Abstract
The maximal rates and effective constants of 2,6-dichlorphenolindophenol and oxygen reduction by bacterim Gluconobacter oxydans in bacterial fuel cells under different conditions were evaluated. In an open-circuit mode, the rate of 2,6-dichlorphenolindophenol reduction coupled with ethanol oxidation under oxygen and nirogen atmospheres were 1.0 and 1.1 μM s–1 g–1, respectively. In closed-circuit mode, these values were 0.4 and 0.44 μM s–1 g–1, respectively. The initial rate of mediator reduction with the use of membrane fractions of bacteria in oxygen and nitrogen atmospheres in open-circuit mode were 6.3 and 6.9 μM s–1 g–1, whereas these values in closed-circuit mode comprised 2.2 and 2.4 μM s–1 g–1, respectively. The oxygen reduction rates in the presence and absence of 2,6-dichlorphenolindophenol were 0.31 and 0.32 μM s–1 g–1, respectively. The data obtained in this work demonstrated independent electron transfer from bacterial redox centers to the mediator and the absence of competition between the redox mediator and oxygen. The results can make it possible to reduce costs of microbial fuel cells based on activity of acetic acid bacteria G. oxydans.
Similar content being viewed by others
References
Rahimnejad, M., Adhami, A., Darvari, S., Zirepour, A., and Sang-Eun, Oh., Alexandria Engineering J., 2015, vol. 54, no. 3, pp. 745–756.
Wang, L., Wang, C., Cao, X., Xiaona, Li., and Honghai, Yu., ECS Transactions, 2008, vol. 13, no. 21, pp. 43–50.
Alferov, S.V., Voevodskaya, O.A., Nguen, V.T., Ponamoreva, O.N., and Reshetilov, A.N., Sens. Sist., 2011, vol. 25, no. 4, pp. 346–351.
Lapinsonniere, L., Picot, M., and Barriere, F., Chem. Sus. Chem., 2012, no. 5, pp. 995–1005.
Osman, M.H., Shah, A.A., and Walsh, F.C., Biosens. Bioelectron., 2011, vol. 26, no. 7, pp. 3087–3102.
Part, I.I., Microbial, M.H., Osman, ShahA.A., and Walsh, F.C., Biosens. Bioelectron., 2010, vol. 26, no. 3, pp. 953–963.
Tkac, J., Svitel, J., Vostiar, I., Navratil, M., and Gemeiner, P., Bioelectrochemistry, 2009, vol. 76, nos. 1–2, pp. 53–62.
Kumar, A., Katuri, K., Lens, P., and Leech, D., Biochem. Soc. Trans., 2012, vol. 40, no. 6, pp. 1308–1314.
Indzhgiya, E., Ponamoreva, O.N., Alferov, V.A., Reshetilov, N.A., and Lo, G., Electroanalysis, 2012, vol. 24, no. 4, pp. 924–930.
Reshetilov, A.N., Kitova, A.E., Kolesov, V.V., and Yaropolov, A.I., Electroanalysis, 2015, vol. 27, no. 6, pp. 1443–1448.
Rosenbaum, M.A., Kotloski, N.J., Gralnick, J.A., and Angenent, L.T., Biotechnol. Bioeng., 2014, vol. 111, no. 4, pp. 692–699.
Timonov, A.M., Soros. Obrazovat. Zh., 2000, vol. 6, no. 8, pp. 69–75.
Reshetilov, A.N., Alferov, S.V., Tomashevskaya, L.G., and Ponamoreva, O.N., Electroanalysis, 2006, vol. 18, nos. 19–20, pp. 2030–2034.
Roller, S.D., Bennetto, H.P., and Delaney, G.M., et al., J. Chem. Technol. Biotechnol., 1984, vol. 34 B, pp. 13–27.
Alferov, S.V., Tomashevskaya, L.G., Ponamoreva, O.N., Bogdanovskaya, V.A., and Reshetilov, A.N., Elektrokhimiya, 2006, vol. 42, no. 4, pp. 456–457.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © S.V. Alferov, S.V. Vozchikova, V.A. Arlyapov, V.A. Alferov, A.N. Reshetilov, 2017, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2017, Vol. 53, No. 2, pp. 244–250.
Rights and permissions
About this article
Cite this article
Alferov, S.V., Vozchikova, S.V., Arlyapov, V.A. et al. Competition between redox mediator and oxygen in the microbial fuel cell. Appl Biochem Microbiol 53, 267–272 (2017). https://doi.org/10.1134/S0003683817020028
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683817020028