2003, vol. 529, pp. 187–197.CrossRefPubMedGoogle Scholar
  40. 40.
    Knirel, Y.A. and Anisimov, A.P., Acta Naturae, 2012, vol. 4(3), pp. 46–58.PubMedPubMedCentralGoogle Scholar
  41. 41.
    Holst, O., Adv. Exp. Med. Biol., 2003, vol. 529, pp. 219–228.CrossRefPubMedGoogle Scholar
  42. 42.
    Somov, G.P., Pokrovskii, V.I., Besednova, N.N., and Antonenko, F.F., Psevdotuberkulez (Pseudotuberculosis), Moscow Meditsina, 2001.Google Scholar
  43. 43.
    Bengoechea, J.A., Adv. Exp. Med. Biol., 2003, vol. 529, pp. 267–274.CrossRefPubMedGoogle Scholar
  44. 44.
    Bengoechea, J.A., Najdenski, H., and Skurnik, M., Mol. Microbiol., 2004, vol. 52(2), pp. 451–469.CrossRefPubMedGoogle Scholar
  45. 45.
    Tang, K., Guo, H., Yi, W., Tsai, M., and Wang, P.G., Biochemistry, 2007, vol. 46(42), pp. 11744–11752.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Inc. 2017

Authors and Affiliations

  • A. A. Byvalov
    • 1
    • 2
  • V. L. Kononenko
    • 3
    • 4
  • I. V. Konyshev
    • 1
    • 2
  1. 1.Vyatka State UniversityKirov, ]610000Russia
  2. 2.Institute of Physiology, Komi Research Center, Ural BranchRussian Academy of SciencesSyktyvkarRussia
  3. 3.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia
  4. 4.Dmitry Rogachev Federal Research Center of Pediatric Hematology, Oncology, and ImmunologyMoscowRussia

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