Age-Related Changes in the Functional Indices of Cardiac Mitochondria in Chronic Alcohol Intoxication in Rats
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The effect of chronic continuous consumption of 5 and 10% ethanol solutions for six months on the respiratory function and oxidant/antioxidant status in the cardiac mitochondria of rats of different gender and age is studied. A decrease in the rate of oxygen consumption by cardiomyocyte mitochondria under the V2, V3, and V4 metabolic conditions according to Chance during the activation of the respiratory chain complexes I, I + II, and II is observed in old animals (24-month-old) compared to young (11-month-old) rats. The level of the products of lipid peroxidation (LPO) (malondialdehyde) in the cardiomyocyte mitochondria increases, whereas the activity of the antioxidant enzymes (superoxide dismutase and glutathione peroxidase) decreases. Chronic alcoholization of 24-month-old rats of both genders results in a more pronounced decline in the activity of respiratory function of the cardiac mitochondria, disintegration of respiration and oxidative phosphorylation, reduced activity of antiradical protection enzymes, and an increase in the level of LPO products compared to younger rats.
Keywords:mitochondrial respiration lipid peroxidation intensity age chronic alcohol intoxication
COMPLIANCE WITH ETHICAL STANDARDS
The authors declare that they have no conflict of interest.
The animal care procedure corresponded to the Order of the Ministry of Health of the Russian Federation, April 1, 2016, no. 199n “On the Approval of the Rules of Good Laboratory Practice” and the international recommendations of the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (The European Convention, 1986).
The study was performed in accordance with the rules indicated in the order and the requirements of the Directive 2010/63/EU of the European Parliament and of the Council of September 22, 2010 on the protection of animals used for scientific purposes. The protocol of the experiment was approved by the independent regional Ethics Committee (Volgograd Medical Research Center, project no. 2034–2017 from September 15, 2017).
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