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Respiration activity of suspension cell culture of Polyscias filicifolia bailey, Stephania glabra (Roxb.) miers, and Dioscorea deltoidea wall

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Abstract

Peculiarities of respiration of cells cultures producing biologically active compounds (isoprenoids and alkaloids) were investigated in order to optimize productivity of culture growth and biosynthesis. It had been revealed that studied cells cultures of Dioscorea deltoidea Wall (producer of furistanol glycosides), Stephania glabra (Roxb.) Miers (producer of stepharin alkaloid) and Polyscias filicifolia Bailey (complex of biologically active agents) differ both in joint respiration activity and in ratio between cytochrome and cyanide-resistant respiration, while changes of rate of total oxygen consumption and activity of alternative oxidase during growth were found to be individual for every investigated culture. Maximum rate of oxygen consumption for cells of D. deltoidea and S. glabra was marked in the period preceding active synthesis of secondary metabolites (lag phase for D. deltoidea and exponential phase for S. glabra). The revealed trends can be used for further monitoring and regulation of growth and biosynthesis of secondary metabolites in producing cell cultures during deep cultivation.

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References

  1. DiCosmo, F. and Misawa, M., Biotechnol. Adv., 1995, no. 3, pp. 425–453.

  2. Kieran, P.M., MacLoughlin, P.F., and Malone, D.M., J. Biotechnol., 1997, vol. 59, no. 1, pp. 39–52.

    Article  CAS  PubMed  Google Scholar 

  3. Verpoorte, R., Contin, A., and Memelink, J., Phytochem. Rev., 2002, no. 1, pp. 13–25.

  4. Day, D.A., Arron, G.P., and Laties, G.G., in Biochemistry of Plants, Davies, D.D.N., Ed., New-York: Academic Press, 1980, vol. 2, pp. 197–241.

    Google Scholar 

  5. Geraldes-Laakso, S. and Arrabaca, J.D., Plant Physiol. Biochem., 1997, vol. 35, no. 11, pp. 897–903.

    CAS  Google Scholar 

  6. Wagner, A.M. and Moore, A.L., Biosci. Rep., 1997, vol. 17, no. 3, pp. 319–333.

    Article  CAS  PubMed  Google Scholar 

  7. Parsons, H.L., Yip, J.Y.H., and Vanlerberghe, G.C., Plant Physiol., 1999, vol. 121, no. 4, pp. 1309–1320.

    Article  CAS  PubMed  Google Scholar 

  8. Shugaev, A.G. and Sokolova, S.V., Fiziol. Rast., 2001, vol. 48, no. 1, pp. 55–61.

    Google Scholar 

  9. Golovko, T.K. and Pystina, N.V., Fiziol. Rast., 2001, vol. 48, no. 6, pp. 846–853.

    Google Scholar 

  10. Shugaev, A.G., Shugaeva, N.A., and Vyskrebentseva, E.I., Fiziol. Rast., 2006, vol. 53, no. 4, pp. 503–510.

    Google Scholar 

  11. Duque, P. and Arrabaca, J.D., Physiol. Plant., 1999, vol. 107, no. 1, pp. 24–31.

    Article  CAS  Google Scholar 

  12. Rakhmankulova, Z.F., Fedyaev, V.V., Podashevka, O.A., and Usmanov, I.Yu., Fiziol. Rast., 2003, vol. 50, no. 2, pp. 206–212.

    CAS  Google Scholar 

  13. Clifton, R., Lister, R., Parker, K.L., Sappl, P.G., Elhafez, D., Millar, A.H., Day, D.A., and Whelan, J., Plant. Mol. Biol., 2005, vol. 58, no. 2, pp. 193–212.

    Article  CAS  PubMed  Google Scholar 

  14. Ferreira, A.L., Arrabaca, J.D., Vaz-Pinto, V., and Lima-Costa, M.E., Biol. Plant., 2008, vol. 52, no. 1, pp. 66–71.

    Article  CAS  Google Scholar 

  15. Nosov, A.M., Fiziol. Rast., 1999, vol. 46, no. 6, pp. 837–844.

    Google Scholar 

  16. McDonald, A.E., Sieger, St.M., and Vanlerberghe, G.C., Physiol. Plant., 2002, vol. 116, no. 2, pp. 135–143.

    Article  CAS  PubMed  Google Scholar 

  17. Robson, C.A. and Vanlerberghe, G.C., Plant Physiol., 2002, vol. 129, no. 4, pp. 1908–1920.

    Article  CAS  PubMed  Google Scholar 

  18. Seletzky, J.M., Noack, U., Hahn, S., Knoll, A., Amoabediny, G., and Buchs, J., J. Ind. Microbiol. Biotechnol., 2007, vol. 34, no. 2, pp. 123–130.

    Article  CAS  PubMed  Google Scholar 

  19. Vasil'eva, I.S., Vorob'ev, A.S., Gorskaya, N.V., Lipskii, A.Kh., Gurielidze, K.G., and Paseshnichenko, V.A., Prikl. Biokhim. Mikrobiol., 1987, vol. 28, no. 5, pp. 692–697.

    Google Scholar 

  20. Davydenkov, V.N., Tareeva, N.V., Kir'yanov, A.A., and Bondarenko, L.T., Khim.-Farm. Zh., 1988, vol. 2, no. 3, pp. 326–328.

    Google Scholar 

  21. Lipskii, A.Kh. and Chernyak, N.D., Fiziol. Rast., 1983, vol. 30, no. 3, pp. 437–447.

    Google Scholar 

  22. Oreshnikov, A.V., Nosov, A.M., and Manakov, M.N., Fiziol. Rast., 1994, vol. 41, no. 6, pp. 918–922.

    Google Scholar 

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    O. V. Reshetnyak, I. E. Kulichenko & A. V. Oreshnikov

  2. Biological Faculty, Moscow State University, Moscow, 119992, Russia

    M. V. Titova, E. A. Berkovich & A. M. Nosov

Authors
  1. M. V. Titova
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  2. E. A. Berkovich
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  3. O. V. Reshetnyak
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  4. I. E. Kulichenko
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  5. A. V. Oreshnikov
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  6. A. M. Nosov
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Correspondence to M. V. Titova.

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Original Russian Text © M.V. Titova, E.A. Berkovich, O.V. Reshetnyak, I.E. Kulichenko, A.V. Oreshnikov, A.M. Nosov, 2011, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 1, pp. 95–101.

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Titova, M.V., Berkovich, E.A., Reshetnyak, O.V. et al. Respiration activity of suspension cell culture of Polyscias filicifolia bailey, Stephania glabra (Roxb.) miers, and Dioscorea deltoidea wall. Appl Biochem Microbiol 47, 87–92 (2011). https://doi.org/10.1134/S0003683811010170

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  • DOI: https://doi.org/10.1134/S0003683811010170

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