Abstract
The bacterial strain IB-1, which exhibits antagonism towards phytopathogens and stimulates the growth of agricultural plants, was isolated from the soil. Analysis of the cultural, morphological, physiological, and biochemical features and nucleotide sequences of the genes 16S rRNA and gyrB, as well as the fatty acid composition, made it possible to attribute the strain IB-1 to the genus Paenibacillus; however, the results did not provide an unambiguous conclusion on its species. The strain Paenibacillus sp. IB-1 possesses nitrogenase activity, the ability to synthesize indoleacetic acid and cytokinin-like compounds, and antagonistic activity towards phytopathogenic fungi, which indicates prospects for its use as a biological product for agricultural purposes. A high-viscous exopolysaccharide was isolated from the cultural fluid of Paenibacillus sp. IB-1. Based on the data from IR and NMR spectroscopy, it was shown to be a heteropolymer comprised of one to four linked α-L-guluronic acid and β-D-mannuronic acid residues. The exopolysaccharide was successfully tested as an adhesive for presowing treatment of barley and wheat seeds with biofungicides.
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Bianciotto, V., Andreotti, S., Balestrini, R., Bonfante, P., and Perotto, S., Eur. J. Histochem., 2001, vol. 45, pp. 39–49.
Lugtenberg, B.J.J., Dekkers, L., and Bloemberg, G.V., Annu. Rev. Phytopathol., 2001, vol. 39, pp. 461–490.
Mongiardini, E.J., Ausmees, N., Perez-Giménez, J., Althabegoiti, M.J., Quelas, J.I., Lopez-Garcia, S.L., and Lodeiro, A.R., FEMS Microbiol. Ecol., 2008, vol. 65, pp. 279–288.
Sutherland, I.W., Microbiology, 2001, vol. 147, pp. 3–9.
Vu, B., Chen, M., Crawford, R.J., and Ivanova, E.P., Molecules, 2009, vol. 14, pp. 2535–2554.
Dzerzhinskaya, I.S., Pitatel’ye sredy dlya vydeleniya i kul’tivirovaniya mikroorganizmov (Nutrient Media for Isolation and Cultivation of Microorganisms), Astrakhan’ Izd. AGTU, 2008.
Praktikum po mikrobiologii (A Practical Course of Microbiology), Netrusov, A.I, Ed., Moscow: Obrazovat.- izdat. tsentr “Akademiya,” 2005.
Wilson, K., in Current Protocols in Molecular Biology, Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K., Eds., New York: Green Publishing Associates, 1995, pp. 241–245.
Lane, D.J., in Nucleic Acid Techniques in Bacterial Systematic, Stackebrandt, E. and Goodfellow, M., Eds., Chichester: John Wiley and Sons, 1991, pp. 115–177.
Yamamoto, S. and Harayama, S., Appl. Environ. Microbiol., 1995, vol. 61, no. 3, pp. 1104–1109.
Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulus, J., Bealer, K., and Madden, T.L., BMC Bioinformatics, 2009, vol. 10, p. 421.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S., Mol. Biol. Evol., 2011, vol. 28, no. 10, pp. 2731–2739.
Saitou, N. and Nei, M., Mol. Biol. Evol., 1987, vol. 4, no. 4, pp. 406–425.
Kimura, M., J. Mol. Evol., 1980, vol. 16, no. 2, pp. 111–120.
Tamura, K. and Nei, M., Mol. Biol. Evol., 1993, vol. 10, no. 3, pp. 512–526.
Korshunova, T.Y., Mukhamatdyarova, S.R., and Loginov, O.N., Biol. Bull., 2015, vol. 42, no. 3, pp. 220–225.
Collins, M.D., Methods Microbiol., 1985, vol. 18, pp. 329–363.
Korshunova, T.Yu., Chetverikov, S.P., Mukhamatd’yarova, S.R., and Loginov, O.N., Izv. Samarsk. Nauch. Tsentra RAN, 2013, vol. 15, no. 3(5), pp. 1637–1640.
Umarov, M.M., Assotsiativnaya azotfiksatsiya (Associative Nitrogen Fixation), Moscow Izd. Mosk. Univ., 1986.
Kudoyarova, G.R., Veselov, S.Yu., Karovaiko, N.I., Gyuli-Zade, V.Z., Cheredova, E.P., Mustafina, A.R., Moshkov, I.E., and Kulaeva, A.N., Fiziol. Rast., 1990, vol. 37, no. 1, pp. 193–199.
Chetverikov, S.P. and Loginov, O.N., Microbiology (Moscow), 2009, vol. 78, no. 4, pp. 428–432.
Loginov, Ya.O., Khudaigulov, G.G., Chetverikov, S.P., Melent’ev, A.I., and Loginov, O.N., Appl. Biochem. Microbiol., 2011, vol. 47, no. 3, pp. 311–314.
Rafikova, G.F., Korshunova, T.Yu., Minnebaev, L.F., Chetverikov, S.P., and Loginov, O.N., Microbiology (Moscow), 2016, vol. 85, no. 3, pp. 333–341.
Klappenbach, J.A., Dunbar, J.M., and Schmidt, T.M., Appl. Environ. Microbiol., 2000, vol. 66, no. 4, pp. 1328–1333.
Stackebrandt, E. and Ebers, J., Microbiol. Today, 2006, vol. 33, pp. 152–155.
Shida, O., Takagi, H., Kadowaki, K., Nakamura, L.K., and Komagata, K., Int. J. Syst. Bacteriol., 1997, vol. 47, no. 2, pp. 289–298.
Yoon, J.-H., Oh, H.-M., Yoon, B.-D., Kang, K.H., and Park, Y.-H., Int. J. Syst. Evol. Microbiol., 2003, vol. 53, no. 1, pp. 295–301.
Suominen, I., Sproer, C., Kampfer, P., Rainey, F.A., Lounatmaa, K., and Salkinoja-Salonen, M., Int. J. Syst. Evol. Microbiol., 2003, vol. 53, no. 5, pp. 1369–1374.
RF Patent no. 2380906, 2010.
RF Patent no. 2514217, 2014.
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Original Russian Text © M.D. Bakaeva, S.P. Chetverikov, T.Yu. Korshunova, O.N. Loginov, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 2, pp. 204–212.
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Bakaeva, M.D., Chetverikov, S.P., Korshunova, T.Y. et al. The new bacterial strain Paenibacillus sp. IB-1: A producer of exopolysaccharide and biologically active substances with phytohormonal and antifungal activities. Appl Biochem Microbiol 53, 201–208 (2017). https://doi.org/10.1134/S0003683817020041
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DOI: https://doi.org/10.1134/S0003683817020041