Advertisement

Nanotechnologies in Russia

, Volume 13, Issue 7–8, pp 393–399 | Cite as

Morphological Changes in Lung Tissues of Mice Caused by Exposure to Nano-Sized Particles of Nickel Oxide

  • N. V. Zaitseva
  • M. A. ZemlyanovaEmail author
  • A. M. Ignatova
  • M. S. Stepankov
Nanobiology
  • 6 Downloads

Abstract

The authors detected nidal perivascular and peribronchial lymphoid infiltration with macrophages and eosinophils admixtures in lung tissues of BALB/C mice with body weight equal to 25–30 g after a single 4-h inhalation exposure to nickel oxide, the size of its particles being 17–40 nm, and the actual concentration of the compound being equal to 1.34 ± 0.07 mg/dm3. Such changes occurred only in the experimental group and nothing similar was detected either in mice from the comparative group that had been exposed to macrodisperse nickel oxide or in mice from the reference group. Changes in alveolar patterns were examined via fractal analysis of images; the examination results revealed that more apparent changes in fractal dimension occurred under exposure to nanoparticles of nickel oxide. Fractal dimension of the alveolar pattern in the lungs of mice from the experimental group was 7% higher than in the reference group, and 4% higher than in the comparative group. Fractal dimension was the highest for those parts of the lungs where lymphoid infiltration occurred; it was 11% higher than the same parameter in the reference group, and 7% higher than in the comparative group. The greatest number of alveolar elements with their sphericity coefficient being equal to 0.7–0.8 was observed in the reference group; this parameter decreased in both comparative and experimental groups, but in both groups there was an increase in a number of elements with sphericity coefficient being equal to 0.4–0.5, and there were even significantly deformed elements with the coefficient being equal to 0.2. The greatest dispersity factor value was detected in the reference group; the lowest dispersity factor value, in the experimental group. The changes the authors revealed in lung tissues prove that nano-sized nickel oxide particles are more toxic than those of micro-dispersed analogue.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D. V. Makarov, “Global market forecast nanopowders,” Vestn. KRAUNTs, Fiz.-Mat. Nauki 1 (8), 97–102 (2014).Google Scholar
  2. 2.
    “Nickel(II) oxide nanopowder, <50 nm particle size (TEM), 99.8% trace metals,” Material Safety Data Sheet (MSDS) (Sigma-Aldrich, 2014).Google Scholar
  3. 3.
    M. Horie, H. Fukui, Sh. Endoh, J. Maru, A. Miyauchi, M. Shichiri, K. Fujita, E. Niki, Y. Hagihara, Y. Yoshida, Y. Morimoto, and H. Iwahashi, “Comparison of acute oxidative stress on rat lung induced by nano and finescale, soluble and insoluble metal oxide particles: NiO and TiO2,” Inhalation Toxicol. 24, 391–400 (2012).CrossRefGoogle Scholar
  4. 4.
    J. R. Pietruska, X. Liu, A. Smith, K. McNeil, P. Weston, A. Zhitkovich, R. Hurt, and A. B. Kane, “Intracellular mobilization of nickel, and HIF-1α activation in human lung epithelial cells exposed to metallic nickel and nickel oxide nanoparticles,” Toxicol. Sci. 124, 138–148 (2011).CrossRefGoogle Scholar
  5. 5.
    A. Munoz and M. Costa, “Elucidating the mechanisms of nickel compound uptake: a review of particulate and nano-nickel endocytosis and toxicity,” Toxicol. Appl. Pharmacol. 260, 1–16 (2012).CrossRefGoogle Scholar
  6. 6.
    L. Capasso, M. Camatini, and M. Gualtieri, “Nickel oxide nanoparticles induce inflammation and genotoxic effect in lung epithelial cells,” Toxicol. Lett. 226, 28–34 (2014).CrossRefGoogle Scholar
  7. 7.
    K. Nishi, Y. Morimoto, A. Ogami, M. Murakami, T.Myojo, T. Oyabu, C. Kadoya, M. Yamamoto, M. Todoroki, M. Hirohashi, S. Yamasaki, K. Fujita, S. Endo, K. Uchida, K. Yamamoto, J. Nakanishi, and I. Tanaka, “Expression of cytokine-induced neutrophil chemoattractant in rat lungs by intratracheal instillation of nickel oxide nanoparticles,” Inhalation Toxicol. 21, 1030–1039 (2009).CrossRefGoogle Scholar
  8. 8.
    M. Horie, K. Nishio, K. Fujita, H. Kato, A. Nakamura, S. Kinugasa, S. Endoh, A. Miyauchi, K. Yamamoto, H. Murayama, E. Niki, H. Iwahashi, Y. Yoshida, and J. Nakanishi, “Ultrafine NiO particles induce cytotoxicity in vitro by cellular uptake and subsequent Ni(II) release,” Chem. Res. Toxicol. 22, 1415–1426 (2009).CrossRefGoogle Scholar
  9. 9.
    Y. Mizuguchi, T. Myojo, T. Oyabu, M. Hashiba, B. W. Lee, M. Yamamoto, M. Todoroki, K. Nishi, C. Kadoya, A. Ogami, Y. Morimoto, I. Tanaka, M. Shimada, K. Uchida, S. Endoh, and J. Nakanishi, “Comparison of dose-response relations between 4-week inhalation and intratracheal instillation of NiO nanoparticles using polimorphonuclear neutrophils in bronchoalveolar lavage fluid as a biomarker of pulmonary inflammation,” Inhalation Toxicol. 25, 29–36 (2013).CrossRefGoogle Scholar
  10. 10.
    H. M. Braakhuis, M. V. Park, I. Gosens, W. H. Jong, and F. R. Cassee, “Physicochemical characteristics of nanomaterials that affect pulmonary inflammation,” Part Fibre Toxicol. 11 (18), 1–25 (2014).Google Scholar
  11. 11.
    V. V. Isaeva, Yu. A. Karetin, A. V. Chernyshev, and D. Yu. Shkuratov, Fractals and Chaos in Biological Morphogenesis (Vladivostok, 2004) [in Russian].Google Scholar
  12. 12.
    R. L. Adam, R. C. Silva, F. G. Pereira, N. J. Leite, I. Lorand-Metze, and K. Metze, “The fractal dimension of nuclear chromatin as a prognostic factor in acute precursor B lymphoblastic leukemia,” Cell Oncol. 28, 55–59 (2006).Google Scholar
  13. 13.
    A. Mashiah, O. Wolach, J. Sandbank, O. Uziel, P. Raanani, and M. Lahav, “Lymphoma and leukemia cells possess fractal dimensions that correlate with their interpretation in terms of fractal biological features,” Acta Haematol. 119, 142–150 (2008).CrossRefGoogle Scholar
  14. 14.
    S. V. Muniandy and J. Stanlas, “Modelling of chromatin morphologies in breast cancer cells undergoing apoptosis using generalized cauchy field,” Comput. Med. Imaging Graph. 32, 631–637 (2008).CrossRefGoogle Scholar
  15. 15.
    G. A. Losa, “Fractals and their contribution to biology and medicine,” Medicographia 3 (34), 364–374 (2012).Google Scholar
  16. 16.
    Guide for the Care and Use of Laboratory Animals, National Research Council of the National Academies (Natl. Acad. Press, Washington, DC, 2011).Google Scholar
  17. 17.
    S. V. Bozhokin and D. A. Parshin, Fractals and Multifractals (Regulyar. Khaotich. Dinamika, Izhevsk, 2001) [in Russian].Google Scholar
  18. 18.
    A. M. Ignatova and V. I. Vereshchagin, “Application of digital image analysis in the research and evaluation of statistical parameters of solid particles welding fumes,” Vestn. Perm. Politekh. Univ., Mashinostr., Materialoved. 19 (1), 41–57 (2017).Google Scholar
  19. 19.
    O. A. Belenko, “Effect of particle size and shape on the properties of atmospheric aerosols,” in Proceedings of the International Congress on Environmental Monitoring, Geo-Ecology, Remote Sensing of the Earth and Photogrammetry Geo-Sibir’-2006, Part 1 (SGGA, Novosibirsk, 2006), Vol. 3, pp. 163–167.Google Scholar
  20. 20.
    B. Weyn, W. Jacob, G. van de Wouwer, V. da Silva, R.Montironi, D. Thompson, H. G. Bartels, A. van Daele, and P. H. Bartels, “Fractal dimension, form and shape factors for the quantification of nuclear signature profiles,” Fractals Biol. Med., No. 3, 42–49 (2002).Google Scholar
  21. 21.
    E. R. Weibel, “Fractal geometry: a design principle for living organisms,” Am. J. Physiol. 261, 361–369 (1991).Google Scholar
  22. 22.
    M. Elsabahy and K. Wooley, “Cytokines as biomarkers of nanoparticle immunotoxicity,” Chem. Soc. Rev. 42, 5552–5576 (2013).CrossRefGoogle Scholar
  23. 23.
    Y. Mo, X. Zhu, and X. Hu, “Cytokine and NO release from peripheral blood neutrophils after exposure to metal nanoparticles: in vitro and ex vivo studies,” Nanotoxicology 2, 79–87 (2008).CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • N. V. Zaitseva
    • 1
  • M. A. Zemlyanova
    • 1
    • 2
    • 3
    Email author
  • A. M. Ignatova
    • 1
    • 3
  • M. S. Stepankov
    • 1
    • 2
  1. 1.Federal Management Center for Medical and Preventive Health Risk Management TechnologiesPermRussia
  2. 2.Perm State National Research UniversityPermRussia
  3. 3.Perm National Research Polytechnic UniversityPermRussia

Personalised recommendations