Heterotrophic Bacterioplankton of the Laptev and East Siberian Sea Shelf Affected by Freshwater Inflow Areas


The present study examined the hydrochemical and biological parameters influencing the spatial distribution of bacterioplankton in the Laptev Sea and the East Siberian Sea shelf regions affected by the Khatanga, Indigirka and Kolyma rivers runoff during September 2017. The highest bacterial abundance and the amount of actively respiring bacteria were determined in rivers delta and in surface water along the edge of continental shelf. Despite the expectations based on previous studies salinity was not the determining factor affecting spatial distribution and activity of bacterioplankton. Bacterial abundance and biomass were significantly and positively correlated with chlorophyll “a” and pheophytin content.

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  1. 1

    O. A. Alekin and Yu. I. Lyakhin, Chemistry of an Ocean (Gidrometeoizdat, Leningrad, 1984) [in Russian].

    Google Scholar 

  2. 2

    A. B. Demidov and V. I. Gagarin, “Primary production and associated environmental conditions in the East Siberian Sea in autumn,” Dokl. Earth Sci. 487, 1006–1011 (2019).

    Article  Google Scholar 

  3. 3

    A. B. Demidov, V. I. Gagarin, E. G. Arashkevich, et al., “Spatial variability of primary production and chlorophyll in the Laptev Sea in August–September,” Oceanology (Engl. Transl.) 59, 755–770 (2019).

  4. 4

    A. I. Kopylov, A. F. Sazhin, E. A. Zabotkina, et al., “Viruses, bacteria, and heterotrophic nanoflagellates in Laptev Sea plankton,” Oceanology (Engl. Transl.) 56, 789–798 (2016).

  5. 5

    K. N. Kosobokova, Zooplankton of the Arctic Basin: Structure of Communities, Ecology, and Distribution Pattern (GEOS, Moscow, 2012) [in Russian].

    Google Scholar 

  6. 6

    S. A. Lapin, Candidate’s Dissertation in Geography (Moscow State Univ., Moscow, 2012).

  7. 7

    P. N. Makkaveev, A. A. Polukhin, A. M. Seliverstova, et al., “Dynamics of biogenic elements in near-estuarine area of the Lena River: expedition results of 2015 and 2017,” Arkt.: Ekol. Ekon., No. 2 (30), 56–67 (2018).

  8. 8

    V. N. Mikhailov, Estuaries of Russian Rivers and Adjacent Countries: Past, Present, and Future (GEOS, Moscow, 1997) [in Russian].

    Google Scholar 

  9. 9

    I. V. Mosharova, V. V. Il’inskii, and S. A. Mosharov, “State of heterotrophic bacterioplankton of Yenisei estuary and the zone of Ob–Yenisei discharge in autumn in relation with environmental factors,” Water Resour. 43, 341–352 (2016).

    Article  Google Scholar 

  10. 10

    N. D. Romanova and A. F. Sazhin, “Bacterioplankton of the Kara Sea shelf,” Oceanology (Engl. Transl.) 55, 858–862 (2015).

  11. 11

    N. D. Romanova and A. F. Sazhin, “Relationships between the cell volume and the carbon content of bacteria,” Oceanology (Engl. Transl.) 50, 522–530 (2010).

  12. 12

    N. D. Romanova and A. F. Sazhin, “Methodological aspects of the determination of the bacterioplankton number, biomass, and production,” Oceanology (Engl. Transl.) 51, 518–527 (2011).

  13. 13

    RD 52.10.242-92. Guide for Chemical Analysis of Marine Waters (Gidrometeoizdat, St. Petersburg, 1993) [in Russian].

  14. 14

    Manual for Chemical Analysis of Marine and Fresh Waters in Ecological Monitoring of Fishery Reservoirs and Prospective for Fishery Areas of the World Ocean, Ed. by V. V. Sapozhnikov (VNIRO, Moscow, 2003) [in Russian].

    Google Scholar 

  15. 15

    A. S. Savvichev, E. E. Zakharova, E. F. Veslopolova, et al., “Microbial processes of the carbon and sulfur cycles in the Kara Sea,” Oceanology (Engl. Transl.) 50, 893–908 (2010).

  16. 16

    A. F. Sazhin, N. D. Romanova, and S. A. Mosharov, “Bacterial and primary production in the pelagic zone of the Kara Sea,” Oceanology (Engl. Transl.) 50, 759–765 (2010).

  17. 17

    Modern Hydrochemical Studies of the Ocean, Ed. by O. K. Bordovskii and V. N. Ivanenkov (Shirshov Institute of Oceanography, Russian Academy of Sciences, Moscow, 1992) [in Russian].

    Google Scholar 

  18. 18

    M. V. Flint, E. G. Arashkevich, V. A. Atem’ev, et al., Ecosystems of the Seas of Siberian Arctic: Materials of Expeditions of 2015 and 2017 (Shirshov Institute of Oceanography, Russian Academy of Sciences, Moscow, 2018) [in Russian].

    Google Scholar 

  19. 19

    M. V. Flint, S. G. Poyarkov, and N. A. Rymsky-Korsakov, “Ecosystems of the Siberian Arctic seas-2017 (cruise 69 of the R/V Akademik Mstislav Keldysh),” Oceanology (Engl. Transl.) 58, 315–318 (2018).

  20. 20

    K. Aagaard and E.C. Carmack, “The role of sea ice and other fresh water in the Arctic circulation,” J. Geophys. Res.: Oceans 94, 14485–14498 (1989).

    Article  Google Scholar 

  21. 21

    B. Bluhm, K. N. Kosobokova, and E. Carmack, “A tale of two basins: an integrated physical and biological perspective of the deep Arctic Ocean,” Prog. Oceanogr. 139, 89–121 (2015).

    Article  Google Scholar 

  22. 22

    J. J. Cole, S. Findley, and M. L. Pace, “Bacterial production in fresh and saltwater ecosystems,” Mar. Ecol.: Prog. Ser. 43, 1–10 (1988).

    Article  Google Scholar 

  23. 23

    T. Fenchel, “The microbial loop—25 years later,” J. Exp. Mar. Biol. Ecol. 366 (1), 99–103 (2008).

    Article  Google Scholar 

  24. 24

    M. E. Garneau, W. F. Vincent, R. Terrado, et al., “Importance of particle-associated bacterial heterotrophy in a coastal Arctic ecosystem,” J. Mar. Syst. 75, 185–197 (2009).

    Article  Google Scholar 

  25. 25

    E. Kamia, S. Izumiyama, M. Nishimura, et al., “Effects of fixation and storage on flow cytometric analysis of marine bacteria,” J. Oceanol. 63, 101–112 (2007).

    Article  Google Scholar 

  26. 26

    D. L. Kirchman, R. R. Malmstrom, and M. T. Cottrell, “Control of bacterial growth by temperature and organic matter in the Western Arctic,” Deep Sea Res., Part II 52 (24), 3386–3395 (2005).

    Article  Google Scholar 

  27. 27

    J. W. McClelland, R. M. Holmes, K. H. Dunton, et al., “The Arctic Ocean estuary,” Estuaries Coasts 35, 353–368 (2011).

    Article  Google Scholar 

  28. 28

    B. Meon and R. M. W. Amon, “Heterotrophic bacterial activity and fluxes of dissolved free amino acids and glucose in the Arctic rivers Ob, Yenisei and the adjacent Kara Sea,” Aquat. Microb. Ecol. 37, 121–135 (2004).

    Article  Google Scholar 

  29. 29

    M. M. Sala, J. M. Arrieta, J. A. Boras, et al., “The impact of ice melting on bacterioplankton in the Arctic Ocean,” Polar Biol. 33 (12), 1683–1694 (2010).

    Article  Google Scholar 

  30. 30

    A. Saliot, G. Cauwet, G. Cahet, et al., “Microbial activities in the Lena River delta and Laptev Sea,” Mar. Chem. 53, 247–254 (1996).

    Article  Google Scholar 

  31. 31

    B. Sherr, E. Sherr, and P. del Giorgio, “Enumeration of total and highly active bacteria,” Methods Microbiol. 30, 129–160 (2001).

    Article  Google Scholar 

  32. 32

    Yu. I. Sorokin and P. Yu. Sorokin, “Plankton and primary production in the Lena River estuary and in the south-eastern Laptev Sea,” Estuarine, Coastal Shelf Sci. 43, 399–418 (1996).

    Article  Google Scholar 

  33. 33

    A. M. Springer, C. P. Mcroy, and M. V. Flint, “The Bering Sea Green Belt: shelf-edge processes and ecosystem production,” Fish. Oceanogr. 5, 205–223 (1996).

    Article  Google Scholar 

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This study was supported by the Russian Science Foundation (project no. 17-77-10138). The research was carried out as part of state task no. 0128-2019-0011.

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Correspondence to E. M. Bezzubova.

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Translated by A. Panyushkina

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Bezzubova, E.M., Seliverstova, A.M., Zamyatin, I.A. et al. Heterotrophic Bacterioplankton of the Laptev and East Siberian Sea Shelf Affected by Freshwater Inflow Areas. Oceanology 60, 62–73 (2020). https://doi.org/10.1134/S0001437020010026

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  • bacterioplankton
  • estuaries
  • Laptev Sea
  • East Siberian Sea
  • Arctic ecosystems
  • bacteria