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Cell and Tissue Biology

, Volume 12, Issue 6, pp 506–509 | Cite as

The Release of Membrane Vesicles with Mature MicroRNA-221 and Activated Caspase-3 by Platelets during Storage of Platelet Concentrate

  • V. V. Kishenko
  • K. A. Kondratov
  • V. Yu. Mikhailovsky
  • S. V. Sidorkevich
  • T. V. Vavilova
  • O. V. SirotkinaEmail author
  • A. V. Fedorov
Article
  • 5 Downloads

Abstract

The preparation and storage of platelet concentrate can be accompanied by activation of platelets and triggering of apoptosis, increasing the formation of platelet membrane vesicles and worsening the therapeutic properties of such preparations. However, the mechanisms for the regulation of apoptosis in platelets and the molecular composition of platelet membrane vesicles associated with this process have so far remained poorly understood. The aim of the work was to study proapoptotic caspase-3 and antiapoptotic microRNA-221 levels in platelets and platelet membrane vesicles during the storage of platelet concentrate preparations. The object of the study was samples of the pooled platelet concentrates (n = 6), taken from the containers on the second and seventh days of storage. Full length and activated caspase-3 were found in platelets and in fractions of membrane vesicles. The proportion of activated caspase-3 in platelets increased during storage. MicroRNA-221 was found in platelets, fractions of membrane vesicles, as well as in the extravesicular fraction. During storage, levels of microRNA-221 increased 8-fold (P = 0.004) in the fraction of membrane vesicles pelleted at 100 000 g. The results of the study indicate that the membrane vesicles released by platelets during storage contain mature microRNA-221 and the activated form of caspase-3.

Keywords:

platelet concentrate membrane vesicles exosomes microRNA-221 caspase-3 

Notes

ACKNOWLEDGMENTS

The study was supported by the Russian Foundation for Basic Research, project no. 16-04-01142, and was performed with the use of the equipment of the Nanotechnology Interdisciplinary Resource Center of the St. Petersburg State University.

COMPLIANCE WITH ETHICAL STANDARDS

Сonflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. This article does not contain any studies involving animals performed by any of the authors.

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. V. Kishenko
    • 1
  • K. A. Kondratov
    • 1
  • V. Yu. Mikhailovsky
    • 2
  • S. V. Sidorkevich
    • 1
  • T. V. Vavilova
    • 1
  • O. V. Sirotkina
    • 1
    • 3
    • 4
    Email author
  • A. V. Fedorov
    • 1
  1. 1.Almazov National Medical Research Center of the Ministry of Health of the Russian FederationSt. PetersburgRussia
  2. 2.St. Petersburg State University, Interdisciplinary Resource Center for NanotechnologySt. PetersburgRussia
  3. 3.Petersburg Nuclear Physics Institute Named after Konstantinov, National Research Center Kurchatov Institute,GatchinaRussia
  4. 4.Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of the Russian FederationSt. PetersburgRussia

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