Changes in the Activity of Glutathione Peroxidase in the Blood Plasma and Serum of Rats during Postnatal Development and Aging
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The activity of glutathione peroxidase (GPx) in plasma and serum of Wistar rats during postnatal ontogeny and aging is studied. A combination of various substrates is used for assessment of the enzymatic activity. During the period of milk nutrition, since the beginning of the consumption of solid food, GPx activity is rapidly increasing and almost reaches the adult level by 1.5 months. Enzyme activity remains stable in adulthood and has long been high in aging rats. A decrease in GPx activity around 23–26 months of age is revealed, with reduced homocysteine (but not with reduced glutathione) as a GPx substrate. The dramatic increase of GPx activity in plasma from 0.5 to 1–1.5 months of age may be an adaptation to consumption of solid food that may contain substances inducing oxidative stress. Probably, with sufficient selenium consumption, a decrease in GPx activity in rat plasma occurs only in extreme old age and can be considered as an indicator of physiological aging.
Keywords:glutathione peroxidase postnatal ontogeny aging homocysteine hydrogen peroxide tert-butyl hydroperoxide Ellman’s reagent
The authors thank prof. A.V. Arutunyan (Ott Research Institute of Obstetrics, Gynecology and Reproductology) for useful discussions on the GPx3 substrate specificity and M.O. Matrosova (Peter the Great St. Petersburg Polytechnic University) and N.A. Pechnikova (St. Petersburg State Chemical Pharmaceutical University) for the help with animal care.
COMPLIANCE WITH ETHICAL STANDARDS
Conflict of interests. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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