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Study of the Molecular Composition of Exhaled Breath Condensate by High-Resolution Mass Spectrometry

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Exhaled breath condensate (EBC) contains a wide range of low- and high-molecular-weight compounds and is of great research interest in the search for markers of socially significant pathologies. Due to its noninvasive, rapid, and safe collection methods, an EBC sample can serve as a diagnostic sample for use in personalized instruments of body state monitoring. The use of modern high-sensitivity methods, such as mass spectrometry, forms the basis for reliable and most complete identification of the EBC molecular profile. During study, the comparative analysis of some of these methods was performed, and the most optimum approach to molecular profiling of exhaled breath condensate using high-resolution mass spectrometry was proposed. It has been shown that data from analysis of the EBC molecular profile can depend significantly on both the type of a device used to collect EBC and the cooling temperature of the condenser. It should be noted that a large number of compounds have identified in EBC residues sorbed on the walls of collecting devices that were additionally collected using an organic solvent.

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The present work was performed using equipment and resources from the Center for Shared Use “New Materials and Technologies” at the Institute of Biochemical Physics of the Russian Academy of Sciences.


This work was financially supported by the Russian Foundation for Basic Research (project no. 18-29-09158 MK).

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Correspondence to A. S. Kononikhin.

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Translated by K. Utegenov

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Kononikhin, A.S., Zakharova, N.V., Yusupov, A.E. et al. Study of the Molecular Composition of Exhaled Breath Condensate by High-Resolution Mass Spectrometry. Russ. J. Phys. Chem. B 13, 951–955 (2019). https://doi.org/10.1134/S1990793119060216

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  • exhaled breath condensate
  • mass spectrometry
  • proteomics
  • metabolomics